3.1.8.1: aryldialkylphosphatase
This is an abbreviated version!
For detailed information about aryldialkylphosphatase, go to the full flat file.
Word Map on EC 3.1.8.1
-
3.1.8.1
-
arylesterase
-
paraoxonase-1
-
cholesterol
-
nerve
-
atherosclerosis
-
cardiovascular
-
low-density
-
insecticide
-
triglyceride
-
coronary
-
apolipoproteins
-
acetylcholinesterase
-
malondialdehyde
-
diminuta
-
poison
-
soman
-
p-nitrophenol
-
warfare
-
sarin
-
bioremediation
-
chlorpyrifos
-
vx
-
antiatherogenic
-
decontamination
-
bioscavenger
-
atherogenesis
-
cholinesterase
-
phenylacetate
-
medicine
-
binuclear
-
phosphotriesters
-
atherogenic
-
flavobacterium
-
carboxylesterase
-
hdl-cholesterol
-
apoa-i
-
butyrylcholinesterase
-
fluorophosphate
-
surface-expressed
-
diazoxon
-
tabun
-
thiolactone
-
g-type
-
salt-stimulated
-
triesters
-
malathion
-
ochrobactrum
-
sphingobium
-
radiobacter
-
cyclosarin
-
loligo
-
synthesis
-
environmental protection
-
drug development
-
degradation
-
biotechnology
-
diagnostics
-
agriculture
-
analysis
-
nutrition
- 3.1.8.1
- arylesterase
- paraoxonase-1
- cholesterol
- nerve
- atherosclerosis
- cardiovascular
-
low-density
-
insecticide
- triglyceride
- coronary
-
apolipoproteins
- acetylcholinesterase
- malondialdehyde
- diminuta
-
poison
- soman
- p-nitrophenol
-
warfare
- sarin
-
bioremediation
- chlorpyrifos
- vx
-
antiatherogenic
-
decontamination
-
bioscavenger
- atherogenesis
- cholinesterase
- phenylacetate
- medicine
-
binuclear
-
phosphotriesters
-
atherogenic
- flavobacterium
- carboxylesterase
-
hdl-cholesterol
- apoa-i
- butyrylcholinesterase
- fluorophosphate
-
surface-expressed
- diazoxon
- tabun
-
thiolactone
-
g-type
-
salt-stimulated
-
triesters
- malathion
- ochrobactrum
- sphingobium
- radiobacter
- cyclosarin
-
loligo
- synthesis
- environmental protection
- drug development
- degradation
- biotechnology
- diagnostics
- agriculture
- analysis
- nutrition
Reaction
Synonyms
A-esterase, A7Q26_23485, aminopeptidase P, AMPP, aryldialkylphosphatase, arylesterase, aryltriphosphatase, bacterial phosphotriesterase, DFPase, diisopropyl fluorophosphatase, esterase B1, esterase E4, esterase, organophosphate, esterase, paraoxon, esterase, pirimiphos-methyloxon, G3C9 rePON1, h-PON1, HAD, haloalkylphosphorus hydrolase, HDL-associated esterase/lactonase paraoxonase 1, HDL-PON1, high activity paraoxonase, high-density lipoprotein-associated esterase/lactonase, human paraoxonase 1, HuPON1, inner membrane protein YiaH, lactonase SsoPox, lactonase/phosphotriesterase, low activity paraoxonase, methyl parathion hydrolase, More, Mph, mPHP, OP hydrolase, OP-hydrolase, OP-hydrolyzing enzyme, OPA anhydrase, opd, OpdA, OpdD, OPH, OPHC2, organophosphate hydrolase, organophosphorous hydrolase, organophosphorus acid anhydrase, organophosphorus hydrolase, organophosphorus pesticide hydrolase, organophosphorus-hydrolyzing enzyme, paraoxon hydrolase, paraoxonase, paraoxonase 1, paraoxonase 1A, paraoxonase 3, paraoxonase-1, paraoxonase-2, paraoxonase1, parathion hydrolase, phosphotriesterase, phosphotriesterase homology protein, phosphotriesterase-like lactonase, PHP, pirimiphos-methyloxon esterase, PLL, PO.ase, PON, PON 1, PON-1, PON-aryl, PON-para, PON1, PON1A, PON2, PON3, POX, PTE, PTE S5, Rv0230c, SACI2140, Saci_2140, SacPox, Sb-PTE, serum paraoxonase, serum paraoxonase 1, SisLac, SisPox, Sso, SSO2522, SsoPox, type A paraoxonase, type B paraoxonase, VmoLac, Vmut2255, VmutPLL
ECTree
3.1.8.1 aryldialkylphosphataseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.8.1 - aryldialkylphosphatase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1E)-but-1-en-1-yl dibutyl phosphate + H2O
dibutyl phosphate + ?
-
-
-
?
(RS)-propan-2-yl methylphosphonofluoridate + H2O
isopropyl phosphate methylphosphonate + fluoride
i.e. sarin
-
-
?
(S)-[2-(diethylamino)ethyl] O,O-diethyl phosphorothioate + H2O
diethyl phosphate + 2-(diethylamino)ethane-1-thiol
-
-
-
?
(S)-[2-(diethylamino)ethyl] O,O-dimethyl phosphorothioate + H2O
dimethyl phosphate + 2-(diethylamino)ethane-1-thiol
-
-
-
?
(S)-[2-(diethylamino)ethyl] O-(2-methylpropyl) methylphosphonothioate + H2O
2-methylpropyl methylphosphonate + 2-(diethylamino)ethane-1-thiol
-
-
-
?
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate + H2O
diethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol
-
-
-
?
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-dimethyl phosphorothioate + H2O
dimethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol
-
-
-
?
(S)-[2-[di(propan-2-yl)amino]ethyl] O-ethyl methylphosphonothioate + H2O
ethyl methylphosphonate + [di(propan-2-yl)amino]ethane-1-thiol
-
-
-
?
1,2,2-trimethylpropyl methylphosphonofluoridate + H2O
3,3-dimethylbutan-2-yl methylphosphonate + HF
-
-
-
-
?
1-methylethyl 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl methylphosphonate + H2O
?
-
-
-
?
1-methylethyl 4-methyl-2-oxo-2H-chromen-7-yl methylphosphonate + H2O
?
-
-
-
?
1-methylethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O
4-nitrophenol + 1-methylethyl hydrogen (1-methylpropyl)phosphonate
1-methylethyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + 1-methylethyl hydrogen methylphosphonate
1-methylpropyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + 1-methylpropyl hydrogen methylphosphonate
1-palmitoyl-2-(5-oxo)valeroyl-sn-glycero-3-phosphocholine + H2O
lysophosphatidylcholine + ?
-
-
-
?
2,4-dinitrophenyl diethyl phosphate + H2O
2,4-dinitrophenol + diethyl phosphate
-
-
-
?
2,6-difluorophenyl diethyl phosphate + H2O
2,6-difluorophenol + diethyl phosphate
-
-
-
?
2-fluoro-4-nitrophenyl diethyl phosphate + H2O
2-fluoro-4-nitrophenol + diethyl phosphate
-
-
-
?
2-methoxycarbonyl-1-methylvinyl dimethyl phosphate + H2O
?
i.e. mevinphos
-
-
?
2-methylpropyl 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl methylphosphonate + H2O
?
-
-
-
?
