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Literature summary for 3.2.1.14 extracted from

  • Patil, D.N.; Datta, M.; Chaudhary, A.; Tomar, S.; Sharma, A.K.; Kumar, P.
    Isolation, purification, crystallization and preliminary crystallographic studies of chitinase from tamarind (Tamarindus indica) seeds (2009), Acta Crystallogr. Sect. F, 65, 343-345.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
14 mg/ml purified chitinase in 100 mM Tris, pH 7.4, sitting drop vapour diffusion method, mixing of 0.002 ml protein solution with the same volume of reservoir solution containing 100 mM MES pH 6.0, 10% w/v PEG 4000, at 20°C, X-ray diffraction structure determination and analysis at 2.6 A resolution Tamarindus indica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Tamarindus indica

Organism

Organism UniProt Comment Textmining
Tamarindus indica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chitinase is purified to homogeneity by a cation and anion exchange chromatography Tamarindus indica

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Tamarindus indica
-

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Tamarindus indica

Synonyms

Synonyms Comment Organism
endochitinase
-
 Tamarindus indica
More the enzyme belongs to the acidic class III chitinase family Tamarindus indica