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3.2.1.14: chitinase

This is an abbreviated version!
For detailed information about chitinase, go to the full flat file.

Word Map on EC 3.2.1.14

Reaction

chitopentaose
+
H2O
=
chitobiose
 +
chitotriose

Synonyms

1,4-beta-poly-N-acetylglucosaminidase, AcD1ChiA, acid mammalian chitinase, acidic chitinase, acidic mammalian chitinase, active phase-associated protein I, active phase-associated protein II, AD2PF-ChiA, AgChi, alpha-chitinase, AMCase, antifreeze protein, APAP1, APAP2, APAPI, APAPII, AtChiC, B220, bacterial-type chitinase, Bbchit1, BChi14, beta-1,4-poly-N-acetyl glucosamidinase, BjCHI1, BJL200-ChiC1, CF-AG, CF-antigen, CGCHI3, CH5B, ChBDChiC, CHI, Chi-1, CHI-26, Chi-A, Chi-h, Chi1, Chi18A, Chi18B, Chi18C, Chi19, CHI2, Chi2 enzyme, Chi21, Chi22, Chi3, Chi31, Chi35, Chi40, Chi44, Chi46, Chi54, CHI60, Chi67, Chi70, Chi72, Chi78, ChiA, ChiA Nima, ChiA protein, ChiA-HD73, ChiA-Hh59, ChiA-Mt45, ChiA1, ChiA74, ChiB, ChiC, ChiCW, ChiD, ChiE, ChiEN1, ChiF, ChiF1, ChiG, ChiNCTU2, Chit, CHIT 1A, CHIT 1B, CHIT1, Chit1b, Chit33, CHIT42, Chit62, ChitA, ChitIII-1, ChitIII-2, chitinase, chitinase 1, chitinase 18C, chitinase 2, chitinase 60, chitinase 92, chitinase A, chitinase A1, chitinase B, chitinase C, chitinase C1, chitinase Chi255, chitinase F1, chitinase G, chitinase I, chitinase II, chitinase III, chitinase-A, chitinases A, chitinolytic enzyme, chitodextrinase-N-, chitotriosidase, ChiW, CHT1, Cht4, CHT7, ChtII, ChtIII, CiX1, class I chitinase, class II chitinase, class III chitinase, class IV chitinase, class V chitinase, Complement-fixation antigen, CpCHI, CrChi1, CTS1, ECH, ECH1, Ech42, ech46, ELC, endo-chitinase, endochitinase, endochitinase 1, endochitinase-1, endochitinase-2, exochitinase, GAC1, GAC2, GH18 chitinase, group I chitinase, group II chitinase, group III chitinase, hevamine, HoChiA, HoChiB, HoChiC, HschiA1, Is-chiB, LlChi18A, LpChiA, MACase, MF1 antigen, MmChi60, More, MsChi386, MsChi535, NaCHIT1, NaCHIT3, NtChitIV, NtChiV, OfChtI, OsChia1b, OsChia1c, OsChia1cDELTAChBD, OsChia1d, OsChia2a, OsChia2b, OsChia4a, OsChib1a, PF1233, PjChi-1, plant class III chitinase, PLC-A, PLC-B, PLChiA, poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase, poly-beta-glucosaminidase, PrChi-A, Ptchi19p, Ra-ChiC, RCB4, RHBC, sAMC, SCChi-1, SI-CLP, SjChi, SsChi50, stabilin-1 interacting chitinase-like protein, STK_09390, TBC-1, Tfu_0580, Tk-ChiA, TKU028 chitinase, TmChi, TSA1902, UDA, V-CHIA, VpChiA, WIN6, YlCts1p, Ym1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.14 chitinase

