Cloned (Comment) | Organism |
---|---|
gene chiC, expression of ChiC in Escherichia coli strain BL21(DE3) periplasm | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH dependence of ChiC kinetics, overview | Serratia marcescens | |
0.0042 | - |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside | pH 6.5, isozyme ChiA | Serratia marcescens | |
0.0068 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside | pH 6.5, isozyme ChiB | Serratia marcescens | |
0.0798 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside | pH 6.5, isozyme ChiC | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | Q700B8 | genes chiA, chiB, and chiC encoding isozymes BJL200-ChiA, BJL200-ChiB, and BJL200-ChiC, respectively | - |
Serratia marcescens BJL200 | Q700B8 | genes chiA, chiB, and chiC encoding isozymes BJL200-ChiA, BJL200-ChiB, and BJL200-ChiC, respectively | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme ChiC from Escherichia coli strain BL21(DE3) periplasm in a one step procedure using hydrophobic interaction chromatography | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Serratia marcescens |
0.0123 | - |
purified recombinant ChiC | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O | BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate | Serratia marcescens | ? | - |
? | |
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O | BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate | Serratia marcescens BJL200 | ? | - |
? | |
CM-chitin-RBV + H2O | BJL200-ChiC1 is 10-15times faster than BJL200-ChiB and BJL200-ChiA in degrading the polymeric substrate CM-chitin-RBV | Serratia marcescens | ? | - |
? | |
CM-chitin-RBV + H2O | BJL200-ChiC1 is 10-15times faster than BJL200-ChiB and BJL200-ChiA in degrading the polymeric substrate CM-chitin-RBV | Serratia marcescens BJL200 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BJL200-ChiC1 | - |
Serratia marcescens |
chitinase C1 | - |
Serratia marcescens |
More | BJL200-ChiC1 belongs to the family 18 chitinases | Serratia marcescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside | pH 6.5, isozyme ChiC | Serratia marcescens | |
56.8 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside | pH 6.5, isozyme ChiB | Serratia marcescens | |
67.4 | - |
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside | pH 6.5, isozyme ChiA | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.9 | - |
with substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside, isozyme ChiC | Serratia marcescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH dependence of ChiC kinetics, overview | Serratia marcescens |