Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hevea brasiliensis |
Crystallization (Comment) | Organism |
---|---|
grown from 1.1-1.4 M ammonium sulfate or PEG3350 solutions, pH 7 | Hevea brasiliensis |
Protein Variants | Comment | Organism |
---|---|---|
D125A | nearly complete loss in activity | Hevea brasiliensis |
D125N | twofold lower turnover and twofold higher KM than wild-type, also active at higher pH-values | Hevea brasiliensis |
E127A | nearly complete loss in activity | Hevea brasiliensis |
Y183F | lower turnover than wild-type | Hevea brasiliensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, Y183F | Hevea brasiliensis | |
0.016 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, D125N | Hevea brasiliensis | |
0.02 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, recombinant wild-type | Hevea brasiliensis | |
0.028 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, purified wild-type | Hevea brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitopentaose + H2O | - |
Hevea brasiliensis | ? | - |
? | |
colloidal chitin + H2O | - |
Hevea brasiliensis | ? | - |
? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O | - |
Hevea brasiliensis | 5 N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hevamine | - |
Hevea brasiliensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, Y183F | Hevea brasiliensis | |
0.28 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, D125N | Hevea brasiliensis | |
0.61 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, recombinant wild-type | Hevea brasiliensis | |
0.77 | - |
N,N',N'',N''',N''''-pentaacetylchitopentaose | 30°C, pH 4.2, purified wild-type | Hevea brasiliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
with chitopentaose as substrate | Hevea brasiliensis |