Specific Activity

Specific Activity on EC 3.2.1.14 - chitinase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00032
-
soluble protein fraction, N,N',N'',N'''-tetraacetylchitotetraose as substrate
0.00037
-
soluble protein fraction, N,N'-diacetylchitobiose as substrate
0.00078
-
insoluble protein fraction, N,N',N'',N'''-tetraacetylchitotetraose as substrate
0.00135
-
insoluble protein fraction, N,N'-diacetylchitobiose as substrate
0.0025
-
isoenzyme 3
0.0047 - 0.01
-
isoenzymes C2 and B, other isoenzymes in between
0.0069
-
purified CHT1, substrate chitin
0.0097
-
isoenzyme 6
0.0123
purified recombinant ChiC
0.0127
-
isoenzyme 7
0.015
0.0193
-
isoenzyme 4
0.02
mutant W82A, substrate glycol chitin
0.0229
-
purified CHT1, substrate colloidal chitin
0.0345
-
after chitin affinity purification step
0.0391
-
isoenzyme 2
0.0421
-
isoenzyme 5
0.057
-
purified recombinant chitinase
0.075
pH and temperature not specified in the publication, substrate: colloidal chitin
0.12
-
carboxymethyl-chitin
0.175
-
purified enzyme, pH 7.0, 37°C
0.19
-
squid chitin
0.2291
-
isoenzyme 1
0.24
-
glycol chitin
0.25
-
p-nitrophenol-beta-D-lactopyranoside or p-nitrophenol-beta-D-galactopyranoside as substrate
0.294
substrate 4-nitrophenyl-chitobiose, pH 6.0, 37°C
0.35
-
p-nitrophenol-beta-D-cellobioside as substrate
0.38
mutant W72A/W82A, substrate glycol chitin
0.472
-
isoenzyme II
0.5
-
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose
0.513
-
pH 7.3, 40°C
0.547
-
isoenzyme I
0.62
-
purified enzyme
0.63
-
p-nitrophenol-beta-D-mannopyranoside as substrate
0.641
-
isoenzyme III
0.68
-
chitosan, 90% deacetylated chitin
0.71
purified recombinant enzyme, pH 5.5, 37°C, substrate 4-nitrophenyl-N,N'-diacetyl-D-chitobiose
0.85
-
with ethylene glycol chitin as substrate
0.91
purified recombinant enzyme, substrate 4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose
0.96
-
p-nitrophenol-beta-D-maltoside as substrate
1.02
-
after Sephacryl S-200 purification step with colloidal chitin as substrate
1.16
-
p-nitrophenyl beta-D-N,N'-diacetylchitobiose
1.26
-
p-nitrophenol-beta-D-glucopyranoside as substrate
1.7
-
after sephacryl S-100 HR purification step
1.84
-
regenerated chitin
10.48
-
chitotetraose
104.6
-
39 kDa form
1120
-
isoenzyme PLC-B
116
-
OsChia2b with glycol chitin as substrate
117
-
purified recombinant nonglycosylated Chi3
12.3
-
purified extracellular chitinase
12.6
-
isoenzyme II
126.7
-
swollen chitin
13.6
enzyme from non-inducing medium
135.1
-
GBC-b, glycol chitin
137
-
after Sephadex G-100 purification step
139
wild-type enzyme, substrate GlcNAc hexasaccharide
140.6
-
purified enzyme, pH 8.0, 35°C, substrate colloidal chitin
150
-
activity of secreted enzyme after Q-high performance column chromatography purification step
151
-
activity of cytosolic enzyme after Q-high performance column chromatography purification step
1546
-
isoenzyme PLC-A
159.7
-
GBC-a, glycol chitin
16.4
-
purified recombinant enzyme, pH 7.5, 43°C, substrate crystalline chitin
16600
substrate chitotriose, pH 6.0, 40°C, wild-type enzyme
17.4
-
chitinase 1
17.7
-
chitinase 2
18.1
substrate glycol chitin, pH 4.5, 50°C
18.6
-
isoenzyme I
191
-
wild-type enzyme, substrate: colloidal chitin
197.1
-
purified native enzyme, pH 4.0, 50°C, substrate colloidal chitin
2 - 2.5
-
purified enzyme
2.07
-
p-nitrophenol-beta-D-N,N'-diacetylchitobioside as substrate as substrate
