Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Andronopoulou, E.; Vorgias, C.E.
    Purification and characterization of a new hyperthermostable, allosamidin-insensitive and denaturation-resistant chitinase from the hyperthermophilic archaeon Thermococcus chitonophagus (2003), Extremophiles, 7, 43-53.
    View publication on PubMed

General Stability

General Stability Organism
40% activity in presence of 1 M NaCl Thermococcus chitonophagus
in the presence of 3 M guanidine hydrochloride no activity left Thermococcus chitonophagus
in the presence of 3 M urea over 50% of initial activity left Thermococcus chitonophagus

Inhibitors

Inhibitors Comment Organism Structure
dimethyl sulfoxide
-
Thermococcus chitonophagus
guanidine hydrochloride
-
Thermococcus chitonophagus
polyethyleneglycol 400
-
Thermococcus chitonophagus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
p-nitrophenol-beta-D-N,N'-diacetylchitobioside 70°C, pH 7 Thermococcus chitonophagus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with the outer side of cell membrane Thermococcus chitonophagus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
1 * 70000, SDS-PAGE Thermococcus chitonophagus

Organism

Organism UniProt Comment Textmining
Thermococcus chitonophagus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Thermococcus chitonophagus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
p-nitrophenol-beta-D-lactopyranoside or p-nitrophenol-beta-D-galactopyranoside as substrate Thermococcus chitonophagus
0.35
-
p-nitrophenol-beta-D-cellobioside as substrate Thermococcus chitonophagus
0.63
-
p-nitrophenol-beta-D-mannopyranoside as substrate Thermococcus chitonophagus
0.96
-
p-nitrophenol-beta-D-maltoside as substrate Thermococcus chitonophagus
1.26
-
p-nitrophenol-beta-D-glucopyranoside as substrate Thermococcus chitonophagus
2.07
-
p-nitrophenol-beta-D-N,N'-diacetylchitobioside as substrate as substrate Thermococcus chitonophagus
230
-
chitosan as substrate Thermococcus chitonophagus
270
-
cellulose as substrate Thermococcus chitonophagus
310
-
chitin as substrate Thermococcus chitonophagus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
Thermococcus chitonophagus 4-nitrophenol + beta-D-cellobiose
-
?
cellulose + H2O
-
Thermococcus chitonophagus ?
-
?
chitin + H2O
-
Thermococcus chitonophagus chitobiose + ?
-
?
chitosan + H2O
-
Thermococcus chitonophagus ?
-
?
N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose + H2O
-
Thermococcus chitonophagus ?
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
Thermococcus chitonophagus ?
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
Thermococcus chitonophagus ?
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
Thermococcus chitonophagus ?
-
?
N,N',N''-triacetylchitotriose + H2O
-
Thermococcus chitonophagus ?
-
?
N,N'-diacetylchitobiose + H2O
-
Thermococcus chitonophagus N-acetyl-D-glucosamine
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
-
Thermococcus chitonophagus D-cellobiose + p-nitrophenol
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
-
Thermococcus chitonophagus D-galactopyranose + p-nitrophenol
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
Thermococcus chitonophagus D-glucopyranose + p-nitrophenol
-
?
p-nitrophenyl-beta-D-lactopyranoside + H2O
-
Thermococcus chitonophagus D-lactopyranose + p-nitrophenol
-
?
p-nitrophenyl-beta-D-maltoside + H2O
-
Thermococcus chitonophagus D-maltose + p-nitrophenol
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O
-
Thermococcus chitonophagus D-mannopyranose + p-nitrophenol
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
-
Thermococcus chitonophagus N,N'-diacetylchitobiose + p-nitrophenol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Thermococcus chitonophagus

Synonyms

Synonyms Comment Organism
Chi70
-
Thermococcus chitonophagus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermococcus chitonophagus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90 100 over 50% activity after 48 h Thermococcus chitonophagus
110
-
half-life of 4 h Thermococcus chitonophagus
120
-
half-life of 1 h Thermococcus chitonophagus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
chitosan
-
Thermococcus chitonophagus
0.003
-
cellulose
-
Thermococcus chitonophagus
0.005
-
chitin
-
Thermococcus chitonophagus
0.017
-
p-nitrophenol-beta-D-galactopyranoside
-
Thermococcus chitonophagus
0.025
-
p-nitrophenol-beta-D-lactopyranoside
-
Thermococcus chitonophagus
0.032
-
p-nitrophenol-beta-D-mannopyranoside
-
Thermococcus chitonophagus
0.04
-
4-nitrophenol-beta-D-cellobioside
-
Thermococcus chitonophagus
0.07
-
p-nitrophenol-beta-D-maltoside
-
Thermococcus chitonophagus
0.095
-
p-nitrophenol-beta-D-glucopyranoside
-
Thermococcus chitonophagus
0.383
-
p-nitrophenol-beta-D-N,N'-diacetylchitobioside 70°C, pH 7 Thermococcus chitonophagus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermococcus chitonophagus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5
-
Thermococcus chitonophagus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 over 50% activity after 24 h Thermococcus chitonophagus

pI Value

Organism Comment pI Value Maximum pI Value
Thermococcus chitonophagus isoelectric focusing
-
6