EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.2.1.14 | -999 |
- |
colloidal chitin |
kcat/Km value 0.002 ml/mg/s, pH 4.5, 50°C |
742728 |
| 3.2.1.14 | -999 |
- |
colloidal chitin |
Km value 4.6 mg/ml, pH 5, 55°C |
742187 |
| 3.2.1.14 | -999 |
- |
glycol chitin |
kcat/Km value 0.010 ml/mg/s, pH 4.5, 50°C |
742728 |
| 3.2.1.14 | -999 |
- |
more |
the kcat/Km of the (beta-1,4-GlcNAc)n hydrolysis increases with an increase in the degree of polymerization of the substrates |
717645 |
| 3.2.1.14 | 0.0017 |
- |
4-nitrophenyl chitobioside |
pH 4.8, 50°C, mutant enzyme W664A |
724957 |
| 3.2.1.14 | 0.09 |
- |
4-methylumbelliferyl chitobioside |
pH 5.0, 25°C |
708581 |
| 3.2.1.14 | 0.13 |
- |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc |
mutant W275G, 37°C |
709253 |
| 3.2.1.14 | 0.25 |
- |
4-nitrophenyl chitobioside |
pH 4.8, 50°C, mutant enzyme D524A |
724957 |
| 3.2.1.14 | 0.25 |
- |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc |
mutant W275G, 37°C |
709253 |
| 3.2.1.14 | 0.38 |
- |
4-nitrophenyl chitobioside |
pH 4.8, 50°C, mutant enzyme E526Q |
724957 |
