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Literature summary for 3.2.1.14 extracted from
- Cloning and characterization of a chitinase gene Lbchi31 from Limonium bicolor and identification of its biological activity (2009), Mol. Biol. Rep., 37, 2447-2453.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Limonium bicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | strong inhibition | Limonium bicolor |  |
| Cu2+ | strong inhibition | Limonium bicolor | |
| Mg2+ | strong inhibition | Limonium bicolor | |
| Na+ | strong inhibition | Limonium bicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimualtion | Limonium bicolor | |
| Mn2+ | maximal activity at 5 mM | Limonium bicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31700, calculated, x * 31000, SDS-PAGE | Limonium bicolor |
| 31700 | - |
x * 31700, calculated, x * 31000, SDS-PAGE | Limonium bicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limonium bicolor | B3VFX0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | cell wall chitin of Valsa sordida | Limonium bicolor | ? | - |
? | |
| additional information | enzyme efficiently degrades cell wall chitin of the phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici and Phytophthora sojae | Limonium bicolor | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31700, calculated, x * 31000, SDS-PAGE | Limonium bicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Chi31 | - |
Limonium bicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Limonium bicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | stable for at least 30 min | Limonium bicolor |
| 65 | - |
above, rapid loss of activity | Limonium bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Limonium bicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | - |
Limonium bicolor |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Limonium bicolor | calculated | - |
5.5 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme efficiently degrades cell wall chitin of the phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici and Phytophthora sojae | Limonium bicolor |
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