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Literature summary for 3.2.1.14 extracted from

  • Chen, Y.T.; Hsu, L.H.; Huang, I.P.; Tsai, T.C.; Lee, G.C.; Shaw, J.F.
    Gene cloning and characterization of a novel recombinant antifungal chitinase from papaya (Carica papaya) (2007), J. Agric. Food Chem., 55, 714-722.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Carica papaya

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 10 mM, 16.3% loss of activity Carica papaya
Cu2+ 10 mM, 60% loss of activity Carica papaya
EDTA 10 mM, 6.7% loss of activity Carica papaya
Fe3+ 10 mM, 29.3% loss of activity Carica papaya
Hg2+ 10 mM, complete loss of activity Carica papaya
K+ 10 mM, 7.7% loss of activity Carica papaya
Mn2+ 10 mM, 64.7% loss of activity Carica papaya
Zn2+ 10 mM, 62.0% loss of activity Carica papaya

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE Carica papaya
26200
-
 x * 26200, calculated from sequence Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
 L. cv. Tainong 2
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Carica papaya
-

Storage Stability

Storage Stability Organism
30°C, 3 weeks, 36% loss of activity Carica papaya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycol chitin + H2O exochitinase activity that yields p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose, and then when the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is accumulated, the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is further cleaved into N,N'-diacetylchitobiose and GlcNAc Carica papaya N,N'-diacetylchitobiose + GlcNAc
-
 ?
additional information recombinant CpCHI shows antibacterial activity in which 50% of Escherichia coli is inhibited by a 0.0025 mM concentration of the enzyme. Antifungal activity: the spore germination of Alternaria brassicicola can be completely inhibited by a 76 nM level of recombinant CpCHI Carica papaya ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Carica papaya
? x * 26200, calculated from sequence Carica papaya

Synonyms

Synonyms Comment Organism
CpCHI
-
 Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 purified recombinant enzyme Carica papaya

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70 10°C: 85% of maximal activity, 70°C: about 60% or maximal activity Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 3 weeks, 36% loss of activity Carica papaya
37
-
 3 weeks, less than 50% loss of activity Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 9 broad optimal pH-range Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
3 10 pH 4: about 50% of maximal activity, pH 10: about 65% of maximal activity Carica papaya

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 3 h, about 70% loss of activity Carica papaya
4
-
 3 h, about 30% loss of activity Carica papaya
5 9 3 h, no loss of activity Carica papaya
10
-
 3 h, about 35% loss of activity Carica papaya

pI Value

Organism Comment pI Value Maximum pI Value
Carica papaya calculated from sequence
-
 6.32