2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl N,N,N',N'-tetramethyldiamidophosphate + H2O
?
-
-
-
?
3,5-dinitrophenyl diethyl phosphate + H2O
3,5-dinitrophenol + diethyl phosphate
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl 1-methylethyl methylphosphonate + H2O
?
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl 2-methylpropyl methylphosphonate + H2O
?
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl cyclohexyl methylphosphonate + H2O
?
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl diethyl phosphate + H2O
?
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl dimethyl phosphate + H2O
?
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl ethyl methylphosphonate + H2O
3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one + ethyl methylphosphonate
3-cyanophenyl diethyl phosphate + H2O
3-cyanophenol + diethyl phosphate
-
-
-
?
3-fluoro-4-nitrophenyl diethyl phosphate + H2O
3-fluoro-4-nitrophenol + diethyl phosphate
-
-
-
?
3-fluorophenyl diethyl phosphate + H2O
3-fluorophenol + diethyl phosphate
-
-
-
?
3-nitrophenyl diethyl phosphate + H2O
3-nitrophenol + diethyl phosphate
-
-
-
?
3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O
?
i.e. soman
-
-
?
3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O
Pinacolyl methylphosphonate + fluoride
i.e. soman
-
-
?
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O
4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate
-
-
-
-
?
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O
4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate
-
-
-
-
?
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O
4-acetylphenol + 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate
-
-
-
-
?
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O
4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate
-
-
-
-
?
4-acetylphenyl (R)-2-methylpropyl methylphosphonate + H2O
(R)-2-methylpropyl methylphosphonate + 4-acetylphenol
-
-
-
?
4-acetylphenyl (S)-2-methylpropyl methylphosphonate + H2O
(S)-2-methylpropyl methylphosphonate + 4-acetylphenol
-
-
-
?
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate + H2O
4-acetylphenol + 2-methylpropyl (R)-methylphosphonate
-
-
-
-
?
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate + H2O
4-acetylphenol + 2-methylpropyl (S)-methylphosphonate
-
-
-
-
?
4-acetylphenyl cyclohexyl (R)-methylphosphonate + H2O
4-acetylphenol + cyclohexyl (R)-methylphosphonate
-
-
-
-
?
4-acetylphenyl cyclohexyl (S)-methylphosphonate + H2O
4-acetylphenol + cyclohexyl (S)-methylphosphonate
-
-
-
-
?
4-acetylphenyl cyclohexyl methyl (R)-phosphate + H2O
cyclohexyl methyl (R)-phosphate + 4-acetylphenol
-
-
-
?
4-acetylphenyl cyclohexyl methyl (S)-phosphate + H2O
cyclohexyl methyl (S)-phosphate + 4-acetylphenol
-
-
-
?
4-acetylphenyl ethyl (R)-methylphosphonate + H2O
4-acetylphenol + ethyl (R)-methylphosphonate
-
-
-
-
?
4-acetylphenyl ethyl (S)-methylphosphonate + H2O
4-acetylphenol + ethyl (S)-methylphosphonate
-
-
-
-
?
4-acetylphenyl propan-2-yl (R)-methylphosphonate + H2O
4-acetylphenol + propan-2-yl (R)-methylphosphonate
-
-
-
-
?
4-acetylphenyl propan-2-yl (S)-methylphosphonate + H2O
4-acetylphenol + propan-2-yl (S)-methylphosphonate
-
-
-
-
?
4-carbamoylphenyl diethyl phosphate + H2O
dibutyl phosphate + ?
-
-
-
?
4-chlorophenyl diethyl phosphate + H2O
4-chlorophenol + diethyl phosphate
-
-
-
?
4-cyanophenyl diethyl phosphate + H2O
4-cyanophenol + diethyl phosphate
-
-
-
?
4-diethyl phosphate acetophenone + H2O
4-hydroxyacetophenone + diethyl phosphate
-
-
-
?
4-diethyl phosphate benzaldehyde + H2O
4-hydroxybenzaldehyde + diethyl phosphate
-
-
-
?
4-diethyl phosphate methyl benzoate + H2O
methyl 4-hydroxybenzoate + diethyl phosphate
-
-
-
?
4-methyl-2-oxo-2H-chromen-7-yl 2-methylpropyl methylphosphonate + H2O
2-methylpropyl methylphosphonate + 7-hydroxy-4-methyl-2H-1-benzopyran-2-one
-
-
-
?
4-methyl-2-oxo-2H-chromen-7-yl N,N,N',N'-tetramethyldiamidophosphate + H2O
N,N,N',N'-tetramethylphosphorodiamidic acid + 7-hydroxy-4-methyl-2H-1-benzopyran-2-one
-
-
-
?
4-nitrophenyl diethyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
4-nitrophenyl phenyl methylphosphonate + H2O
4-nitrophenol + phenyl hydrogen methylphosphonate
4-nitrophenyl phenyl methylphosphonate + H2O
phenyl hydrogen methylphosphonate + 4-nitrophenol
-
wild-type, 78% of the activity with propyl 4-nitrophenyl methylphosphonate
-
-
?
4-nitrophenyl propan-2-yl methylphosphonate + H2O
propan-2-yl hydrogen methylphosphonate + 4-nitrophenol
-
wild-type, 57% of the activity with propyl 4-nitrophenyl methylphosphonate
-
-
?
4-nitrophenyl propyl methylphosphonate + H2O
4-nitrophenol + propyl hydrogen methylphosphonate
7-diethylphospho-6,8-difluor-4-methylumbelliferyl + H2O
diethylphosphate + 6,8-difluor-4-methylumbelliferol
-
high level of hydrolysis of the fluorogenic substrate, fluorescence assay method optimization
-
-
?
7-diethylphosphoro-3-cyanocoumarin + H2O
3-cyanocoumarin + diethyl phosphate
-
-
-
?
9-(2,4-dimethylphenoxycarbonyl)-10-methylacridinium triflate + H2O
?
synthesis method, overview. The assay is based on the PON1-mediated hydrolysis of an acridinium ester, and the hydrolysis is monitored by chemiluminescence decrease after incubation with PON enzyme
-
-
?
9-(4-chlorophenoxycarbonyl)-10-methylacridinium triflate + H2O
?
synthesis method, overview. The assay is based on the PON1-mediated hydrolysis of an acridinium ester, and the hydrolysis is monitored by chemiluminescence decrease after incubation with PON enzyme
-
-
?
9-(4-methylphenoxycarbonyl)-10-methylacridinium triflate + H2O
?
synthesis method, overview. The assay is based on the PON1-mediated hydrolysis of an acridinium ester, and the hydrolysis is monitored by chemiluminescence decrease after incubation with PON enzyme
-
-
?
9-(4-tert-butylphenoxycarbonyl)-10-methylacridinium triflate + H2O
?
synthesis method, overview. The assay is based on the PON1-mediated hydrolysis of an acridinium ester, and the hydrolysis is monitored by chemiluminescence decrease after incubation with PON enzyme
-
-
?
