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Literature summary for 3.2.1.14 extracted from

  • Christodoulou, E.; Duffner, F.; Vorgias, C.E.
    Overexpression, purification, and characterization of a thermostable chitinase (Chi40) from Streptomyces thermoviolaceus OPC-520 (2001), Protein Expr. Purif., 23, 97-105.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli in cytosolic and secreted forms Streptomyces thermoviolaceus

Organism

Organism UniProt Comment Textmining
Streptomyces thermoviolaceus
-
 OPC-520
-
Streptomyces thermoviolaceus OPC-520
-
 OPC-520
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces thermoviolaceus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
 activity of secreted enzyme after Q-high performance column chromatography purification step Streptomyces thermoviolaceus
151
-
 activity of cytosolic enzyme after Q-high performance column chromatography purification step Streptomyces thermoviolaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
-
 Streptomyces thermoviolaceus N,N'-diacetylchitobiose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
-
 Streptomyces thermoviolaceus OPC-520 N,N'-diacetylchitobiose + p-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
Chi40
-
 Streptomyces thermoviolaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65 secreted form Streptomyces thermoviolaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 secreted form Streptomyces thermoviolaceus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces thermoviolaceus Phast gel electrofocusing
-
 3.5