Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.14: chitinase

This is an abbreviated version!
For detailed information about chitinase, go to the full flat file.

Word Map on EC 3.2.1.14

Reaction

chitopentaose
+
H2O
=
chitobiose
 +
chitotriose

Synonyms

1,4-beta-poly-N-acetylglucosaminidase, AcD1ChiA, acid mammalian chitinase, acidic chitinase, acidic mammalian chitinase, active phase-associated protein I, active phase-associated protein II, AD2PF-ChiA, AgChi, alpha-chitinase, AMCase, antifreeze protein, APAP1, APAP2, APAPI, APAPII, AtChiC, B220, bacterial-type chitinase, Bbchit1, BChi14, beta-1,4-poly-N-acetyl glucosamidinase, BjCHI1, BJL200-ChiC1, CF-AG, CF-antigen, CGCHI3, CH5B, ChBDChiC, CHI, Chi-1, CHI-26, Chi-A, Chi-h, Chi1, Chi18A, Chi18B, Chi18C, Chi19, CHI2, Chi2 enzyme, Chi21, Chi22, Chi3, Chi31, Chi35, Chi40, Chi44, Chi46, Chi54, CHI60, Chi67, Chi70, Chi72, Chi78, ChiA, ChiA Nima, ChiA protein, ChiA-HD73, ChiA-Hh59, ChiA-Mt45, ChiA1, ChiA74, ChiB, ChiC, ChiCW, ChiD, ChiE, ChiEN1, ChiF, ChiF1, ChiG, ChiNCTU2, Chit, CHIT 1A, CHIT 1B, CHIT1, Chit1b, Chit33, CHIT42, Chit62, ChitA, ChitIII-1, ChitIII-2, chitinase, chitinase 1, chitinase 18C, chitinase 2, chitinase 60, chitinase 92, chitinase A, chitinase A1, chitinase B, chitinase C, chitinase C1, chitinase Chi255, chitinase F1, chitinase G, chitinase I, chitinase II, chitinase III, chitinase-A, chitinases A, chitinolytic enzyme, chitodextrinase-N-, chitotriosidase, ChiW, CHT1, Cht4, CHT7, ChtII, ChtIII, CiX1, class I chitinase, class II chitinase, class III chitinase, class IV chitinase, class V chitinase, Complement-fixation antigen, CpCHI, CrChi1, CTS1, ECH, ECH1, Ech42, ech46, ELC, endo-chitinase, endochitinase, endochitinase 1, endochitinase-1, endochitinase-2, exochitinase, GAC1, GAC2, GH18 chitinase, group I chitinase, group II chitinase, group III chitinase, hevamine, HoChiA, HoChiB, HoChiC, HschiA1, Is-chiB, LlChi18A, LpChiA, MACase, MF1 antigen, MmChi60, More, MsChi386, MsChi535, NaCHIT1, NaCHIT3, NtChitIV, NtChiV, OfChtI, OsChia1b, OsChia1c, OsChia1cDELTAChBD, OsChia1d, OsChia2a, OsChia2b, OsChia4a, OsChib1a, PF1233, PjChi-1, plant class III chitinase, PLC-A, PLC-B, PLChiA, poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase, poly-beta-glucosaminidase, PrChi-A, Ptchi19p, Ra-ChiC, RCB4, RHBC, sAMC, SCChi-1, SI-CLP, SjChi, SsChi50, stabilin-1 interacting chitinase-like protein, STK_09390, TBC-1, Tfu_0580, Tk-ChiA, TKU028 chitinase, TmChi, TSA1902, UDA, V-CHIA, VpChiA, WIN6, YlCts1p, Ym1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.14 chitinase

