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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of an extracellular antifungal chitinase from Penicillium ochrochloron MTCC 517 and its application in protoplast formation (2013), Process Biochem., 48, 176-183.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 13% inhibition at 10 mM, no effect at 5 mM | Penicillium ochrochloron |  |
| Hg2+ | complete inhibition | Penicillium ochrochloron | |
| K+ | complete inhibition | Penicillium ochrochloron | |
| NH4+ | complete inhibition | Penicillium ochrochloron | |
| Zn2+ | 80% inhibition at 5 mM, complete inhibition at 10 mM; complete inhibition | Penicillium ochrochloron | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 1.3 mg/ml at pH 7.0, 40°C, with colloidal chitin | Penicillium ochrochloron |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium ochrochloron | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on enzyme activity by Ca2+ and Mg2+ at 5-10 mM | Penicillium ochrochloron |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, SDS-PAGE | Penicillium ochrochloron |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium ochrochloron | - |
- |
- |
| Penicillium ochrochloron MTCC 517 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 6.92fold by organic solvent precipitation and anion exchange chromatography | Penicillium ochrochloron |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2360 | - |
purified enzyme, pH 7.0, 40°C, substrate colloidal chitin | Penicillium ochrochloron |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Penicillium ochrochloron | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| ball-milled chitin + H2O | - |
Penicillium ochrochloron | ? | - |
? | |
| chitosan + H2O | low activity | Penicillium ochrochloron | ? | - |
? | |
| chitosan + H2O | low activity | Penicillium ochrochloron MTCC 517 | ? | - |
? | |
| chitotriose + H2O | - |
Penicillium ochrochloron | ? | - |
? | |
| chitotriose + H2O | - |
Penicillium ochrochloron MTCC 517 | ? | - |
? | |
| colloidal chitin + H2O | best substrate | Penicillium ochrochloron | N-acetyl-beta-D-glucosamine + ? | the main product after hydrolysis of colloidal chitin | ? | |
| glycol chitin + H2O | low activity | Penicillium ochrochloron | ? | - |
? | |
| glycol chitin + H2O | low activity | Penicillium ochrochloron MTCC 517 | ? | - |
? | |
| glycol chitosan + H2O | very low activity | Penicillium ochrochloron | ? | - |
? | |
| glycol chitosan + H2O | very low activity | Penicillium ochrochloron MTCC 517 | ? | - |
? | |
| additional information | exoenzyme, no activity with cellulose and carboxymethyl cellulose | Penicillium ochrochloron | ? | - |
? | |
| additional information | exoenzyme, no activity with cellulose and carboxymethyl cellulose | Penicillium ochrochloron MTCC 517 | ? | - |
? | |
| N,N'-diacetylchitobiose + H2O | high activity | Penicillium ochrochloron | 2 acetyl-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, SDS-PAGE | Penicillium ochrochloron |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Penicillium ochrochloron |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | over 60% of maximal activity within this range, 20% at 60, profile overview | Penicillium ochrochloron |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | purified enzyme, pH 7.0, over 50% activity remaining | Penicillium ochrochloron |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.37 | - |
colloidal chitin | pH 7.0, 40°C | Penicillium ochrochloron |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Penicillium ochrochloron |
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