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3.2.1.14: chitinase

This is an abbreviated version!
For detailed information about chitinase, go to the full flat file.

Word Map on EC 3.2.1.14

Reaction

chitopentaose
+
H2O
=
chitobiose
 +
chitotriose

Synonyms

1,4-beta-poly-N-acetylglucosaminidase, AcD1ChiA, acid mammalian chitinase, acidic chitinase, acidic mammalian chitinase, active phase-associated protein I, active phase-associated protein II, AD2PF-ChiA, AgChi, alpha-chitinase, AMCase, antifreeze protein, APAP1, APAP2, APAPI, APAPII, AtChiC, B220, bacterial-type chitinase, Bbchit1, BChi14, beta-1,4-poly-N-acetyl glucosamidinase, BjCHI1, BJL200-ChiC1, CF-AG, CF-antigen, CGCHI3, CH5B, ChBDChiC, CHI, Chi-1, CHI-26, Chi-A, Chi-h, Chi1, Chi18A, Chi18B, Chi18C, Chi19, CHI2, Chi2 enzyme, Chi21, Chi22, Chi3, Chi31, Chi35, Chi40, Chi44, Chi46, Chi54, CHI60, Chi67, Chi70, Chi72, Chi78, ChiA, ChiA Nima, ChiA protein, ChiA-HD73, ChiA-Hh59, ChiA-Mt45, ChiA1, ChiA74, ChiB, ChiC, ChiCW, ChiD, ChiE, ChiEN1, ChiF, ChiF1, ChiG, ChiNCTU2, Chit, CHIT 1A, CHIT 1B, CHIT1, Chit1b, Chit33, CHIT42, Chit62, ChitA, ChitIII-1, ChitIII-2, chitinase, chitinase 1, chitinase 18C, chitinase 2, chitinase 60, chitinase 92, chitinase A, chitinase A1, chitinase B, chitinase C, chitinase C1, chitinase Chi255, chitinase F1, chitinase G, chitinase I, chitinase II, chitinase III, chitinase-A, chitinases A, chitinolytic enzyme, chitodextrinase-N-, chitotriosidase, ChiW, CHT1, Cht4, CHT7, ChtII, ChtIII, CiX1, class I chitinase, class II chitinase, class III chitinase, class IV chitinase, class V chitinase, Complement-fixation antigen, CpCHI, CrChi1, CTS1, ECH, ECH1, Ech42, ech46, ELC, endo-chitinase, endochitinase, endochitinase 1, endochitinase-1, endochitinase-2, exochitinase, GAC1, GAC2, GH18 chitinase, group I chitinase, group II chitinase, group III chitinase, hevamine, HoChiA, HoChiB, HoChiC, HschiA1, Is-chiB, LlChi18A, LpChiA, MACase, MF1 antigen, MmChi60, More, MsChi386, MsChi535, NaCHIT1, NaCHIT3, NtChitIV, NtChiV, OfChtI, OsChia1b, OsChia1c, OsChia1cDELTAChBD, OsChia1d, OsChia2a, OsChia2b, OsChia4a, OsChib1a, PF1233, PjChi-1, plant class III chitinase, PLC-A, PLC-B, PLChiA, poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase, poly-beta-glucosaminidase, PrChi-A, Ptchi19p, Ra-ChiC, RCB4, RHBC, sAMC, SCChi-1, SI-CLP, SjChi, SsChi50, stabilin-1 interacting chitinase-like protein, STK_09390, TBC-1, Tfu_0580, Tk-ChiA, TKU028 chitinase, TmChi, TSA1902, UDA, V-CHIA, VpChiA, WIN6, YlCts1p, Ym1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.14 chitinase

