Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Trichoderma viride |
Protein Variants | Comment | Organism |
---|---|---|
Y185F/S226P | site-directed mutagenesis | Trichoderma viride |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits at 13% at 0.5 mM, 14% at 2 mM | Trichoderma viride | |
Cu2+ | inhibits at 12% at 0.5 mM, 25% at 2 mM | Trichoderma viride | |
Zn2+ | inhibits at 18% at 0.5 mM, 21% at 2 mM | Trichoderma viride |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | mutant enzyme, pH 7.0, 37°C | Trichoderma viride | |
0.54 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | wild-type enzyme, pH 7.0, 37°C | Trichoderma viride |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates at 0.5-2 mM | Trichoderma viride | |
Ca2+ | activates slightly at 2 mM | Trichoderma viride | |
Mg2+ | activates at 0.5-2 mM | Trichoderma viride | |
Mn2+ | activates at 0.5-2 mM | Trichoderma viride | |
additional information | poor effects by Na+ and K+ at 0.5-2 mM | Trichoderma viride |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Trichoderma viride |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma viride | - |
- |
- |
Trichoderma viride MECH | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme 9.7fold from Escherichia coli by ammonium sulfate precipitation, anion exchange chromatography, concentration by PEG 20000, and gel filtration | Trichoderma viride |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Trichoderma viride | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Trichoderma viride MECH | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Trichoderma viride |
Synonyms | Comment | Organism |
---|---|---|
Ech42 | - |
Trichoderma viride |
endochitinase | - |
Trichoderma viride |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type and mutant enzymes | Trichoderma viride |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, wild-type and mutant enzymes, profile overview | Trichoderma viride |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | pH 7.0, 10 min, purified mutant enzyme, stable at, over 90% activity within this range | Trichoderma viride |
20 | 70 | pH 7.0, 10 min, purified wild-type enzyme, stable at, over 90% activity within this range | Trichoderma viride |
80 | - |
pH 7.0, 10 min, purified mutant enzyme, loss of 50% activity | Trichoderma viride |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
wild-type and mutant enzymes | Trichoderma viride |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, wild-type and mutant enzymes, profile overview | Trichoderma viride |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 37°C, the purified wild-type enzyme shows over 80% activity within this range, the purified mutant shows over 90% activity | Trichoderma viride |