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Literature summary for 3.2.1.14 extracted from
- Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor A3(2) (2006), Biosci. Biotechnol. Biochem., 70, 988-998.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 13 genes, DNA and amino asequence determination and analysis, expression in Escherichia coli strains JM109 and BL21(DE3), sequence and domain structure comparison, overview | Streptomyces coelicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Streptomyces coelicolor | - |
? | - |
? |  |
| chitin + H2O | Streptomyces coelicolor A3(2) | - |
? | - |
? | |
| additional information | Streptomyces coelicolor | the enzyme plays a role in pathogen defense | ? | - |
? | |
| additional information | Streptomyces coelicolor A3(2) | the enzyme plays a role in pathogen defense | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
13 genes encoding 11 enzyme from chitinase family 18 and 19, overview | - |
| Streptomyces coelicolor A3(2) | - |
13 genes encoding 11 enzyme from chitinase family 18 and 19, overview | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozymes Chi18bA, Chi19F, Chi18aC and Chi18aD from Escherichia coli strain BL21(DE3) by hydroxyapatite chromatography and dialysis, recombinant isozymes Chi18bA, Chi19F from Escherichia coli strain BL21(DE3) by chitin affinity chromatography and dialysis, all enzyme are lyophilized | Streptomyces coelicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of recombinant isozymes | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Streptomyces coelicolor | ? | - |
? | |
| chitin + H2O | coloidal chitin and glycol chitin prepared from commercial powdered chitin, prawn shell chitin, crystalline beta-chitin, soluble chitin | Streptomyces coelicolor | ? | - |
? | |
| chitin + H2O | - |
Streptomyces coelicolor A3(2) | ? | - |
? | |
| chitin + H2O | coloidal chitin and glycol chitin prepared from commercial powdered chitin, prawn shell chitin, crystalline beta-chitin, soluble chitin | Streptomyces coelicolor A3(2) | ? | - |
? | |
| chitosan 7B + H2O | 70% deacetylated | Streptomyces coelicolor | ? | - |
? | |
| chitosan 7B + H2O | 70% deacetylated | Streptomyces coelicolor A3(2) | ? | - |
? | |
| additional information | the enzyme plays a role in pathogen defense | Streptomyces coelicolor | ? | - |
? | |
| additional information | chitin substrate specificities of recombinant isozymes, overview | Streptomyces coelicolor | ? | - |
? | |
| additional information | the enzyme plays a role in pathogen defense | Streptomyces coelicolor A3(2) | ? | - |
? | |
| additional information | chitin substrate specificities of recombinant isozymes, overview | Streptomyces coelicolor A3(2) | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzymes belong to the chitinase families 18, Chi18aC and Chi18aD, and family 19, isozymes Chi18bA and Chi19F | Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant isozymes Chi18bA and Chi19F | Streptomyces coelicolor |
| 60 | - |
recombinant isozymes Chi18aC and Chi18aD | Streptomyces coelicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | temperature profile of recombinant isozymes, overview | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the pH optimum depends strongly on the chitin substrate used | Streptomyces coelicolor |
| 2 | - |
recombinant isozyme Chi18aC | Streptomyces coelicolor |
| 3 | 4 | recombinant isozyme Chi18aD | Streptomyces coelicolor |
| 4 | 8 | recombinant isozyme Chi19F, dependent on substrate | Streptomyces coelicolor |
| 4 | 6 | recombinant isozyme Chi18bA, dependent on substrate | Streptomyces coelicolor |
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