Cloned

Cloned on EC 3.2.1.14 - chitinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
13 genes, DNA and amino asequence determination and analysis, expression in Escherichia coli strains JM109 and BL21(DE3), sequence and domain structure comparison, overview
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a C-terminal truncated form of chiA lacking a putative chitin-binding domain, recombinant expression of the N-terminally His-tagged mutant in Escherichia coli strain BL21 (DE3)
Salmonella enterica typhimurium
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AgChi cDNA is expressed as a 46000 Da polypeptide in baculovirus-infected insect Sf9 cells and the recombinant AgChi shows activity in a chitinase enzyme assay
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C-terminal truncated enzyme, ChiCWDELTAFC, is expressed in Escherichia coli
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catalytic domain Cat2 is expressed in Escherichia coli Rosetta-gami (DE3) cells
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CHIT1, DNA and amino acid sequence determination and analysis
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chitin-binding domain ChBD of chitinase A1 is C-terminally fused to D-hydantoinase, expression of the chimeric fusion protein in Escherichia coli as soluble protein
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chitinase A, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Rosetta-gami B (DE 3), and coexpression of chitinase A with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium in which chitinase A dominates alpha1,3-glucanase
chitinase I, coexpression of chitinase I with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium
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cloning in Escherichia coli strain JM109, removal of the C-terminal VTS of 24 bp and replacement of the N-terminal signal sequence of yam chitinase with alpha-factor of the expression vector pPICZalphaA, recombinant expression in Pichia pastoris strain X-33 an secretion to the culture medium
co-expression of the class II chitinase with type I ribosome inactivating protein in Brassica juncea via Agrobacterium tumefaciens strain EHA105-mediated transformation. The expression of chitinase and type I ribosome inactivating protein from a heterologous source in Brassica juncea provide subsequent protection against Alternaria leaf spot disease, phenotype, overview
constructed chimeric genes by fusion of the deletion series of NtChitIV promoter to GFP reporter gene
detection of two adjacent genes (PF1233 and PF1234) homologous to chitinase genes of Thermococcus kodakarensis in pyrococcus furiosus. In the cultured medium of Pyrococcus furiosus no chitinase is detected. On analysis of the structural gene of Pyrococcus furiosus, it appears that one nucleotide insertion in PF1234 causes a frame shift and separates a gene. By deletion of one nucleotide in PF1234, the best match is achieved between chitinases of Thermococcus kodakarensis and Pyrococcus furiosus. An artificial recombinant chitinase is constructed, exhibiting hydrolytic activity towards colloidal and crystalline chitins at high temperatures. Expression in Escherichia coli
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DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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DNA and amino acid sequence determination and analysis, phylogenetic analysis
DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
DNA and amino acid sequence determination and analysis, sequence comparison
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enzyme expression in BHK cells and secretion to the culture medium
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expressed in Bacillus amyloliquefaciens
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expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) CodonPlus cells
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expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells
expressed in Escherichia coli Rosetta 2 cells
expressed in Hi-5 insect cells
expressed in Pichia pastoris
expressed in Pichia pastoris GS115 cells
expressed in Pichia pastoris PichiaPink Strain 1
expressed in Pichia pastoris strain GS115
expressed in tobacco
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expression analysis of two class III isozymes ChitIII-1 and ChitIII-2 in roots
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expression as GST-fusion protein in Escherichia coli
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expression in A-549 cells
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expression in Arabidopsis thaliana
expression in Bacillus thuringiensis under the control of a strong promoter and a 5'-mRNA stabilizing sequence as well as under control of the native promoter
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expression in cotton plants
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expression in Escherichia coli
expression in Escherichia coli and Pichia pastoris. The high-level production of recombinant Bbchit1 is detected in Escherichia coli expression system, however mainly located in inclusion bodies. Refolding of solubilized inclusion body proteins is achieved by dialysis. In Pichia pastoris expression system, Bbchit1 is secreted into the culture medium under the induction of methanol. The specific activity of Bbchit1 from Pichia pastoris is higher than that from Escherichia coli
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expression in Escherichia coli BL21 with a N-terminal T7 tag and a C-terminal His tag
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expression in Escherichia coli containing a N-terminal additional Met and a C-terminal His tag
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expression in Escherichia coli DH5alphaF'
