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3.2.1.14: chitinase

This is an abbreviated version!
For detailed information about chitinase, go to the full flat file.

Word Map on EC 3.2.1.14

Reaction

chitopentaose
+
H2O
=
chitobiose
 +
chitotriose

Synonyms

1,4-beta-poly-N-acetylglucosaminidase, AcD1ChiA, acid mammalian chitinase, acidic chitinase, acidic mammalian chitinase, active phase-associated protein I, active phase-associated protein II, AD2PF-ChiA, AgChi, alpha-chitinase, AMCase, antifreeze protein, APAP1, APAP2, APAPI, APAPII, AtChiC, B220, bacterial-type chitinase, Bbchit1, BChi14, beta-1,4-poly-N-acetyl glucosamidinase, BjCHI1, BJL200-ChiC1, CF-AG, CF-antigen, CGCHI3, CH5B, ChBDChiC, CHI, Chi-1, CHI-26, Chi-A, Chi-h, Chi1, Chi18A, Chi18B, Chi18C, Chi19, CHI2, Chi2 enzyme, Chi21, Chi22, Chi3, Chi31, Chi35, Chi40, Chi44, Chi46, Chi54, CHI60, Chi67, Chi70, Chi72, Chi78, ChiA, ChiA Nima, ChiA protein, ChiA-HD73, ChiA-Hh59, ChiA-Mt45, ChiA1, ChiA74, ChiB, ChiC, ChiCW, ChiD, ChiE, ChiEN1, ChiF, ChiF1, ChiG, ChiNCTU2, Chit, CHIT 1A, CHIT 1B, CHIT1, Chit1b, Chit33, CHIT42, Chit62, ChitA, ChitIII-1, ChitIII-2, chitinase, chitinase 1, chitinase 18C, chitinase 2, chitinase 60, chitinase 92, chitinase A, chitinase A1, chitinase B, chitinase C, chitinase C1, chitinase Chi255, chitinase F1, chitinase G, chitinase I, chitinase II, chitinase III, chitinase-A, chitinases A, chitinolytic enzyme, chitodextrinase-N-, chitotriosidase, ChiW, CHT1, Cht4, CHT7, ChtII, ChtIII, CiX1, class I chitinase, class II chitinase, class III chitinase, class IV chitinase, class V chitinase, Complement-fixation antigen, CpCHI, CrChi1, CTS1, ECH, ECH1, Ech42, ech46, ELC, endo-chitinase, endochitinase, endochitinase 1, endochitinase-1, endochitinase-2, exochitinase, GAC1, GAC2, GH18 chitinase, group I chitinase, group II chitinase, group III chitinase, hevamine, HoChiA, HoChiB, HoChiC, HschiA1, Is-chiB, LlChi18A, LpChiA, MACase, MF1 antigen, MmChi60, More, MsChi386, MsChi535, NaCHIT1, NaCHIT3, NtChitIV, NtChiV, OfChtI, OsChia1b, OsChia1c, OsChia1cDELTAChBD, OsChia1d, OsChia2a, OsChia2b, OsChia4a, OsChib1a, PF1233, PjChi-1, plant class III chitinase, PLC-A, PLC-B, PLChiA, poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase, poly-beta-glucosaminidase, PrChi-A, Ptchi19p, Ra-ChiC, RCB4, RHBC, sAMC, SCChi-1, SI-CLP, SjChi, SsChi50, stabilin-1 interacting chitinase-like protein, STK_09390, TBC-1, Tfu_0580, Tk-ChiA, TKU028 chitinase, TmChi, TSA1902, UDA, V-CHIA, VpChiA, WIN6, YlCts1p, Ym1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.14 chitinase

pH Stability

pH Stability on EC 3.2.1.14 - chitinase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11
-
52% activity left after 1 h
655820
2 - 6
-
the half-life times of the enzyme at pH 2.0, 3.0, 4.0, 5.0, and 6.0 are 18, 14, 10, 6, and 2 h, respectively
750244
2.5 - 8
-
-
393513
3 - 10
3 - 11
3 - 12
purified native deglycosylated enzyme, 32°C, completely stable
729440
3 - 5
-
the half-life times of the enzyme at pH 3.0, 4.0, and 5.0 are 10, 8, and 6 h, respectively
750770
3 - 6.5
-
-
393508
3 - 7
3 - 9
3.4
purified recombinant enzyme, stable
692328
4 - 10
4 - 11
-
707769
4 - 6
-
Chi-A
393545
4 - 8
4 - 9
4 - 9.5
4.2 - 10
purified recombinant enzyme, stable
718222
4.5 - 6.5
4.5 - 7
4.5 - 9
-
742728
5 - 10
5 - 11
5 - 7
5 - 8
5 - 9
5 - 9.5
-
highest stability at pH 8.5, over 80% activity at pH 6.0-9.2, 70% activity remaining at pH 9.5, 25% at pH 5.0, profile overview
729009
5.5
-
maximum stabtility at pH 5.5
393513
5.5 - 8.5
6 - 11
-
purified enzyme, stable between pH 6.0 and pH 11.0, but unstable below pH 5.0 and above pH 12
717644
6 - 12
-
isoenzyme 2
393539
6 - 7
-
stable
717981
6 - 8
6 - 9
6.5 - 8.5
-
the enzyme maintains over 90% of its activity after 40 min
726791
7
-
30°C, 10 min, stable up to, chitinase 1
681710
7 - 10
purified recombinant enzyme, 1 h, over 80% activity remaining, most stable at pH 8.5
717132
8
-
purified enzyme, 60°C, 48 h, loss of 20% activity
729427
8 - 10
purified recombinant enzyme, 10 days, no loss of activity at 4°C
692256
9
-
purified enzyme, 60°C, 48 h, loss of 30% activity
729427
9 - 11
-
80% activity remains
393540
additional information
-
the purified enzyme is very stable at alkaline pH
729009