2.1
-
OsChia1c with colloidal chitin as substrate
2.3
-
OsChia2b with colloidal chitin as substrate
2.397
-
purified native enzyme, pH 7.0, 37°C, affinity purification method
2.53
mutant W72A, substrate glycol chitin
2.7
substrate tri-N-acetyl-chitotriose, pH 4.5, 50°C
2.95
purified enzyme
20.3
-
purified enzyme, pH 6.8, 45°C
2211
-
purified OsChia1b, substrate WS-chitin
225
-
purified OsChia1b, substrate colloidal chitin
23.4
mutant W82A, substrate GlcNAc hexasaccharide
230
-
chitosan as substrate
232
-
wild-type enzyme, substrate: glycol chitin
2360
-
purified enzyme, pH 7.0, 40°C, substrate colloidal chitin
2382
-
purified recombinant enzyme, pH 7.5, 43°C, substrate 4-nitrophenyl N-acetyl-beta-D-glucosamine
24.5
-
colloidal chitin
25
purified recombinant enzyme
25.85
pH 5.5, 42°C
251
mutant W72A, substrate GlcNAc hexasaccharide
253
-
purified OsChia1b, substrate glycol chitin
254
-
purified OsChia1b, substrate GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc
255
-
glycol chitin
26.2
substrate colloidal chitin, pH 8.0, 50°C
265
-
DELTAloop II enzyme, substrate: colloidal chitin
270
-
cellulose as substrate
289
-
purified recombinant glycosylated Chi3
3.04
-
isoenzyme HoChiA
3.1
substrate chitosan, pH 8.0, 50°C
3.2
-
swollen chitin
3.33
-
purified enzyme, substrate 4-nitrophenyl N-acetylchitobioside
3.6
-
p-nitrophenyl N-acetyl-beat-D-glucosaminide
3.7
-
after Sephadex G200 purification step
310
-
chitin as substrate
312
-
after Sephadex G-75 purification step
31600
substrate chitotetraose, pH 6.0, 40°C, wild-type enzyme
3458
-
purified enzyme, pH 5.0, 37°C
35.5
-
purified enzyme, pH 7.0, 37°C
363.2
substrate: N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose
399
-
mutant enzyme W159A, substrate: colloidal chitin
4
substrate powdery chitin, pH 8.0, 50°C
4.6
-
after Sephadex G-100 purification step
4.78
-
chitopentaose, Chi-B
426.3
-
after affinity chromatography purification step
440000
-
after Sephacryl S-100 HR P-II-1 purification step
479
-
DELTAloop II enzyme, substrate: glycol chitin
49.6
-
purified native enzyme
492.4
-
purified enzyme, pH 5.0, 50°C
5.14
-
chitopentaose, Chi-A
5.7
-
20°C, pH 6.0
52
-
OsChia1c with colloidal glycol as substrate
52600
substrates chitopentaose and chitohexaose, pH 6.0, 40°C, wild-type enzyme
546
-
after gel slicing purification step
5800
-
soluble hyphae extract fraction
59.9
-
purified recombinant enzyme, pH 7.5, 43°C, substrate colloidal chitin
6.1
Thermochaetoides thermophila
substrate colloidal chitin, pH 8.0, 50°C
6.23
purified recombinant enzyme, pH 8.0, 32°C, substrate glycolchitin
6.31
wild-type enzyme, substrate glycol chitin
6.463
-
purified native enzyme, pH 7.0, 37°C, anion exchange purification method
6.51
-
purified enzyme, pH 5.0, 37°C, substrate colloidal chitin
6.74
-
chitobiose
62.4
-
purified enzyme, pH 6.5, 60°C
6688
-
after CM-Sepharose column purification step
68
-
pH 5.4, 50°C
688000
-
GAC1 after phenyl-sepharose purification step
7.4
enzyme from inducing medium
731
-
after Sephadex G-75 purification step
756300
-
GAC2 after phenyl-sepharose purification step
8.06
-
chitosan, 80% deacetylated chitin
8.57
-
chitotriose
83
-
OsChia1cDELTACBD with glycol chitin as substrate
9.73
-
chitosan, 70% deacetylated chitin
94.21
-
purified enzyme, pH 7.0, 37°C
946
-
mutant enzyme W159A, substrate: glycol chitin
additional information