E279A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
E279T/D281N |
site-directed mutagenesis of the ChBD2 domain residues, the mutant shows altered substrate binding affinity and specificity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
I337A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
N272A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
N327A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
N339A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
S306A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
S309A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
T283A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
W274A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
W308A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |
W326A |
site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain |
Pyrococcus furiosus |