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Literature summary for 3.2.1.14 extracted from

  • Duivenvoorden, B.A.; Ghauharali, K.; Scheij, S.; Boot, R.G.; Aerts, J.M.; van der Marel, G.A.; Overkleeft, H.S.; Codee, J.D.
    Design and synthesis of 4-O-alkyl-chitobiosyl-4-methylumbelliferone as human chitinase fluorogenic substrates (2014), Carbohydr. Res., 399, 26-37.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression in BHK cells and secretion to the culture medium Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from BHK cell culture supernatant by ultrafiltration and concanavalin A affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
macrophage the enzyme is expressed and secreted in high quantities by lipid-laden tissue macrophages in Gaucher patients Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetamido-2-deoxy-4-O-(2-acetamido-2-deoxy-4-O-methyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens ?
-
 ?
2-acetamido-2-deoxy-4-O-(2-acetamido-4-O-cyclohexylmethyl-2-deoxy-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens ?
-
 ?
2-acetamido-2-deoxy-4-O-(2-deoxy-2-acetamido-4-O-isopropyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens ?
-
 ?
additional information synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, overview. The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed, unmodified chitobiosyl-4-methylumbelliferone undergoes transglycosylation. No activity with N-acetylglucosaminyl-4-methylumbelliferone Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
CHIT1
-
 Homo sapiens
chitotriosidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5.2 assay at, substrate-dependent Homo sapiens