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Literature summary for 3.2.1.14 extracted from

  • Li, Y.C.; Chang, C.T.; Hsiao, E.S.; Hsu, J.S.; Huang, J.W.; Tzen, J.T.
    Purification and characterization of an antifungal chitinase in jelly fig (Ficus awkeotsang) achenes (2003), Plant Cell Physiol., 44, 1162-1167.
    View publication on PubMed
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 60°C, pH 4 Ficus pumila var. awkeotsang

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Ficus pumila var. awkeotsang

Organism

Organism UniProt Comment Textmining
Ficus pumila var. awkeotsang
-
 jelly fig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ficus pumila var. awkeotsang

Source Tissue

Source Tissue Comment Organism Textmining
pericarp
-
 Ficus pumila var. awkeotsang
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
731
-
 after Sephadex G-75 purification step Ficus pumila var. awkeotsang

Storage Stability

Storage Stability Organism
4°C, at least one year Ficus pumila var. awkeotsang

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Ficus pumila var. awkeotsang ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Ficus pumila var. awkeotsang

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Ficus pumila var. awkeotsang

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 retaining activity for several hours Ficus pumila var. awkeotsang

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.089
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 60°C, pH 4 Ficus pumila var. awkeotsang

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Ficus pumila var. awkeotsang

pI Value

Organism Comment pI Value Maximum pI Value
Ficus pumila var. awkeotsang lower than pH 3.5, isoelectric focusing
-
 additional information