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Literature summary for 3.2.1.14 extracted from

  • Skujins, J.; Pukite, A.; McLaren, A.D.
    Chitinase of Streptomyces sp.: Purification and properties (1970), Enzymologia, 39, 353-370.
    View publication on PubMed
Search for more literature for 3.2.1.14

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
AlCl3 deletorious effect at 0.01 M Streptomyces sp.
Co2+ inhibiting above 1 mM Streptomyces sp.
Mg2+
-
 Streptomyces sp.
NaCl
-
 Streptomyces sp.
Zn2+ inhibiting above 1 mM Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ necessary in small amounts in order to retain enyzmatic activity during purification Streptomyces sp.
Co2+ activating below 1 mM Streptomyces sp.
Zn2+ activating below 1 mM Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 equilibrium sedimentation Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. 2B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.289
-
-
 Streptomyces sp.

Storage Stability

Storage Stability Organism
2°C, lycophilized, after purifiaction step 5, stable Streptomyces sp.
frozen, prolonged periods, after purification step 4, stable Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Streptomyces sp. N,N-diacetylchitobiose + N-acetylglucosamine N-acetylhexosamine ?
chitin + H2O
-
 Streptomyces sp. 2B N,N-diacetylchitobiose + N-acetylglucosamine N-acetylhexosamine ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 loss of all activity after 3 h Streptomyces sp.
70
-
 loss of all activity after 5 min, 80% of activity remains when dried Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 Na-phosphate buffer Streptomyces sp.
4.8
-
 Na-acetate buffer, sodium phosphate-acetate buffer Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
11
-
 inactivated above pH 11 Streptomyces sp.