Literature summary for 3.2.1.14 extracted from

Yang, J.; Gan, Z.; Lou, Z.; Tao, N.; Mi, Q.; Liang, L.; Sun, Y.; Guo, Y.; Huang, X.; Zou, C.; Rao, Z.; Meng, Z.; Zhang, K.Q.
Crystal structure and mutagenesis analysis of chitinase CrChi1 from the nematophagous fungus Clonostachys rosea in complex with the inhibitor caffeine (2010), Microbiology, 156, 3566-3574.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme CrChi1 free or in complex with inhibitor caffeine, hanging drop vapour diffusion method, 0.0005 ml protein solution, containing 20 mg/ml in 20 mM Tris/HCl, pH 8.0, and 0.0005 ml reservoir solution are mixed and equilibrated against 0.2 ml reservoir solution consisting of 0.2 M ammonium dihydrogen phosphate, and 15% w/v PEG3350, for the enzyme complex addition of 0.001 ml of 1 mM caffeine solution, 16°C, X-ray diffraction structure determination and analysis at 1.8 A and 1.6 A, respectively, molecular replacement	Clonostachys rosea

Crystallization (Comment)

Organism

purified enzyme CrChi1 free or in complex with inhibitor caffeine, hanging drop vapour diffusion method, 0.0005 ml protein solution, containing 20 mg/ml in 20 mM Tris/HCl, pH 8.0, and 0.0005 ml reservoir solution are mixed and equilibrated against 0.2 ml reservoir solution consisting of 0.2 M ammonium dihydrogen phosphate, and 15% w/v PEG3350, for the enzyme complex addition of 0.001 ml of 1 mM caffeine solution, 16°C, X-ray diffraction structure determination and analysis at 1.8 A and 1.6 A, respectively, molecular replacement

Clonostachys rosea

Inhibitors

Inhibitors	Comment	Organism	Structure
Caffeine	two caffeine molecules bind to CrChi1 in subsites -1 to +1 in the substrate-binding domain, binding structure, overview	Clonostachys rosea

Organism

Organism	UniProt	Comment	Textmining
Clonostachys rosea	A9LI60	-	-

Subunits

Subunits	Comment	Organism
More	CrChi1 has the DXDXE motif at the end of strand beta5, with Glu174 as the catalytic residue in the middle of the open end of the (beta/alpha)8 barrel	Clonostachys rosea

Synonyms

Synonyms	Comment	Organism
CrChi1	-	Clonostachys rosea
More	the enzyme is a family 18 chitinase	Clonostachys rosea

General Information

General Information	Comment	Organism
physiological function	chitinases are a group of enzymes capable of hydrolysing the bta-(1,4)-glycosidic bonds of chitin, an essential component of the fungal cell wall, the shells of nematode eggs, and arthropod exoskeletons	Clonostachys rosea