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Literature summary for 3.2.1.14 extracted from

  • Sakai, K.; Yokota, A.; Kurokawa, H.; Wakayama, M.; Moriguchi, M.
    Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost (1998), Appl. Environ. Microbiol., 64, 3397-3402.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Bacillus sp. (in: Bacteria)
diacetylchitobiose
-
 Bacillus sp. (in: Bacteria)
Hg2+
-
 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ all isoenzymes stimulated by Ca2+ Bacillus sp. (in: Bacteria)
Mg2+ all isoenzymes stimulated by Mg2+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 isoenzyme III, gel filtration Bacillus sp. (in: Bacteria)
44000
-
 isoenzyme II, gel filtration Bacillus sp. (in: Bacteria)
53000
-
 isoenzyme III, SDS-PAGE Bacillus sp. (in: Bacteria)
62000
-
 isoenzyme II, SDS-PAGE Bacillus sp. (in: Bacteria)
68000
-
 isoenzyme I, gel filtration Bacillus sp. (in: Bacteria)
71000
-
 isoenzyme I, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) MH-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme I, 20fold, isoenzyme II 18fold, isoenzyme II 24fold Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.472
-
 isoenzyme II Bacillus sp. (in: Bacteria)
0.547
-
 isoenzyme I Bacillus sp. (in: Bacteria)
0.641
-
 isoenzyme III Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal chitin Bacillus sp. (in: Bacteria) N,N-diacetylchitobiose + N-acetylglucosamine chitobiose and chitotriose main products ?
chitin + H2O colloidal chitin Bacillus sp. (in: Bacteria) MH-1 N,N-diacetylchitobiose + N-acetylglucosamine chitobiose and chitotriose main products ?
chitohexaose + H2O
-
 Bacillus sp. (in: Bacteria) chitobiose + chitotriose
-
 ?
chitohexaose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 chitobiose + chitotriose
-
 ?
chitopentaose + H2O
-
 Bacillus sp. (in: Bacteria) chitobiose + chitotriose
-
 ?
chitopentaose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 chitobiose + chitotriose
-
 ?
chitotetraose + H2O
-
 Bacillus sp. (in: Bacteria) N-acetylglucosamine + chitobiose + chitotriose
-
 ?
chitotetraose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 N-acetylglucosamine + chitobiose + chitotriose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Bacillus sp. (in: Bacteria) N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Bacillus sp. (in: Bacteria) MH-1 N-acetylglucosamine + N,N'-diacetylchitobiose
-
 ?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Bacillus sp. (in: Bacteria) N,N'-diacetylchitobiose + p-nitrophenol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 isoenzyme II Bacillus sp. (in: Bacteria)
75
-
 isoenzyme I and II Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 isoenzyme II, completely inactivated Bacillus sp. (in: Bacteria)
85
-
 isoenzyme III, completely inactivated Bacillus sp. (in: Bacteria)
90
-
 isoenzyme I, completely inactivated Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 isoenzyme II Bacillus sp. (in: Bacteria)
5.5
-
 isoenzyme III Bacillus sp. (in: Bacteria)
6.5
-
 isoenzyme I Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4 9 isoenzyme III Bacillus sp. (in: Bacteria)
4.5 9 isoenzyme II Bacillus sp. (in: Bacteria)
6 9 isoenzyme I Bacillus sp. (in: Bacteria)