9-(phenyloxycarbonyl)-10-methylacridinium triflate + H2O
?
synthesis method, overview. The assay is based on the PON1-mediated hydrolysis of an acridinium ester, and the hydrolysis is monitored by chemiluminescence decrease after incubation with PON enzyme
-
-
?
bis(1-methylethyl) 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl phosphate + H2O
dipropan-2-yl phosphate + 7-hydroxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
-
-
-
?
bis(1-methylethyl) 4-methyl-2-oxo-2H-chromen-7-yl phosphate + H2O
dipropan-2-yl phosphate + 7-hydroxy-4-methyl-2H-1-benzopyran-2-one
-
-
-
?
butan-2-yl 4-nitrophenyl methylphosphonate + H2O
butan-2-yl hydrogen methylphosphonate + 4-nitrophenol
-
wild-type, 50% of the activity with propyl 4-nitrophenyl methylphosphonate
-
-
?
chlorpyrifos-oxon + H2O
3,5,6-trichloro-2-pyridinol + diethyl phosphate
-
-
-
?
chlorpyrifosoxon + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl phosphate
-
-
-
?
chlortion + H2O
?
chlorthion is O,O-dimethyl O-(3-chloro-4-nitrophenyl) thionophosphate
-
-
?
coumaphos + H2O
O,O-diethylphosphorothioate + 3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one
cf. EC 3.1.8.2
-
-
?
cyclohexyl 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl methylphosphonate + H2O
cyclohexyl methylphosphonate + 7-hydroxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
-
-
-
?
cyclohexyl 4-methyl-2-oxo-2H-chromen-7-yl methylphosphonate + H2O
cyclohexyl methylphosphonate + 7-hydroxy-4-methyl-2H-1-benzopyran-2-one
-
-
-
?
cyclohexyl ethyl 4-nitrophenyl (R)-phosphate + H2O
cyclohexyl ethyl (R)-phosphate + 4-nitrophenol
-
-
-
?
cyclohexyl ethyl 4-nitrophenyl (S)-phosphate + H2O
cyclohexyl ethyl (S)-phosphate + 4-nitrophenol
-
-
-
?
cyclohexyl methyl 4-nitrophenyl (R)-phosphate + H2O
cyclohexyl methyl (R)-phosphate + 4-nitrophenol
-
-
-
?
cyclohexyl methyl 4-nitrophenyl (S)-phosphate + H2O
cyclohexyl methyl (S)-phosphate + 4-nitrophenol
-
-
-
?
cyclohexyl methyl phenyl (R)-phosphate + H2O
cyclohexyl methyl (R)-phosphate + phenol
-
-
-
?
cyclohexyl methyl phenyl (S)-phosphate + H2O
cyclohexyl methyl (S)-phosphate + phenol
-
-
-
?
cyclohexylmethylphosphonofluoridate + H2O
cyclohexyl methylphosphonate + fluoride
i.e. cyclosarin
-
-
?
demeton-S methyl + H2O
2-ethylsulfanyl-ethanethiol + dimethyl phosphate
-
-
-
-
?
demeton-S-methyl + H2O
2-ethylsulfanyl-ethanethiol + dimethyl phosphate
-
-
-
-
?
diazoxon + H2O
6-methyl-2-(1-methylethyl)pyrimidin-4-ol + diethyl phosphate
-
-
-
-
ir
dibutyl 4-acetophenyl phosphate + H2O
dibutyl phosphate + 4-hydroxyacetophenone
-
-
-
?
dibutyl 4-nitrophenyl phosphate + H2O
dibutyl phosphate + 4-nitrophenol
-
-
-
?
dibutyl phenyl phosphate + H2O
dibutyl phosphate + phenol
-
-
-
?
dichlorvos + H2O
2,2-dichloroethenol + dimethyl hydrogen phosphate
-
-
-
?
diethyl 2,4,6-trifluorophenyl phosphate + H2O
diethyl phosphate + 2,4,6-trifluorophenol
-
-
-
?
diethyl 2,4-difluorophenyl phosphate + H2O
diethyl phosphate + 2,4-difluorophenol
-
-
-
?
diethyl 2,6-difluoro-4-nitrophenyl phosphate + H2O
diethyl phosphate + 2,6-difluoro-4-nitrophenol
-
-
-
?
diethyl 2-(dimethoxyphosphorylsulfanyl)butanedioate + H2O
?
i.e. malaoxon
-
-
?
diethyl 2-fluoro-4-nitrophenyl phosphate + H2O
diethyl phosphate + 2-fluoro-4-nitrophenol
-
-
-
?
diethyl 2-fluorophenyl phosphate + H2O
diethyl phosphate + 2-fluorophenol
-
-
-
?
diethyl 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl phosphate + H2O
diethyl phosphate + 7-hydroxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
-
-
-
?
diethyl 3-fluoro-4-nitrophenyl phosphate + H2O
diethyl phosphate + 3-fluoro-4-nitrophenol
best substrate
-
-
?
diethyl 3-fluorophenyl phosphate + H2O
diethyl phosphate + 4-fluorophenol
high activity
-
-
?
diethyl 4-acetophenyl phosphate + H2O
diethyl phosphate + 4-hydroxyacetophenone
-
-
-
?
diethyl 4-chlorophenyl phosphate + H2O
diethyl phosphate + 4-chlorophenol
-
-
-
?
diethyl 4-chlorophenyl thiophosphate + H2O
4-chlorophenol + diethyl thiophosphate
-
-
-
?
diethyl 4-cyanophenyl phosphate + H2O
diethyl phosphate + 4-hydroxybenzonitril
-
-
-
?
diethyl 4-fluorophenyl phosphate + H2O
diethyl phosphate + 4-fluorophenol
-
-
-
?
diethyl 4-formylphenyl phosphate + H2O
diethyl phosphate + 4-formylphenol
-
-
-
?
diethyl 4-methoxyphenyl phosphate + H2O
diethyl phosphate + 4-methoxyphenol
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
p-nitrophenol + diethyl phosphate
-
-
-
-
?
diethyl pentafluoro-phenyl phosphate + H2O
diethyl phosphate + 2,3,4,5,6-pentafluorophenol
high activity
-
-
?
diethyl phenyl phosphate + H2O
diethyl phosphate + phenol
-
-
-
?
diethylumbelliferyl phosphate + H2O
diethylumbelliferol + phosphate
-
-
-
-
?
dimethyl 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl phosphate + H2O
dimethyl phosphate + 7-hydroxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
-
-
-
?
dimethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + dimethyl hydrogen phosphate
13.3% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
dimethyl 4-nitrophenyl phosphate + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
?
dyfonate + H2O
O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol
-
-
-
-
?
ethyl 1-methylethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + ethyl 1-methylethyl hydrogen phosphate
ethyl 2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl methylphosphonate + H2O
ethyl methylphosphonate + 7-hydroxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one
-
-
-
?
ethyl 4-methyl-2-oxo-2H-chromen-7-yl methylphosphonate + H2O
ethyl methylphosphonate + 7-hydroxy-4-methyl-2H-1-benzopyran-2-one
-
-
-
?
ethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O
4-nitrophenol + ethyl hydrogen (1-methylpropyl)phosphonate
ethyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + ethyl hydrogen methylphosphonate
ethyl 4-nitrophenyl methylphosphonate + H2O
ethyl hydrogen methylphosphonate + 4-nitrophenol
-
wild-type, 50% of the activity with propyl 4-nitrophenyl methylphosphonate
-
-
?
ethyl-paraoxon + H2O
?
the enzyme is a poor phosphotriesterase
-
-
?
fenitrothion + H2O
O,O-diethylphosphorothioate + 3-methyl-4-nitrophenol
-
-
-
?
