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Literature summary for 3.2.1.14 extracted from

  • Lee, Y.S.; Park, I.H.; Yoo, J.S.; Chung, S.Y.; Lee, Y.C.; Cho, Y.S.; Ahn, S.C.; Kim, C.M.; Choi, Y.L.
    Cloning, purification, and characterization of chitinase from Bacillus sp. DAU101 (2007), Biores. Technol., 98, 2734-2741.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, 95% inhibition Bacillus sp. (in: Bacteria)
EDTA 10 mM, 15% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
Zn2+ 5 mM, complete inhibition Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM, 1.36fold increase in activity Bacillus sp. (in: Bacteria)
Ca2+ 5 mM, 1.25fold increase in activity Bacillus sp. (in: Bacteria)
Co2+ 5 mM, 1.43fold increase in activity Bacillus sp. (in: Bacteria)
Li+ 5 mM, 1.2fold increase in activity Bacillus sp. (in: Bacteria)
Mg2+ 5 mM, 1.24fold increase in activity Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A0EQW8
-
-
Bacillus sp. (in: Bacteria) DAU101 A0EQW8
-
-

Purification (Commentary)

Purification (Comment) Organism
GST-fusion purification system Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
Bacillus sp. (in: Bacteria) N,N'-diacetylchitobiose + ?
-
?
chitin + H2O
-
Bacillus sp. (in: Bacteria) DAU101 N,N'-diacetylchitobiose + ?
-
?
chitosan + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
chitosan + H2O
-
Bacillus sp. (in: Bacteria) DAU101 ?
-
?
additional information the enzyme can not hydrolyze glycol chitin or glycol chitosan Bacillus sp. (in: Bacteria) ?
-
?
additional information the enzyme can not hydrolyze glycol chitin or glycol chitosan Bacillus sp. (in: Bacteria) DAU101 ?
-
?
N,N'-diacetylchitobiose + H2O
-
Bacillus sp. (in: Bacteria) N-acetylglucosamine
-
?
N,N'-diacetylchitobiose + H2O
-
Bacillus sp. (in: Bacteria) DAU101 N-acetylglucosamine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Bacillus sp. (in: Bacteria)