expression in Escherichia coli JM109
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expression in Escherichia coli M15 (pREP4)
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expression in Escherichia coli. Chi255 is excessively degraded by the action of Escherichia coli proteases. When the chitin binding domain (CBD) is deleted from Chi255, an active form (Chi2555DELTA5) of expected size of about 60000 Da is extracted from the Escherichia coli periplasmic fraction, without the observation of any proteolytic degradation
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expression in Escherichia coli. Cytoplasmic overexpression results in inclusion body formation. Active enzyme can be recovered after refolding. Periplasmic proves to be better suited for mutagenesis purposes
expression in Hi-5 insect cells using baculovirus vectors
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expression in Nicotiana sylvestris
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expression in Nicotiana tabacum
expression in Pichia pastoris
expression of a clone named RCB4l, that encodes the N-terminal and internal amino acid sequence, in Escherichia coli
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expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli
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expression of ChiCW in Escherichia coli strain DH5alpha periplasm
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expression of ChiG in Escherichia coli
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expression of GST-tagged AMCase in Escherichia coli
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expression of His-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, method optimization, overview
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expression of N-terminally His6-tagged mature Ra-ChiC and of Ra-ChiC89–252 domain in Escherichia coli as a soluble proteins
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expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS
expression of the catalytic domain of ScCTS1 (comprising of amino acids 22 to 315) as a secreted N-terminally His-tagged fusion protein in Pichia pastoris
expression of the secreted enzyme in Pichia pastoris
expression of wild-type and mutant enzymes in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
expression of wild-type and truncation mutant enzymes in Escherichia coli BL21(DE3)
-
expression of wild-type enzyme and mutant enzymes in Escherichia coli
expression of wild-type OsChia1b and the two mutant chitinases, loop II-deletion mutant (OsChia1bDELTAloopII) and W159A mutant (OsChia1bW159A) in Escherichia coli
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expression with wheat germ cell-free system
full-length and truncated chitinases are generated in Escherichia coli
gene BiCHT1, functional overexpression in Escherichia coli, functional and expression in transgenic bromegrass cells using Agrobacterium-mediated transformation, recombinant enzymes do not show anti-freeze activity in the expression systems, overview
gene ch5B, expression in strawberry, Fragaria sp. cv. Pajaro, using the Agrobacterium tumefaciens strain LBA4404 transfection system
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gene chi, cloning in Escherichia coli strain NEB 5alpha, recombinant expression of His-tagged enzyme in Lactobacillus plantarum strain WCFS1 using inducible lactobacillal expression vectors pSIP403 and pSIP409, derived from the sakacin-P operon of Lactobacillus sakei
gene Chi-h, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged wild-type and mutant Chi-h in Spodoptera frugiperda Sf9 cells using the Autographa californica nucleopolyhedrovirus for transfection constructed in a Bac-to-Bac system
gene chi2, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris strain GS115
gene Chi3-1, expression in Pichia pastoris strain X-33
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gene chi46, DNA and amino acid sequence determination and analysis, multiple regulatory proteins are involved in wild-type Chi46 expression, expression in Escherichia coli
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gene chi46, genomic cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in Pichia pastoris strain GS115
gene chi60, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
gene chiA Nima
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gene chiA, cloning of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, heterologous expression in Pichia pastoris strain X-33
gene chiA, expression as GFP-tagged protein in Aspergillus nidulans, expression pattern analysis, overview
gene chiA, expression in Escherichia coli strain XL-1 Blue
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gene CHIA, expression of His-tagged AMCase in CHO cells
gene chiB, DNA and amino acid sequence determination and analysis, expression of His-tagged Is-chiB in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha
gene chiB337, functional expression in Streptomyces fradiae strain SU-1 using the Escherichia coli-Streptomyces shuttle vector pFIS318 for transformation of protoplasts, secretion of chitinases by the recombinant strain, subcloning in Escherichia coli strain JM109
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gene chiC, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene chiC, expression of ChiC in Escherichia coli strain BL21(DE3) periplasm
gene chiC, quantitative real-time PCR expression analysis, recombinant expression in Escherichia coli
Q0WT03
gene chiD, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene chiE, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene chiF, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene chiG, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene chiV, DNA and amino acid sequence determination and analysis, wild-type and mutant NtChiVs recombinant expression in Escherichia coli strain Tuner(DE3) pLacI. Expression of SeMet-labeled NtChiV in the methionine auxotroph Escherichia coli strain B834(DE3) pLacI