fensulfothion + H2O
O,O-diethylphosphorothioate + 4-(methylsulfinyl)phenol
some enzyme mutants are also capable of degrading fensulfothion, which is reported to be an inhibitor for the wild-type enzyme, as well as others that are not substrates of the starting template or previously reported W263 mutants
-
-
?
isopropyl methylphosphonofluoridate + H2O
propan-2-yl methylphosphonate + HF
-
-
-
-
?
malathion + H2O
O,O-diethylphosphorothioate + diethyl 2-mercaptosuccinate
low activity
-
-
?
methyl 1-methylethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + methyl 1-methylethyl hydrogen phosphate
100% activity
-
-
?
methyl 4-nitrophenyl methylphosphonate + H2O
methyl hydrogen methylphosphonate + 4-nitrophenol
-
wild-type, 733% of the activity with propyl 4-nitrophenyl methylphosphonate
-
-
?
methyl 4-nitrophenyl phenyl (R)-phosphate + H2O
methyl phenyl (R)-phosphate + 4-nitrophenol
-
-
-
?
methyl 4-nitrophenyl phenyl (S)-phosphate + H2O
methyl phenyl (S)-phosphate + 4-nitrophenol
-
-
-
?
methyl 4-nitrophenyl propan-2-yl (R)-phosphate + H2O
methyl propan-2-yl (R)-phosphate + 4-nitrophenol
-
-
-
?
methyl 4-nitrophenyl propan-2-yl (S)-phosphate + H2O
methyl propan-2-yl (S)-phosphate + 4-nitrophenol
-
-
-
?
methyl 4-[(diethoxyphosphoryl)oxy]benzoate + H2O
dibutyl phosphate + ?
-
-
-
?
methyl bis(4-nitrophenyl) phosphate + H2O
methyl phosphate + 2 4-nitrophenol
-
-
-
?
methyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
methylphosphonic acid + H2O
?
i.e. MPA, according to the C-P lyase pathway, methylphosphonic acid decomposition by the enzyme is expected to yield methane as a product. But no methane is detected during the reaction process. Methanol cannot be detected either during the MPA decomposition reaction, as well as formaldehyde, but formic acid is identified in the reaction mixture
-
-
?
mono(diethylphosphoryl)obidoxime + H2O
diethyl hydrogenphosphate + obidoxime
-
substrate is a potent inhibitor of human acetylcholinesterase
-
-
?
N,N-diethyl-2-(methyl-(2-methylpropoxy)phosphoryl)sulfanylethanamine + H2O
?
i.e. VR
-
-
?
N-[2-[ethoxy(methyl)phosphoryl]sulfanethyl]-N-propan-2-ylpropan-2-amine + H2O
S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
the enzyme shows only minimal activity against the nerve agent VX
-
-
?
nitrophenyl isopropyl methylphosphonate + H2O
nitrophenol + propan-2-yl hydrogen methylphosphonate
-
a series of substituted phenoxyalkyl pyridinium oximes enhance the degradation of surrogates of sarin (i.e. nitrophenyl isopropyl methylphosphonate, NIMP) and VX (i.e. nitrophenyl ethyl methylphosphonate, NEMP). Neither NIMP nor NEMP is hydrolyzed effectively by paraoxonase PON1 if one of these oximes is absent. In the presence of eight novel oximes, PON1-mediated degradation of both surrogates occurs
-
-
?
O,O,S-tributyl phosphorothioate + H2O
dibutyl phosphate + ?
-
-
-
?
O,O-diethyl fluorophosphate + H2O
diethylphosphate + fluoride
-
activity of EC 3.1.8.2
-
-
?
O,O-diethyl O-4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
O,O-diethyl O-[4-methyl-6-(propan-2-yl)pyrimidin-2-yl] phosphorothioate + H2O
O,O-diethyl phosphorothioate + 4-methyl-6-(propan-2-yl)pyrimidin-2-ol
-
i.e. diazinon
-
-
?
O,O-diethyl-S-[2-diethylaminoethyl]thiophosphate + H2O
?
i.e. amiton or VG
-
-
?
O,O-diethyl-S-[2-diisopropylaminoethyl]thiophosphate + H2O
?
i.e. diisopropyl-amiton
-
-
?
O,O-diethyl-S-[2-dimethylaminoethyl]thiophosphate + H2O
?
i.e. dimethyl-amiton
-
-
?
O,O-diisopropyl-4-nitrophenyl phosphate + H2O
dipropan-2-yl hydrogen phosphate + 4-nitrophenol
-
-
-
-
?
O,O-dimethyl O-(4-methyl-2-oxo-2H-chromen-7-yl) thiophosphate + H2O
?
-
-
-
?
O,O-dimethyl O-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl] thiophosphate + H2O
?
-
-
-
?
O-ethyl S-(2-diisopropylaminoethyl) methylphosphonothioate + H2O
?
-
i.e. VX, a highly toxic organophosphorus nerve agent, stereospecific hydrolysis
-
-
?
O-ethyl S-(2-diisopropylaminoethyl)methylphosphonothioate + H2O
?
also called VX
-
-
?
O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate + H2O
?
-
-
-
?
O-ethyl-S-(2-di-n-propylaminoethyl)methylphosphonothiolate + H2O
?
i.e. n-propyl-VX
-
-
?
O-ethyl-S-(2-diethylaminoethyl)ethylphosphonothiolate + H2O
?
i.e. VE
-
-
?
O-ethyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VM
-
-
?
O-ethyl-S-(2-diisopropylaminoethyl)ethylphosphonothiolate + H2O
?
i.e. isopropyl-VE
-
-
?
O-ethyl-S-(2-dimethylaminoethyl)ethylphosphonothiolate + H2O
?
i.e. methyl-VE
-
-
?
O-ethyl-S-(2-dimethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. dimethyl-VX
-
-
?
O-ethyl-S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + H2O
S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
-
hydrolysis is exclusively preferential for the P+ isomer. Glycosylation state of PON1 does not affect substrate stereoselectivity
-
-
?
O-ethyl-S-[2-(diisopropylamino)ethyl]methylphosphonothioate + H2O
S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
-
O-ethyl-S-[2-(diisopropylamino)ethyl]methylphosphonothiate's lone oxygen atom has a strong preference for forming a direct electrostatic interaction with PON1's active site calcium ion. Key residues, which interact with VX are E53, H115, N168, F222, N224, L240, D269, I291, F292, and V346. Residue D183 located in PON1's active site may act as a proton donor or accepter during hydrolysis. PON1's flexible loop region acts as a gatekeeper to the active site residues required for binding VX
-
-
?
O-isobutyl S-(2-N,N-diethylaminoethyl)methylphosphonothioate + H2O
?
also called VR
-
-
?
O-isobutyl-S-[2-(diethylamino)ethyl]methylphosphonothioic acid + H2O
?
-
hydrolysis is exclusively preferential for the P+ isomer. Glycosylation state of PON1 does not affect substrate stereoselectivity
-
-
?
O-methyl O-(4-nitrophenyl) methylphosphonothioate + H2O
O-methyl-methylphosphonothionic acid + 4-nitrophenol
-
-
-
-
?
O-methyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. methyl-VM
-
-
?
