Cloned (Comment) | Organism |
---|---|
gene CHIA, expression of His-tagged AMCase in CHO cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetics of recombinant AMCase, steady-state kinetics,kinetic mechanism | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | ionic strength modulates substrate cleavage pattern and activity of AMCase with fluorogenic substrates, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Homo sapiens | - |
? | - |
r | |
additional information | Homo sapiens | AMCase is important in cytokine Th2-mediated inflammation and is implicated in asthma and allergic disease, inhibition of AMCase results in increased airway inflammation and airway hyperresponsiveness, the enzyme's transglycosylation activity probably is a mechanism in vivo for feedback regulation of the chitinase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BZP6 | precursor; gene CHIA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AMCase from CHO cells by nickel affinity and hydrophobic interaction chromatography, and gel filtration | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chitopentaose + H2O = chitobiose + chitotriose | catalytic reaction mechanism involving Glu119, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GlcNAc)6 + H2O | the enzyme prefers the beta-anomer of chito-oligosaccharides as substrate, cleavage of beta-glycosidic linkages from the non-reducing end primarily by disaccharide units | Homo sapiens | (GlcNAc)2 + (GlcNAc)3 + (GlcNAc)4 | product analysis | r | |
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O | - |
Homo sapiens | 4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose | product analysis | ? | |
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O | - |
Homo sapiens | 4-methylumbelliferol + beta-N,N'-diacetylchitobiose | product analysis | ? | |
chitin + H2O | - |
Homo sapiens | ? | - |
r | |
additional information | AMCase is important in cytokine Th2-mediated inflammation and is implicated in asthma and allergic disease, inhibition of AMCase results in increased airway inflammation and airway hyperresponsiveness, the enzyme's transglycosylation activity probably is a mechanism in vivo for feedback regulation of the chitinase activity | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, overview, AMCase also shows low intrinsic transglycosylation activity, product analysis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acidic mammalian chitinase | - |
Homo sapiens |
AMCase | - |
Homo sapiens |
More | AMCase is a glyco_18_domain-containing protein and a member of glycoside hydrolase family 18 | Homo sapiens |
TSA1902 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | recombinant AMCAse | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | recombinant AMCase is inactive below pH 2.0 and above pH 8.0 | Homo sapiens |