3.2.1.14: chitinase
This is an abbreviated version!
For detailed information about chitinase, go to the full flat file.
Word Map on EC 3.2.1.14
-
3.2.1.14
-
biocontrol
-
colloidal
-
trichoderma
-
chitin-binding
-
fusarium
-
pathogenesis-related
-
serratia
-
n-acetylglucosamine
-
marcescens
-
chitosan
-
3-like
-
chi3l1
-
solani
-
freezing
-
gaucher
-
glcnac
-
beta-1,3-glucanase
-
phytopathogenic
-
cellulase
-
rhizoctonia
-
oxysporum
-
cinerea
-
botrytis
-
glucanase
-
harzianum
-
mycelial
-
cuticle
-
defense-related
-
hysteresis
-
rhizosphere
-
colletotrichum
-
chitooligosaccharides
-
molt
-
n-acetyl-d-glucosamine
-
alternaria
-
flounder
-
circulans
-
chitosanase
-
entomopathogenic
-
recrystallization
-
sclerotiorum
-
transglycosylation
-
viride
-
beauveria
-
bassiana
-
pythium
-
nucleopolyhedrovirus
-
anthracnose
-
glucocerebrosidase
-
gloeosporioides
-
environmental protection
-
molecular biology
-
analysis
-
synthesis
-
pharmacology
-
industry
-
degradation
-
medicine
-
biotechnology
-
agriculture
- 3.2.1.14
-
biocontrol
-
colloidal
- trichoderma
-
chitin-binding
- fusarium
-
pathogenesis-related
- serratia
- n-acetylglucosamine
- marcescens
- chitosan
-
3-like
-
chi3l1
- solani
-
freezing
- gaucher
- glcnac
- beta-1,3-glucanase
-
phytopathogenic
- cellulase
- rhizoctonia
- oxysporum
- cinerea
- botrytis
- glucanase
- harzianum
- mycelial
- cuticle
-
defense-related
-
hysteresis
-
rhizosphere
- colletotrichum
- chitooligosaccharides
-
molt
- n-acetyl-d-glucosamine
- alternaria
- flounder
- circulans
- chitosanase
-
entomopathogenic
-
recrystallization
- sclerotiorum
-
transglycosylation
- viride
- beauveria
- bassiana
- pythium
- nucleopolyhedrovirus
-
anthracnose
- glucocerebrosidase
- gloeosporioides
- environmental protection
- molecular biology
- analysis
- synthesis
- pharmacology
- industry
- degradation
- medicine
- biotechnology
- agriculture
Reaction
Synonyms
1,4-beta-poly-N-acetylglucosaminidase, AcD1ChiA, acid mammalian chitinase, acidic chitinase, acidic mammalian chitinase, active phase-associated protein I, active phase-associated protein II, AD2PF-ChiA, AgChi, alpha-chitinase, AMCase, antifreeze protein, APAP1, APAP2, APAPI, APAPII, AtChiC, B220, bacterial-type chitinase, Bbchit1, BChi14, beta-1,4-poly-N-acetyl glucosamidinase, BjCHI1, BJL200-ChiC1, CF-AG, CF-antigen, CGCHI3, CH5B, ChBDChiC, CHI, Chi-1, CHI-26, Chi-A, Chi-h, Chi1, Chi18A, Chi18B, Chi18C, Chi19, CHI2, Chi2 enzyme, Chi21, Chi22, Chi3, Chi31, Chi35, Chi40, Chi44, Chi46, Chi54, CHI60, Chi67, Chi70, Chi72, Chi78, ChiA, ChiA Nima, ChiA protein, ChiA-HD73, ChiA-Hh59, ChiA-Mt45, ChiA1, ChiA74, ChiB, ChiC, ChiCW, ChiD, ChiE, ChiEN1, ChiF, ChiF1, ChiG, ChiNCTU2, Chit, CHIT 1A, CHIT 1B, CHIT1, Chit1b, Chit33, CHIT42, Chit62, ChitA, ChitIII-1, ChitIII-2, chitinase, chitinase 1, chitinase 18C, chitinase 2, chitinase 60, chitinase 92, chitinase A, chitinase A1, chitinase B, chitinase C, chitinase C1, chitinase Chi255, chitinase F1, chitinase G, chitinase I, chitinase II, chitinase III, chitinase-A, chitinases A, chitinolytic enzyme, chitodextrinase-N-, chitotriosidase, ChiW, CHT1, Cht4, CHT7, ChtII, ChtIII, CiX1, class I chitinase, class II chitinase, class III chitinase, class IV chitinase, class V chitinase, Complement-fixation antigen, CpCHI, CrChi1, CTS1, ECH, ECH1, Ech42, ech46, ELC, endo-chitinase, endochitinase, endochitinase 1, endochitinase-1, endochitinase-2, exochitinase, GAC1, GAC2, GH18 chitinase, group I chitinase, group II chitinase, group III chitinase, hevamine, HoChiA, HoChiB, HoChiC, HschiA1, Is-chiB, LlChi18A, LpChiA, MACase, MF1 antigen, MmChi60, More, MsChi386, MsChi535, NaCHIT1, NaCHIT3, NtChitIV, NtChiV, OfChtI, OsChia1b, OsChia1c, OsChia1cDELTAChBD, OsChia1d, OsChia2a, OsChia2b, OsChia4a, OsChib1a, PF1233, PjChi-1, plant class III chitinase, PLC-A, PLC-B, PLChiA, poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase, poly-beta-glucosaminidase, PrChi-A, Ptchi19p, Ra-ChiC, RCB4, RHBC, sAMC, SCChi-1, SI-CLP, SjChi, SsChi50, stabilin-1 interacting chitinase-like protein, STK_09390, TBC-1, Tfu_0580, Tk-ChiA, TKU028 chitinase, TmChi, TSA1902, UDA, V-CHIA, VpChiA, WIN6, YlCts1p, Ym1
ECTree
3.2.1.14 chitinaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 3.2.1.14 - chitinase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1 - 50
100
20 - 50
20 - 70
25 - 65
-
highest stability at 40°C, over 80% activity at 25-50°C, 50% activity remaining at 60°C, 30% at 65°C, profile overview
30
30 - 80
-