O-n-butyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. CVX or Chinese VX
-
-
?
pentafluorophenyl diethyl phosphate + H2O
pentafluorophenol + diethyl phosphate
-
-
-
?
phosmet + H2O
N-hydroxymethyl-phthalimide + O,O-dimethyl dithiophosphate
-
-
-
-
?
propyl 4-nitrophenyl methylphosphonate + H2O
propyl hydrogen methylphosphonate + 4-nitrophenol
-
-
-
-
?
RP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O
?
-
-
-
-
?
RP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O
?
-
-
-
-
?
russian VX + H2O
methyl-phosphonothioic acid S-(2-diethylamino-ethyl) ester + 2-methylpropanol
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate
O-ethyl hydrogen methylphosphonothioate + 2-[di(propan-2-yl)amino]ethanol
-
i.e. nerve agent VX
-
-
?
SP-O-ethyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O
?
-
-
-
-
?
SP-O-n-propyl methylphosphonyl-3-cyano-7-hydroxy-4-methylcoumarin + H2O
?
-
-
-
-
?
(+)-cyclosarin + H2O
methyl-phosphonic acid monofluoride + cyclohexanol
wild type enzyme and mutant enzyme W263F hydrolyze the (+)-enantiomer approximately 3 and 4.5 times faster than the (-)-enantiomer
-
-
?
(+)-cyclosarin + H2O
methyl-phosphonic acid monofluoride + cyclohexanol
wild type enzyme and mutant enzyme W263F hydrolyze the (+)-enantiomer approximately 3 and 4.5 times faster than the (-)-enantiomer
-
-
?
1-methylethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O
4-nitrophenol + 1-methylethyl hydrogen (1-methylpropyl)phosphonate
6.8% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
1-methylethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O
4-nitrophenol + 1-methylethyl hydrogen (1-methylpropyl)phosphonate
low activity
-
-
?
1-methylethyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + 1-methylethyl hydrogen methylphosphonate
33.1% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
1-methylethyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + 1-methylethyl hydrogen methylphosphonate
-
-
-
?
1-methylpropyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + 1-methylpropyl hydrogen methylphosphonate
29.5% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
1-methylpropyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + 1-methylpropyl hydrogen methylphosphonate
best substrate
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl ethyl methylphosphonate + H2O
3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one + ethyl methylphosphonate
-
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl ethyl methylphosphonate + H2O
3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one + ethyl methylphosphonate
-
-
-
?
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
-
-
-
?
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
-
-
-
?
4-nitrophenyl phenyl methylphosphonate + H2O
4-nitrophenol + phenyl hydrogen methylphosphonate
45.2% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
4-nitrophenyl phenyl methylphosphonate + H2O
4-nitrophenol + phenyl hydrogen methylphosphonate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl propyl methylphosphonate + H2O
4-nitrophenol + propyl hydrogen methylphosphonate
58.5% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
4-nitrophenyl propyl methylphosphonate + H2O
4-nitrophenol + propyl hydrogen methylphosphonate
best substrate
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
a popular pesticide used in agricultural crop protection causes widespread contamination, degradation by evolutionary improved genetic OPH variants, e.g. variants B3561 and B1368, overview
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
the wild-type enzyme shows 1000fold lower activity compared to substrate paraoxon, analysis of activity with chlorpyrifos of genetic variants, 22A11, A1030, A1467, B1368, B2136, and B3561, the wild-type shows extremely low activity while variants B3561 and B1368 show high activity, overview
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
-
the wild-type enzyme shows lower activity compared to the activity of genetic variant 22A11, overview
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
-
the wild-type enzyme shows lower activity compared to the activity of genetic variant 22A11, overview
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
a popular pesticide used in agricultural crop protection causes widespread contamination, degradation by evolutionary improved genetic OPH variants, e.g. variants B3561 and B1368, overview
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
the wild-type enzyme shows 1000fold lower activity compared to substrate paraoxon, analysis of activity with chlorpyrifos of genetic variants, 22A11, A1030, A1467, B1368, B2136, and B3561, the wild-type shows extremely low activity while variants B3561 and B1368 show high activity, overview
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
-
-
-
?
chlorpyrifos + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl thiophosphate
-
-
-
-
?
chlorpyrifos + H2O
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
-
-
-
-
?
chlorpyrifos + H2O
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
Latilactobacillus sakei WCP904
-
-
-
-
?
chlorpyrifos + H2O
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol
low activity, cf. EC 3.1.8.2
-
-
?
chlorpyrifos oxon + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl phosphate
-
-
-
?
chlorpyrifos oxon + H2O
3,5,6-trichloro-pyridin-2-ol + diethyl phosphate
-
-
-
-
?
chlorpyrifos oxon + H2O
diethyl phosphate + 3,5,6-trichloropyridin-2-ol
-
-
-
?
chlorpyrifos oxon + H2O
diethyl phosphate + 3,5,6-trichloropyridin-2-ol
i.e. CPO, a metabolite of chlorpyrifos that is used as a pesticide in agriculture industry
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
Nocardia asteroides ATCC 19296
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
Pseudarthrobacter oxydans ATCC 14359
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
Streptomyces phaeochromogenes CCRC 10811
-
substrate is used for in vitro activity detection
-
-
?
coumaphos + H2O
3-chloro-4-methylumbelliferone + diethyl thiophosphate
-
-
-
-
?
coumaphos + H2O
3-chloro-4-methylumbelliferone + diethyl thiophosphate
-
-
-
?
coumaphos + H2O
3-chloro-4-methylumbelliferone + diethyl thiophosphate
-
-
-
-
?
coumaphos + H2O
O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone
-
-
-
-
?
coumaphos + H2O
O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone
Latilactobacillus sakei WCP904
-
-
-
-
?
CVX + H2O
?
-
i.e. diethylamino-ethyl-O-butyl methylphosphonothioate
-
-
?
cyclohexylmethylphosphonofluoridate + H2O
?
i.e. cyclosarin, activity of 3.1.8.2
-
-
?
cyclohexylmethylphosphonofluoridate + H2O
?
i.e. cyclosarin
-
-
?
cyclohexylmethylphosphonofluoridate + H2O
?
i.e. cyclosarin
-
-
?
cyclosarin + H2O
methyl-phosphonic acid monofluoride + cyclohexanol
-
-
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
-
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
-
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
-
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
0.2 mM, 26% degradation over 24 h by whole cells
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
0.2 mM, 26% degradation over 24 h by whole cells
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
0.2 mM, 27% degradation over 24 h by whole cells
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
0.2 mM, 27% degradation over 24 h by whole cells
-
-
?
diazinon + H2O
?
-
0.2 mM, 24% degradation over 24 h by whole cells
-
-
?
diazoxon + H2O
2-isopropyl-6-methyl-pyrimidin-4-ol + diethyl phosphate
-
-
-
?
diazoxon + H2O
2-isopropyl-6-methyl-pyrimidin-4-ol + diethyl phosphate
the homozygote wild type enzyme (QQ phenotype of Q192R) hydrolyzes diaxozon more rapidly than paraoxon
-
-
?
diazoxon + H2O
2-isopropyl-6-methyl-pyrimidin-4-ol + diethyl phosphate
the homozygote wild type enzyme shows highest activity towards diazoxon
-
-
?
diazoxon + H2O
2-isopropyl-6-methyl-pyrimidin-4-ol + diethyl phosphate
-
-
-
-
?
dibutyl 2,2,3,3-tetrafluorobutyl phosphate + H2O
dibutyl phosphate + ?