gene ChiW-SLHd, DNA and amino acid sequence determination and analysis, phylogenetic analysis, N-terminally His-tagged enzyme, the enzyme's N-terminus lacks the signal peptide and SLH domains , overexpression of active enzyme ChiW and mutants in Escherichia coli strain BL21
gene Cht-2, expression in Escherichia coli
-
gene cts1 from yeast cells, cDNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain XL1-Blue
gene LlChi18A, DNA and amino acid sequence determination and analysis, expression of the soluble His-tagged enzyme in Escherichia coli BL21(DE3)
gene MSCH, expression in Carica papaya leaves by microprojectile bombardment, using a gene gun, of embryonic calli derived from hypocotyls of the cultivar Kapoho, DNA sequence determination in transgenic lines
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gene Ptchi19, DNA and amino acid sequence determination and analysis, expression in Eschereichia coli
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gene SafchiA, DNA and amino acid sequence determination and analysis, phylogenetic tree, RT-PCR expression analysis, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), the recombinant wild-type enzyme shows chitinolytic and antifungal activity
gene VCH3, isolation of VCH3 gene promoter, constitutive expression in transgenic tobacco ubiquitously in roots under control of the CMV S35 promoter
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gene VvChi4D, DNA and amino acid sequence determination and analysis
gene win6, expression of WIN6 in tomato plants, Lycopersicon esculentum, via transfection using the potato virus X, PVX, expression levels in transgenic lines, overview
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gene YlCTS1, DNA and amino acid sequence determination, analysis and comparison
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genes chiB and chiA, sequence comparisons and phylogenetic analysis
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isozyme APAP1, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in female Gastrophysa atrocyanea beetles by microinjection in the intersegmental membrane between metathorax and first abdominal segment
isozyme APAP2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in female Gastrophysa atrocyanea beetles by microinjection in the intersegmental membrane between metathorax and first abdominal segment
LysM domain expressed in Escherichia coli
overexpressed in Escherichia coli
-
overexpression in Escherichia coli
overexpression in Escherichia coli in cytosolic and secreted forms
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overexpression in Escherichia coli. Since the protein is produced as insoluble inclusion bodies, the protein is solubilized and refolded
overexpression in Pichia pastoris using a constitutive expression system. The optimal enzyme kinetic parameters for the recombinant protein were identical to those reported for the enzyme isolated from Trichoderma atroviride
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overexpression of a His6-tagged chitinase A 575 amino acid fragment in Escherichia coli strain M15
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overexpression of C-terminally His6-tagged wild-type and mutant enzymes in Pichia pastoris strain GS115 and secretion to the culture medium
overexpression of ChiG in Escherichia coli BL21Star (DE3)
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overexpression of CrChi1 in Escherichia coli strain BL21 (DE3)
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overexpression of wild-type enzyme and mutant enzymes D522N, D522A, D524N, D524A, E526Q and E526A in Escherichia coli
PLChiA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain Origami (DE3) as both soluble and insoluble forms
recombinant expression in Escherichia coli strain BL21 (DE3) as His6-Nus-His6-NaCHIT1 fusion protein. The fusion protein is cleaved by HRV 3C protease resulting in a soluble and active NaCHIT1
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recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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SCChi-1, DNA and amiino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged SCChi-1 in Escherichia coli strain MC1061
single copy gene, species determination and isozyme analysis using the DNA sequence of the gene encoding the polymorphic chitinase
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species determination and isozyme analysis using the DNA sequence of the gene encoding the polymorphic chitinase
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stable expression of SI-CLP in H1299 cells, SI-CLP expression analysis in human cell lines, overview
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subcloning in Escherichia coli strain DH5alpha, expression in Pichia pastoris strain GS115
the chitin-binding domain CBD of the enzyme is fused to genes lpp and ompA and the Lpp-OmpA-ChBD hybrid is expressed in Escherichia coli strain BL21(DE3) directed to the cell surface
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the chitinase gene containing His-tag is expressed in Pichia pastoris
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the recombinant VpChiA and its C-terminal truncated G589 mutantVpChiAG589 encoded in 1881 and 1791 bp DNA fragments, respectively, are expressed in Escherichia coli using the pET-20b(+) expression system
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transgenic tobacco plants expressing an active ChiA protein show reduced damages caused by fungal pathogens and lepidopteran larvae
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