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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of hydrolase with chitinase and chitosanase activity from commercial stem bromelain (2002), J. Agric. Food Chem., 50, 4666-4673.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Ananas comosus |  |
| Hg2+ | - |
Ananas comosus | |
| N-bromosuccinimide | - |
Ananas comosus | |
| p-hydroxymercuribenzoate | - |
Ananas comosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15800 | - |
2 * 15800, SDS-PAGE | Ananas comosus |
| 31400 | - |
gel filtration | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Ananas comosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O | - |
Ananas comosus | ? | - |
? | |
| chitopentaose + H2O | - |
Ananas comosus | chitobiose + chitotriose + D-glucosamine | - |
? | |
| chitosan + H2O | - |
Ananas comosus | glucosamine + chitobiose + chitooligomers with a chain length longer than 6 | - |
? | |
| chitotetraose + H2O | - |
Ananas comosus | chitobiose + chitotriose + D-glucosamine | - |
? | |
| colloidal chitin + H2O | - |
Ananas comosus | ? | - |
? | |
| glycol chitin + H2O | - |
Ananas comosus | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 15800, SDS-PAGE | Ananas comosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
with chitosan or 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside as substrate | Ananas comosus |
| 60 | - |
with glycol chitin as substrate | Ananas comosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
with chitosan as substrate | Ananas comosus |
| 3.5 | 5.5 | with colloidal chitin as substrate | Ananas comosus |
| 4 | - |
with glycol chitin or 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside as substrate | Ananas comosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ananas comosus | isoelectric focusing | - |
6 |
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