Literature summary for 3.2.1.14 extracted from

Ohnuma, T.; Numata, T.; Osawa, T.; Mizuhara, M.; Lampela, O.; Juffer, A.H.; Skriver, K.; Fukamizo, T.
A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis (2011), Planta, 234, 123-137.

Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
gene chiC, quantitative real-time PCR expression analysis, recombinant expression in Escherichia coli	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant AtChiC, sitting drop vapor diffusion method, mixing of 0.001 ml of 5 mg/ml protein in water with 0.001 ml of reservoir solution containing 100 mM trisodium citrate, pH 5.6, and 20% PEG 10000, 20°C, 5 months, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q0WT03	gene chiC	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	quantitative real-time PCR expression analysis of gene chiC	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycol chitin + H2O	-	Arabidopsis thaliana	?	-	?
additional information	recombinant AtChiC hydrolyzes N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates, mode of action toward the oligosaccharide substrates, overview. The enzyme catalyzes transglycosylation reaction in a low efficiency	Arabidopsis thaliana	?	-	?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O	the enzyme hydrolyzes from the non-reducing end	Arabidopsis thaliana	N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose	equal amounts of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose and a lesser amount of N,N',N''-triacetylchitotriose are produced in the early stage of the reaction	?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O	the enzyme hydrolyzed N,N',N'',N''',N''''-pentaacetylchitopentaose from the non-reducing end, docking simulation, detailed overview	Arabidopsis thaliana	N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose	-	?

Subunits

Subunits	Comment	Organism
More	AtChiC adopts an (beta/alpha)8 fold with a small insertion domain composed of an alpha-helix and a five-stranded beta-sheet, secondary structure comparison, overview	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtChiC	-	Arabidopsis thaliana
class V chitinase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	40	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	assay at	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	gene chiC is induced by the plant stress-related hormones abscisic acid and jasmonic acid and by the stress resulting from the elicitor flagellin, NaCl, and osmosis	up

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Release 2023.1 (January 2023)