the enzyme has no activity when incubated at 80°C for 2 h. However, it retains more than 68 % of its initial activity after incubation at 30, 40, and 50°C for 2 h. This enzyme also retains more than 47 % of its initial activity after incubation at 30, 40, and 50°C for 24 h
37
40
40 - 60
40 - 70
the enzyme remains stable (more than 90% activity) after 10 min at 40-70°C. After 20 min at 40-50°C, the enzyme retains more than 90% activity, while at 60°C the enzyme activity drops to 50%. The enzyme is inactive after 30 min at 70°C
45
50
55
55 - 65
60
65
70
70 - 100
-
the half-life times at 70, 80, 90, and 100°C in the absence of Mg2+ are 8, 5, 3, and 1 h, respectively. In the presence of 2 mM Mg2+, the half-life times of the enzyme increase to 10, 7, 4, and 2 h, respectively
75
80
85
90
additional information
100
-
purified enzyme, pH 5.0, with 2 mM Co2+: half-life is 4 h, inactivation after 6 h, without Co2+: half-life is 2 h, inactivation after 8 h
20 - 50
-
pH 7.0, 10 min, purified mutant enzyme, stable at, over 90% activity within this range
20 - 70
-
enzyme activity decreases with increasing incubation temperature after being incubated for 30 min at 20-70°C
20 - 70
-
pH 7.0, 10 min, purified wild-type enzyme, stable at, over 90% activity within this range
30
-
ChiC1: 40% of activity remained, ChiA and ChiB: 10% of activity remained
40
purified native deglycosylated enzyme, pH 8.0, 15 min, completely stable
40 - 60
purified recombinant His-tagged enzyme, 1 h, 80% remaining activity within this range
40 - 60
the enzyme activity is retained for 60 min at temperatures up to 40°C. Isoform Chi35 has a melting temperature of 60.8°C
40 - 60
the enzyme activity is retained for 60 min at temperatures up to 40°C. Isoform Chi78 is almost completely inactivated in 60 min at 60°C and shows a melting temperature of 51.5°C
45
-
the enzyme demonstrates thermal stability with 80% activity remaining at 45°C within 2 h
50
-
the purified chitinase retains 68% of its initial activity after 2 h at 50°C
50
-
30 min, 25% loss of activity of VpChiA and 5% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589
55 - 65
the enzyme is stable up to 55°C with a thermal denaturing half-life of 43, 102 and 658 min at 65°C, 60°C, and 55°C, respectively
55 - 65
the enzyme os stable at temperatures below 55°C, since more than 85% of its original activities are retained after incubation at different temperatures for 30 min. The thermal denaturation half-lives of the enzyme at 55, 60 and 65°C are 658, 102 and 43 min, respectively
55 - 65
the half-lives of the enzyme at 55, 60, and 65°C are 323, 97, and 29 min, respectively
60
-
purified enzyme, 1.31fold activation of enzyme activity after 60 min, remaining activity after 100 min
60
purified native deglycosylated enzyme, pH 8.0, 15 min, over 90% activity remaining
60
ChiA exhibits an endothermic irreversible transition at an apparent Tm of 60.7°C
70
purified native deglycosylated enzyme, pH 8.0, 15 min, over 60% activity remaining
70
-
1 h, 25 mM acetate buffer, pH 5.2, half-life is 3 days, 80% activity remaining after 100 h
70
-
purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 10 h, half-life is 18 h, about 10% activity remaining after 24 h, without Co2+: completely stable for 6 h, half-life is 14 h, inactivation after 20 h
75
purified native deglycosylated enzyme, pH 8.0, 15 min, 10% activity remaining
80
-
purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 4 h, half-life is 12 h, inactivation after 16 h, without Co2+: completely stable for 2 h, half-life is 10 h, inactivation after 18 h
80
-
ChiADELTA4: over 60 min in a pH range from 5-9, ChiADELTA5: over 60 min in a pH range from 4-9
80
-
30 min, 55% loss of activity of VpChiA and 20% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589
90
-
purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 2 h, half-life is 8 h, inactivation after 12 h, without Co2+: half-life is 6 h, inactivation after 14 h
90
-
30 min, 70% loss of activity of VpChiA and 60% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589
additional information
the enzyme stability at 18°C and 28°C is similar to the stability at 4°C
additional information
-
the enzyme stability at 18°C and 28°C is similar to the stability at 4°C
additional information
the PrChi-A LysM domains are very thermostable, which is probably conferred in large part by disulfides
additional information
-
the PrChi-A LysM domains are very thermostable, which is probably conferred in large part by disulfides
