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Literature summary for 3.2.1.14 extracted from

  • Andersen, M.D.; Jensen, A.; Robertus, J.D.; Leah, R.; Skriver, K.
    Heterologous expression and characterization of wild-type and mutant forms of a 26 kDa endochitinase from barley (Hordeum vulgare L.) (1997), Biochem. J., 322, 815-822.
No PubMed abstract available
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
E67Q undetectable enzyme activity Hordeum vulgare
E89Q 0.25% of wild-type activity remains Hordeum vulgare
N124A 0.82% of wild-type activity remains Hordeum vulgare
Q118A 76% of wild-type activity remains Hordeum vulgare
Y123A 15% of wild-type activity remains Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant endochitinase to homogeneity, 92fold Hordeum vulgare

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylated-chitin-remazol brilliant violet + H2O
-
 Hordeum vulgare ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Hordeum vulgare