Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Antheraea pernyi |
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Ostrinia furnacalis |
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Agrius convolvuli |
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Mythimna separata |
DNA and amino acid sequence determination and analysis, phylogenetic analysis | Samia cynthia |
gene Chi-h, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged wild-type and mutant Chi-h in Spodoptera frugiperda Sf9 cells using the Autographa californica nucleopolyhedrovirus for transfection constructed in a Bac-to-Bac system | Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
D306N | site-directed mutagenesis using different sets of primers, the mutant enzyme shows reduced activity and high pH sensitivity compared to the wild-type enzyme | Bombyx mori |
D306N/D310Q | site-directed mutagenesis using different sets of primers, inactive mutant | Bombyx mori |
D310Q | site-directed mutagenesis using different sets of primers, inactive mutant | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bombyx mori | Chi-h plays a role in the chitin degradation during the molting process | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrius convolvuli | Q4AE58 | - |
- |
Antheraea pernyi | Q4AE61 | - |
- |
Bombyx mori | Q869E2 | gene Chi-h encodes an exochitinase, the organism also contains an endochitinase | - |
Mythimna separata | Q4AE57 | i.e. Mythimna separata | - |
Ostrinia furnacalis | Q4AE59 | - |
- |
Samia cynthia | Q4AE60 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant Chi-h from Sf9 insect cells by metal affinity chromatography and dialysis | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O | - |
Bombyx mori | 4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose | - |
? | |
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O | - |
Bombyx mori | 4-methylumbelliferol + beta-N,N'-diacetylchitobiose | - |
? | |
4-methylumbelliferyl beta-N-acetyl-D-glucosamine + H2O | - |
Bombyx mori | 4-methylumbelliferol + beta-N-acetyl-D-glucosamine | - |
? | |
chitin oligosaccharides + H2O | glycol chitin assay method, (GlcNAc)1-6 | Bombyx mori | N-acetyl glucosamine | - |
? | |
additional information | Chi-h plays a role in the chitin degradation during the molting process | Bombyx mori | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacterial-type chitinase | - |
Bombyx mori |
Chi-h | - |
Antheraea pernyi |
Chi-h | - |
Ostrinia furnacalis |
Chi-h | - |
Agrius convolvuli |
Chi-h | - |
Mythimna separata |
Chi-h | - |
Bombyx mori |
Chi-h | - |
Samia cynthia |
exochitinase | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, with fluorescent substrates | Bombyx mori |
37 | - |
assay at, with chitin substrates | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
exochitinase mutant D306N | Bombyx mori |
6.2 | - |
wild-type exochitinase | Bombyx mori |