-
-
-
?
dibutyl 2,2,3,3-tetrafluorobutyl phosphate + H2O
dibutyl phosphate + ?
low activity
-
-
?
dibutyl 3,3-difluorobutyl phosphate + H2O
dibutyl phosphate + ?
very low activity
-
-
?
dibutyl 3,3-difluorobutyl phosphate + H2O
dibutyl phosphate + ?
low activity
-
-
?
diethyl (3,5,6-trichloropyridin-2-yl) phosphate + H2O
?
i.e. chlorpyrifos-oxon
-
-
?
diethyl (3,5,6-trichloropyridin-2-yl) phosphate + H2O
?
i.e. chlorpyrifos-oxon or CPO
-
-
?
diethyl 4-chlorophenyl phosphate + H2O
4-chlorophenol + diethyl phosphate
-
-
-
?
diethyl 4-chlorophenyl phosphate + H2O
4-chlorophenol + diethyl phosphate
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl hydrogen phosphate
15.1% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl hydrogen phosphate
low activity
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl hydrogen phosphate
i.e. paraoxon
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl hydrogen phosphate
i.e. paraoxon
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
both docking and molecular dynamics simulations suggest that the only way paraoxon can be accommodated in the PON1 active site is by pushing Y71 out of the active site, causing the active site loop to take on a partially open conformation
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
diethyl-parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
diethyl-parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
?
diethyl-parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
diethyl-parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
?
diethyl-parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
diisopropyl fluorophosphate + H2O
?
-
high concentration of purified human serum paraoxonase 1 (about 0.075 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates
-
-
?
diisopropyl fluorophosphate + H2O
?
-
high concentration of purified rabbit serum paraoxonase 1 (about 0.025 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates
-
-
?
diisopropyl fluorophosphate + H2O
diisopropyl phosphate + fluoride
reaction of EC 3.1.8.2
-
-
?
diisopropyl fluorophosphate + H2O
diisopropyl phosphate + fluoride
reaction of EC 3.1.8.2, highly toxic structural analogue of G-class type of nerve agents
-
-
?
dimethoate + H2O
?
-
0.2 mM, 14% degradation over 24 h by whole cells
-
-
?
dimethoate + H2O
?
-
0.2 mM, 14% degradation over 24 h by whole cells
-
-
?
dimethoate + H2O
?
-
0.2 mM, 16% degradation over 24 h by whole cells
-
-
?
dimethoate + H2O
?
-
0.2 mM, 16% degradation over 24 h by whole cells
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Nocardia asteroides ATCC 19296
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Nocardia asteroides ATCC 19296
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Pseudarthrobacter oxydans ATCC 14359
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Pseudarthrobacter oxydans ATCC 14359
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Pseudarthrobacter oxydans ATCC 14359
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Pseudarthrobacter oxydans ATCC 14359
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Streptomyces phaeochromogenes CCRC 10811
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Streptomyces phaeochromogenes CCRC 10811
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Streptomyces phaeochromogenes CCRC 10811
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
Streptomyces phaeochromogenes CCRC 10811
-
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
ethyl 1-methylethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + ethyl 1-methylethyl hydrogen phosphate
18.8% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
ethyl 1-methylethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + ethyl 1-methylethyl hydrogen phosphate
low activity
-
-
?
ethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O
4-nitrophenol + ethyl hydrogen (1-methylpropyl)phosphonate
14.7% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
ethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O
4-nitrophenol + ethyl hydrogen (1-methylpropyl)phosphonate
-
-
-
?
ethyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + ethyl hydrogen methylphosphonate
29.8% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate
-
-
?
ethyl 4-nitrophenyl methylphosphonate + H2O
4-nitrophenol + ethyl hydrogen methylphosphonate
low activity
-
-
?
ethyl dimethylphosphoramidocyanidate + H2O
?
i.e. tabun
-
-
?
ethyl paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
ethyl paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
-
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
-
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
0.2 mM, 89% degradation over 24 h by whole cells
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
0.2 mM, 89% degradation over 24 h by whole cells
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
0.2 mM, 95% degradation over 24 h by whole cells
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
0.2 mM, 95% degradation over 24 h by whole cells
-
-
?
malathion + H2O
O,O-diethyl phosphorothioate + diethyl 2-mercaptosuccinate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
O,O-dimethyl O-4-nitrophenyl phosphate
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethylphosphate
-
the enzyme reaction also produces two protons, which lower the pH and establish a steady pH gradient
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethylphosphate
-
the enzyme from Sulfolobus solfataricus has a low paraoxonase activity
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethylphosphate
Saccharolobus solfataricus MT-4 / DSM 5833
-
the enzyme from Sulfolobus solfataricus has a low paraoxonase activity
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethylphosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
i.e. O,O-dimethyl O-4-nitrophenyl phosphorothioate
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
i.e. O,O-dimethyl O-4-nitrophenyl phosphorothioate
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VX
-
-
?
O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VX
-
-
?
O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VX
-
-
?
O-isobutyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VR or Russian VX
-
-
?
O-isobutyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. RVX, VR, or Russian VX
-
-
?
O-isopropyl methylphosphonofluoridate + H2O
?
i.e. sarin
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
the enzyme prevents, independent of compound aging, the inhibition of acetylcholinesterase by paraoxon, and also by VX, sarin and soman, the latter are phosphyloxime products of acetlycholinesterase reaction with organophosphate inhibitors, overview
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
preferred substrate
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
652704, 664153, 665987, 702421, 707155, 707214, 708095, 709262, 709264, 709291, 709755, 714112, 714893, 714895, 715738, 715744, 716957, 728992, 750345, 750484, 750511, 752173
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
enzyme variant Q192: 1.3% of the activity with phenyl acetate (see EC 3.1.1.2), enzyme variant R192: 1% of the activity with phenyl acetate (see EC 3.1.1.2). Enzyme protein also shows activities of EC 3.1.1.25 and EC 3.1.1.2
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
human serum paraoxonase/arylesterase is an esterase with broad substrate specificity. It occurs in two genetically determined allozymic forms, type A and type B. These allozymes are the products of two allelic genes located at the paraoxonase locus on chromosome 7, which is closely linked to the gene for cystic fibrosis. Paraoxonase activity of the B type isozyme is considerably higher and stimulated more by 1 M NaCl than A-type paraoxonase. The ratio of paraoxonase activity to arylesterase activity of the B-isozyme is about 8, and that of the A isozyme about 1
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
i.e. O,O'-diethyl-4-nitrophenyl phosphothioate
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
0.2 mM, 12% degradation over 24 h by whole cells
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
0.2 mM, 12% degradation over 24 h by whole cells
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
0.2 mM, 35% degradation over 24 h by whole cells
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
0.2 mM, 35% degradation over 24 h by whole cells
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
the enzyme from Sulfolobus solfataricus has a low paraoxonase activity
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
Saccharolobus solfataricus MT-4 / DSM 5833
-
the enzyme from Sulfolobus solfataricus has a low paraoxonase activity
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
i.e. diethyl-4-nitrophenyl-phosphate
-
-
?
paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
paraoxon is diethyl 4-nitrophenyl phosphate
-
-
?