Inhibitors

Inhibitors on EC 3.2.1.14 - chitinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1E)-prop-1-en-1-yl 2-(acetylamino)-4-O-[(2R,5R,6R)-5-(acetylamino)-2-(hydroxymethyl)-1,3-oxazinan-6-yl]-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranoside
-
potent inhibition, IC50 is 0.0047 mM, chemo-enzymatic synthesis via allyl penta-N-acetyl-chitopentaose using recombinant NodC from Mesorhizobium loti expressed in Escherichia coli, overview
1,10-phenanthroline
1,2-Cyclohexanedione
-
-
1-butanol
-
about 80% residual activity at 25% (v/v)
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
5 mM, 7% residual activity
2-(piperazin-1-yl)pyridine-3-carboxamide
-
-
2-Acetamido-2-deoxy-D-gluconolactone
-
-
2-Hydroxy-5-nitrobenzyl bromide
-
-
2-mercaptoethanol
2-methyl-3-[[4-(pyridin-2-yl)piperazin-1-yl]methyl]-1H-indole
-
-
2-sufanylethanol
3,4-dinitrophenyl-tetra-N-acetyl-beta-D-chitotetraoside
-
at concentrations above 0.23 mM
4-(4-chlorophenyl)piperazine-1-carboximidamide
-
-
4-chloromercuribenzoate
-
complete inhibition
4-chloromercuribenzoic acid
-
complete inhibition at 2 mM
4-nitrophenyl N,N'-diacetylchitobiose
substrate inhibition at the first catalytic domain with E691
4-O-N-acetyl-beta-D-glucosaminyl-N-acetyl-D-glucosamine
21% inhibition
5,5'-dithio-bis(2-nitrobenzoic acid)
-
2% residual activity at 10 mM
5,5'-dithiobis-(2-nitrobenzoic acid)
-
about 8% residual activity at 2 mM
5-[4-[2-(4-bromophenoxy)ethyl]piperazin-1-yl]-1H-1,2,4-triazol-3-amine
-
-
8-chlorotheophylline
-
acetazolamide
acetonitrile
Ag2+
-
1 mM, complete inhibition of hydrolysis of 4-methylumbelliferyl-N,N'-diacetylchitobiose and 4-methylumbelliferyl-N,N',N''-triacetylchitotriose
AlCl3
-
deletorious effect at 0.01 M
allosamidin
antiserum
-
anti-Chi-A and anti-Chi-B
-
argadin
thermodynamics and enzyme-bound crystal structure, detailed overview
argifin
thermodynamics and enzyme-bound crystal structure, detailed overview
AsO2-
benzo[a]pyrene
-
noncompetitive
benzo[e]pyrene
-
noncompetitive
benzo[k] fluoranthene
-
noncompetitive
CaCl2
-
1 mM, 27% inhibition of activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, no inhibition of activity with 4-methylumbelliferyl-(GlcNAc)2
Caffeine
two caffeine molecules bind to CrChi1 in subsites -1 to +1 in the substrate-binding domain, binding structure, overview
chitin
-
colloidal and alpha-chitin
chitobiose
chitopentaose
-
-
chitotriose
closantel
-
competitive inhibition
colloidal chitin
-
substrate inhibition
-
Cr2+
56% residual activity at 1 mM
Cr6+
-
80.9% residual activity at 1 mM
CuSO4
D-galactosamine
-
-
D-glucose
18% inhibition
diacetylchitobiose
dimethyl sulfoxide
-
-
dinitrosalicylic acid
dithiothreitol
erythritol
-
relative inhibition of 18.1%, three concentrations of 10, 50, and 100 mM tested
ethyl acetate
glutathione
guanidine hydrochloride
-
-
HgCl2
iodoacetamide
iodoacetic acid
isooctane
kinetin
competitive
L-cysteine
LKB ampholytes
-
-
-
methylallosamidin
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside
-
-
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside
-
-
Mo2+
-
50% inhibition at a concentration of 1 mM
monoiodoacetate
-
-
N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide
-
-
N-acetyl-D-glucosamine
24% inhibition
N-acetylgalactosamine
-
76.2% relative inhibition, three concentrations of 10, 50, and 100 mM tested
N-acetylglucosamine
N-Acetylimidazole
-
94% residual activity at 2 mM
N-acylimidazole
-
-
N-bromosuccinimide
N-Chlorosuccinimide
-
both isoenzymes
N-ethyl-2-(4-methylpiperazin-1-yl)pyridine-3-carboxamide
-
-
N-ethylmaleimide
n-heptane
-
about 80% residual activity at 25%(v/v)
n-hexadecane
-
about 70% residual activity at 25%(v/v)
Na+
strong inhibition
p-chloromercuribenzoate
p-chloromercuribenzoic acid
-
complete inhibition at 2 mM
p-hydroxymercuribenzoate
p-nitrophenyl-GlcNAc
-
10 mM, 50% inhibition
Pd2+
-
complete inhibition at 2.5 mM
pentaacetylchitopentaose
-
competitive inhibitor
perylene
-
noncompetitive
phenylmethanesulfonylflouride
phenylmethylsulfonyl fluoride
-
64% residual activity at 5 mM
phospholipase
-
phospholipases A,C, and D
-
polyethyleneglycol 400
-
-
-
tetraacetylchitotetraose
thimerosal
Toluene
-
about 80% residual activity at 25%(v/v)
triacetylchitotriose
-
competitive inhibitor
Triton X-100
Trypsin
-
small amounts, pH more than 7.4
-
Tween 20
-
40% inhibition at 2%
Tween 40
-
40% inhibition at 2%
Tween-20
-
21% inhibition at 2%
Tween-80
-
10%, 49% residual activity
ZnSO4
additional information
-