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Literature summary for 3.2.1.14 extracted from

  • Fu, X.; Yan, Q.; Wang, J.; Yang, S.; Jiang, Z.
    Purification and biochemical characterization of novel acidic chitinase from Paenicibacillus barengoltzii (2016), Int. J. Biol. Macromol., 91, 973-979 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 37% residual activity at 1 mM Paenibacillus barengoltzii
Ba2+ 84% residual activity at 1 mM Paenibacillus barengoltzii
Co2+ 78% residual activity at 1 mM Paenibacillus barengoltzii
Cr2+ 56% residual activity at 1 mM Paenibacillus barengoltzii
Fe3+ 83% residual activity at 1 mM Paenibacillus barengoltzii
Hg2+ 35% residual activity at 1 mM Paenibacillus barengoltzii
Mn2+ 80% residual activity at 1 mM Paenibacillus barengoltzii
additional information EDTA and SDS show no significant effects on chitinase activity; EDTA and SDS show no significant effects on enzyme activity Paenibacillus barengoltzii
Ni2+ 52% residual activity at 1 mM Paenibacillus barengoltzii
Sn2+ 80% residual activity at 1 mM Paenibacillus barengoltzii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 116% activity at 1 mM Paenibacillus barengoltzii
Mg2+ 124% activity at 1 mM Paenibacillus barengoltzii
additional information Cu2+, Zn2+ and K+ show no significant effects on chitinase activity Paenibacillus barengoltzii
additional information Cu2+, Zn2+ and K+ show no significant effects on enzyme activity Paenibacillus barengoltzii
Na+ 115% activity at 1 mM Paenibacillus barengoltzii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67900
-
 gel filtration Paenibacillus barengoltzii

Organism

Organism UniProt Comment Textmining
Paenibacillus barengoltzii A0A0F6NWE3
-
-
Paenibacillus barengoltzii CAU904 A0A0F6NWE3
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Paenibacillus barengoltzii
Q Sepharose column chromatography, and Superdex 200 gel filtration Paenibacillus barengoltzii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + H2O 2.1% activity compared to colloidal chitin Paenibacillus barengoltzii ?
-
 ?
beta-chitin + H2O 4.3% activity compared to colloidal chitin Paenibacillus barengoltzii ?
-
 ?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + 2 H2O
-
 Paenibacillus barengoltzii 2 beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Paenibacillus barengoltzii beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?
beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Paenibacillus barengoltzii beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?
carboxymethyl cellulose + H2O 3.5% activity compared to colloidal chitin Paenibacillus barengoltzii ?
-
 ?
carboxymethyl cellulose + H2O 3.5% activity compared to colloidal chitin Paenibacillus barengoltzii CAU904 ?
-
 ?
colloidal chitin + H2O 100% activity Paenibacillus barengoltzii beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
colloidal chitin + H2O 100% activity Paenibacillus barengoltzii N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + tetra-N-acetyl-beta-D-glucosamine
-
 ?
glycol chitin + H2O 89.4% activity compared to colloidal chitin Paenibacillus barengoltzii ?
-
 ?
glycol chitin + H2O 89.4% activity compared to colloidal chitin Paenibacillus barengoltzii CAU904 ?
-
 ?
glycol chitin + H2O 89.4% activity compared to colloidal chitin Paenibacillus barengoltzii N-acetyl-beta-D-glucosamine + ?
-
 ?
additional information no or trace activity with N,N'-diacetyl-beta-D-chitobiose, powdery chitin, soluble starch, and 4-nitrophenyl 2-acetamido-2-deoxy-beta-glucopyranoside Paenibacillus barengoltzii ?
-
 ?
additional information the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose Paenibacillus barengoltzii ?
-
 ?
additional information the enzyme shows no activity with chitosan, soluble starch and 4-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside Paenibacillus barengoltzii ?
-
 ?
additional information no or trace activity with N,N'-diacetyl-beta-D-chitobiose, powdery chitin, soluble starch, and 4-nitrophenyl 2-acetamido-2-deoxy-beta-glucopyranoside Paenibacillus barengoltzii CAU904 ?
-
 ?
additional information the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose Paenibacillus barengoltzii CAU904 ?
-
 ?
additional information the enzyme shows no activity with chitosan, soluble starch and 4-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside Paenibacillus barengoltzii CAU904 ?
-
 ?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
-
 Paenibacillus barengoltzii N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
 ?
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
-
 Paenibacillus barengoltzii N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
 ?
N,N',N''-triacetyl-beta-D-chitotriose + H2O
-
 Paenibacillus barengoltzii N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Paenibacillus barengoltzii

Synonyms

Synonyms Comment Organism
acidic chitinase
-
 Paenibacillus barengoltzii
Chi67
-
 Paenibacillus barengoltzii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Paenibacillus barengoltzii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 the enzyme is stable up to 55°C with a thermal denaturing half-life of 43, 102 and 658 min at 65°C, 60°C, and 55°C, respectively Paenibacillus barengoltzii
55 65 the enzyme os stable at temperatures below 55°C, since more than 85% of its original activities are retained after incubation at different temperatures for 30 min. The thermal denaturation half-lives of the enzyme at 55, 60 and 65°C are 658, 102 and 43 min, respectively Paenibacillus barengoltzii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 glycol chitin at pH 3.5 and 60°C Paenibacillus barengoltzii
0.019
-
 colloidal chitin at pH 3.5 and 60°C Paenibacillus barengoltzii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Paenibacillus barengoltzii
3.5
-
 in 50 mM phosphate-citrate buffer Paenibacillus barengoltzii

pH Range

pH Minimum pH Maximum Comment Organism
3 9 more than 80% activity between pH 3.0 and 9.0 Paenibacillus barengoltzii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 the enzyme displays broad pH stability range in pH 3.0-9.0 as more than 80% of its activities are maintained after 30 min incubation Paenibacillus barengoltzii