paraoxon + H2O
diethyl phosphate + 4-nitrophenol
high activity with enzyme mutant C258L/I261F/W263A
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenyl phosphate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenyl phosphate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
paraoxonase and arylesterase activities are significantly lower in a Helicobacter pylori positive group than in a Helicobacter pylori negative group, overview
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenylphosphate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
A-type paraoxonase shows 0.06% of the activity with phenyl acetate, B-type paraoxonase shows 2.5% of the activity with phenyl acetate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
higher activity towards paraoxon than towards phenyl acetate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
paraoxonase activity of PON1 is polymorphism dependent
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
PON1 activity determined towards the synthetic compounds paraoxon and phenyl acetate reflects no association with markers of oxidative stress
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
the enzyme exists in two genetically determined allozymic forms, and these A and B allozymes possess both paraoxonase and arylesterase activities. B-type esterase has relatively higher paraoxonase activity and is stimulated to a greater degree by 1 M NaCl than the A allozyme
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
the homozygote RR phenotype of Q192R shows highest activity towards paraoxon
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
0.2% of the activity with phenyl acetate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
paraoxon is the best substrate for the purified arylesterase
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
-
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
engneered mutant strain, parathion metabolism, overview
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
engneered mutant strain, parathion metabolism, overview
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
-
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
-
-
?
parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
?
parathion + H2O
diethylthiophosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenyl thiophosphate
-
-
?
parathion + H2O
diethylthiophosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenyl thiophosphate
-
-
?
russian VX + H2O
methyl-phosphonothioic acid S-(2-diethylamino-ethyl) ester + 2-methylpropanol
-
-
-
-
?
russian VX + H2O
methyl-phosphonothioic acid S-(2-diethylamino-ethyl) ester + 2-methylpropanol
-
-
-
-
?
sarin + H2O
?
-
efficient hydrolysis of sarin (11 nM) is observed with about 0.08-0.25 units of paraoxonase 1
-
-
?
sarin + H2O
?
-
efficient hydrolysis of sarin (11 nM) is observed with about 0.08-0.25 units of paraoxonase 1
-
-
?
sarin + H2O
methyl-phosphonic acid monofluoride + isopropyl alcohol
-
-
-
-
?
sarin + H2O
methyl-phosphonic acid monofluoride + isopropyl alcohol
PON1 hydrolyzes sarin more effectively than paraoxon, sarin is o-isopropyl methylphosphonofluoridate
-
-
?
sarin + H2O
methylphosphonofluoride acid + ?
-
a highly toxic organophosphorus nerve agent, stereospecific hydrolysis
-
-
?
sarin + H2O
methylphosphonofluoride acid + ?
-
a highly toxic organophosphorus nerve agent
-
-
?
soman + H2O
?
-
efficient hydrolysis of soman (3 nM) is observed with about 0.08-0.25 units of paraoxonase 1
-
-
?
soman + H2O
?
-
efficient hydrolysis of soman (3 nM) is observed with about 0.08-0.25 units of paraoxonase 1
-
-
?
soman + H2O
methyl-phosphonic acid monofluoride + 1,2,2-trimethylpropanol
-
-
-
-
?
soman + H2O
methyl-phosphonic acid monofluoride + 1,2,2-trimethylpropanol
-
-
-
-
?
soman + H2O
methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol
-
i.e. pinacolyl methylphosphonofluoridate, a highly toxic organophosphorus nerve agent, stereospecific hydrolysis
-
-
?
soman + H2O
methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol
PON1 hydrolyzes soman more effectively than paraoxon, soman is o-pinacolyl methylphosphonofluoridate
-
-
?
soman + H2O
methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol
-
i.e. pinacolyl methylphosphonofluoridate, a highly toxic organophosphorus nerve agent
-
-
?
SP-CMP + H2O
?
SP-CMP is the toxic SP enantiomer of a cyclosarin surrogate in which the fluoride leaving group is replaced by a coumarin derivative
-
-
?
SP-CMP + H2O
?
SP-CMP is the toxic SP enantiomer of a GF surrogate in which the fluoride leaving group is replaced by a coumarin derivative
-
-
?
tabun + H2O
?
-
efficient hydrolysis of tabun (100 nM) is observed with about 0.025-0.04 units of paraoxonase 1. Tabun hydrolysis with paraoxonase 1 is about 30-60times and about 200-260times more efficient than that with sarin and soman, respectively
-
-
?
tabun + H2O
?
-
efficient hydrolysis of tabun (100 nM) is observed with about 0.025-0.04 units of paraoxonase 1. Tabun hydrolysis with paraoxonase 1 is about 30-60times and about 200-260times more efficient than that with sarin and soman, respectively
-
-
?
VR + H2O
?
-
i.e. diethylamino-ethyl-O-isobutyl methylphosphonothioate
-
-
?
VX + H2O
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i.e. diisopropylamino-ethyl-O-ethyl methylphosphonothioate
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VX + H2O
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i.e. O-ethyl S-[2-diisopropylaminoethyl]methylphosphonothiolate
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VX + H2O
S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
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VX + H2O
S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
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additional information
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substrate binding modes and structure, overview
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additional information
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substrate binding modes and structure, overview
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additional information
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the enzyme is involved in detoxification of a broad range of organophosphorus pesticides and chemical warfare agents sarin and VX
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additional information
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OPH catalyzes hydrolysis of organophosphorus compounds, OPC15-20, to give phosphoric or alkylphosphonic acid derivatives, which is accompanied by a decrease in the pH of the reaction medium through release of two protons
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additional information
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the enzyme also exhibits activity of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview
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additional information
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the enzyme also exhibits activity of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview
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additional information
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wild-type PTE exhibits little stereoselectivity against the relatively small phosphonate center of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, but the elevated pKa of the thiol-leaving group provides a significant challenge for enzyme-catalyzed hydrolysis. Wild-type PTE has enzymatic activity for the hydrolysis of racemic VR similar to VX, but the larger isobutyl group attached to the phosphorus center results in a 25fold preference for the RP-enantiomer. Computational docking study of wild-type and mutant enzymes with the substrates
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additional information
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the enzyme does not hydrolyse 4-nitrophenyl phenyl ethylphosphonate and 1-methylpropyl 4-nitrophenyl ethylphosphonate
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?
additional information
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substrate specificities of wild-type enzyme and genetic variants, overview
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?
additional information
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substrate specificity of wild-type and genetic variants, overview
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?
additional information
?
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substrate specificities of wild-type enzyme and genetic variants, overview
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?
additional information
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substrate specificity of wild-type and genetic variants, overview
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additional information
?
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isozyme PON2 has antioxidant properties, prevents low density lipoprotein lipid peroxidation and reverses the oxidation of mildly oxidized low density lipoproteins
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additional information
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PON1 is involved in metabolism of oxidized lipid compounds
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additional information
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isozyme PON1 is effective at reducing the activity of phospholipid oxidation products
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additional information
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the enzyme PON2 has antioxidant properties, prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of mildly oxidized LDL to induce monocyte chemotaxis
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?
additional information
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HDL-associated enzyme is a cardio- and vasoprotective enzyme
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additional information
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the enzyme associated to high-density lipoproteins HDL exhibits antioxidant function of particular physiological relevance, mechanism, compositional fluctuations of HDL effects the influence of the enzyme on cardiovascular risks, overview, PON1 content is reduced in human ApoAI deficiency disease
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additional information
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the enzyme efficiently hydrolyzes organophosphorus nerve agents
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additional information
?
