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Literature summary for 3.2.1.14 extracted from

  • Fernandez-Caballero, C.; Romero, I.; Goni, O.; Escribano, M.I.; Merodio, C.; Sanchez-Ballesta, M.T.
    Characterization of an antifungal and cryoprotective class I chitinase from table grape berries (Vitis vinifera cv. Cardinal) (2009), J. Agric. Food Chem., 57, 8893-8900.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vitis vinifera

Organism

Organism UniProt Comment Textmining
Vitis vinifera Q546P8 cv. Cardinal
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, from inclusion bodies Vitis vinifera

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Vitis vinifera
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.36
-
 37°C Vitis vinifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylated-chitin-remazol brilliant violet + H2O
-
 Vitis vinifera ?
-
 ?

Synonyms

Synonyms Comment Organism
Chit1b
-
 Vitis vinifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Vitis vinifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 50 more than 50% of maximum activity Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Vitis vinifera

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 more than 50% of maximum activity within Vitis vinifera

General Information

General Information Comment Organism
physiological function enzyme exhibits in vitro antifungal activity against Botrytis cinerea and is able to cryoprotect lactate dehydrogenase against freeze/thaw inactivation Vitis vinifera