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Literature summary for 3.2.1.14 extracted from

  • Park, J.N.; Han, C.P.; Lee, D.J.; Cheon, S.A.; Kang, H.A.
    Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica (2014), J. Microbiol., 52, 284-291.
    View publication on PubMed
Search for more literature for 3.2.1.14

Application

Application Comment Organism
analysis isozyme YlCts1p can be used as a good model for studying O-glycosylation in the dimorphic yeast Yersinia lipolytica Yarrowia lipolytica

Cloned(Commentary)

Cloned (Comment) Organism
gene YlCTS1, DNA and amino acid sequence determination, analysis and comparison Yarrowia lipolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is a secreted glycoprotein modified with intensive O-mannosylation Yarrowia lipolytica
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Yarrowia lipolytica
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
 gene YlCTS1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the isozyme YlCts1p is modified only by O-mannosylation without N-glycosylation, deglycosylation by jack bean alpha-mannosidase digestion but not by PNGase F treatment Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Yarrowia lipolytica 4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
-
 ?
chitin + H2O
-
 Yarrowia lipolytica ?
-
 ?

Synonyms

Synonyms Comment Organism
endochitinase
-
 Yarrowia lipolytica
YlCts1p
-
 Yarrowia lipolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Yarrowia lipolytica

General Information

General Information Comment Organism
evolution the enzyme is a glycoside hydrolase family 18 chitinase Yarrowia lipolytica
malfunction isozyme YlCts1p deletion results in remarkably reduced extracellular endochitinase activity in the culture supernatant of Yersinia lipolytica and enhanced cell aggregation Yarrowia lipolytica
additional information isozyme YlCts1p contains an N-terminal secretion signal peptide, a long C-terminal Ser/Thr-rich domain, and a chitin-binding domain Yarrowia lipolytica
physiological function chitinase cleaves the beta-1,4-linkage between N-acetyl-D-glucosamine residues in the linear homopolymer chitin, which is an essential component of yeast and fungal cell walls Yarrowia lipolytica