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Literature summary for 3.2.1.14 extracted from

  • Chen, A.C.; Mayer, R.T.; DeLoach, J.R.
    Purification and characterization of chitinase from the stable fly, Stomoxys calcitrans (1982), Arch. Biochem. Biophys., 216, 314-321.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Stomoxys calcitrans
Cu2+
-
Stomoxys calcitrans
Fe2+
-
Stomoxys calcitrans
Hg2+
-
Stomoxys calcitrans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
33
-
chitin expressed in N-acetylglucosamine equivalents Stomoxys calcitrans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 48000, SDS-PAGE Stomoxys calcitrans

Organism

Organism UniProt Comment Textmining
Stomoxys calcitrans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Stomoxys calcitrans

Source Tissue

Source Tissue Comment Organism Textmining
pupa
-
Stomoxys calcitrans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7189
-
-
Stomoxys calcitrans

Storage Stability

Storage Stability Organism
-20°C, prolonged storage, repeated freezing and thawing, no loss of activity Stomoxys calcitrans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O [acetyl-3H]chitin Stomoxys calcitrans N,N-diacetylchitobiose + N-acetylglucosamine
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Stomoxys calcitrans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Stomoxys calcitrans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 between pH 5.5 and 7, 80% activity Stomoxys calcitrans