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the enzyme is important in detoxification of organophosphorus compounds, structure and applications of organophosphorus compounds, clinical evidence of PON1, overview
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additional information
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the serum paraoxonase family contains detoxifying and anti-atherosclerotic enzymes
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additional information
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enzyme-HDL complex: interaction and structure analysis
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additional information
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no activity with dimefox, parathion, methyl parathion, and diisopropyl fluorophosphate, assay method evaluation of analogues with fluorescent leaving groups for screening and selection of the enzyme that efficiently hydrolyzes organophosphorus nerve agents, overview
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?
additional information
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the enzyme associates to HDL in the blood stream
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additional information
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polymorphisms allele frequency in relation to exposure of individuals to organophosphates, overview
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additional information
?
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polymorphisms allele frequency in relation to exposure of individuals to organophosphates, overview
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additional information
?
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PON1 has a protective role e.g. against organophosphorous poisoning, but also against vascular diseases, PON1 has an anti-atherogenic activity
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additional information
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PON1 hydrolyses esters, including organophosphates and lactones, and exhibits anti-atherogenic properties, the enzyme is associated to high-density-lipoprotein, bound together with the human phosphate binding protein
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additional information
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PON1 is a high-density lipoprotein-associated enzyme capable of hydrolyzing diverse substrates from organophosphate toxins to oxidized phospholipids, PON1 is linked with both the prevention of organophosphate poisoning and inhibition of atherosclerosis initiated by oxidatively modified low-density lipoprotein, overview, phenotypic distribution of the enzyme in individuals of both sexes and different age, overview
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additional information
?
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the enzyme depends on high-density lipoprotein HDL as carrier and site of action, PON1 acts as an antioxidant preventing low-density lipoprotein peroxidation, overview
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additional information
?
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PON1 is a high-density lipoprotein-associated enzyme capable of hydrolyzing diverse substrates from organophosphate toxins to oxidized phospholipids
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additional information
?
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PON1 is associated to HDL and human phosphate-binding protein
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additional information
?
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residues Phe222 and His115 are important in organophosphorus substrate specificity and catalysis
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?
additional information
?
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high-density lipoprotein-associated paraoxonase possesses peroxidase-like activity that can contribute to the protective effect of paraoxonase against lipoprotein oxidation. The presence of paraoxonase in high-density lipoprotein may thus be a major contributor to the antiatherogenicity of this lipoprotein
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additional information
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PON1 hydrolyzes organophosphates, such as paraoxon, aromatic esters, for instance, phenyl acetate, and also lipid peroxidation products, and reduces the accumulation of them, PON1 prevents the acceleration of atherosclerosis, exhibits antioxidant ability, and assumes an antiatherogenic property. Negative correlation between the activity of PON1 and the level of lipid hydroperoxides in the rheumatoid arthritis patient group, overview
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additional information
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PON1 is a lipolactonase that associates with HDL-apolipoprotein A-I and thereby plays a role in the prevention of atherosclerosis
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additional information
?
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PON1 is a lipolactonase that associates with HDL-apolipoprotein A-I and thereby plays a role in the prevention of atherosclerosis
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additional information
?
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PON1 is not able to prevent macrophage oxidative stress, however, is able to retard macrophage-induced low-density lipoprotein oxidation
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additional information
?
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PON1 plays a key role in the protection of low density lipoproteins and high density lipoproteins from oxidation by hydrolyzing activated phospholipids and lipid peroxide products
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?
additional information
?
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PON1 plays a key role in the protection of low density lipoproteins and high density lipoproteins from oxidation by hydrolyzing activated phospholipids and lipid peroxide products
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?
additional information
?
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the active site of PON1 is characterized by two distinct binding regions, the hydrophobic binding site for arylesters/lactones and the paraoxon binding site for phosphotriesters
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?
additional information
?
-
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the active site of PON1 is characterized by two distinct binding regions, the hydrophobic binding site for arylesters/lactones and the paraoxon binding site for phosphotriesters
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?
additional information
?
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the enzyme carried by high-density lipoprotein, exerts a protective effect against oxidative damage of cells and lipoproteins, modulating the susceptibility of high-density lipoprotein to atherogenic modifications and has an anti-inflammatory role, low PON1 activity is a risk factor for cardiovascular disease
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?
additional information
?
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the enzyme is located on high-density lipoprotein and prevents low-density-lipoprotein and high-density-lipoprotein oxidation both in vivo and in vitro through hydrolysis of lipid peroxides, the risk of ischemic stroke is related to the genotype of PON1 192RQ polymorphisms, overview
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?
additional information
?
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no activity with chlorpyrifos oxon and diazoxon
-
-
?
additional information
?
-
no activity with chlorpyrifos oxon and diazoxon
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-
?
additional information
?
-
the overall substrate preference of cAMPP is methylphosphonates > ethylphosphonates >/= organophosphates
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-
?
additional information
?
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the enzyme does not hydrolyse 4-nitrophenyl phenyl ethylphosphonate and 1-methylpropyl 4-nitrophenyl ethylphosphonate
-
-
?
additional information
?
-
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recombinant human paraoxoanase 1 cannot hydrolyze dimethyl paraoxon even at higher concentrations used
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-
?
additional information
?
-
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the stereochemical preference of the wild-type enzyme with chemical warfare agents is for the RP enantiomers
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-
?
additional information
?
-
human paraoxonase 1 (h-PON1) can hydrolyze a variety of substrates. Interaction of recombinant PON1 proteins with lactones, overview
-
-
?
additional information
?
-
-
human paraoxonase 1 (h-PON1) can hydrolyze a variety of substrates. Interaction of recombinant PON1 proteins with lactones, overview
-
-
?
additional information
?
-
human PON1 is a calcium-dependent promiscuous enzyme (phosphotriesterase, arylesterase and lactonase) with a wide range of substrates. Human PON1 is capable of hydrolyzing a broad range of organophosphorus compounds, including paraoxon, diisopropylfluorophosphate (DFP) and nerve agents such as sarin, soman and VX
-
-
?
additional information
?
-
PON1 shows very good adaptability in assay development with different substrates, PON1 substrate exhibit many degrees of freedom in docking simulations. The relative chemiluminescent efficiency for the five acridinium esters ranks as the following descreasing order: unsubstituted, 2,4-dimethylphenoxy-, 4-methylphenoxy-, 4-tertbutylphenoxy-, and chloro-acridinium ester. Selfhydrolysis of the five acridinium esters in Tris-HCl buffer, at pH 7.5 and 25°C is insignificant during 22 min, but between 1.03% and 27.17% after 12 weeks
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-
?
additional information
?
-
-
the enzyme is also active with substrates of EC 3.1.1.81, quorum-quenching N-acyl-homoserine lactonase, and EC 3.1.8.2, diisopropyl-fluorophosphatase, hydrolyzing diisopropyl-fluorophosphates and phosphorus-halide and phosphorus-cyanide bonds in organophosphorus compounds. Measurement of N-oxodecanoyl-DL-homoserine lactone (3O-C10AHL)-hydrolyzing activity (EC 3.1.1.81) of recombinant h-PON1 enzymes is determined by using a recombinant quorum-sensing reporter Escherichia coli strain
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?
additional information
?
-
the enzyme is also active with substrates of EC 3.1.1.81, quorum-quenching N-acyl-homoserine lactonase, and EC 3.1.8.2, diisopropyl-fluorophosphatase, hydrolyzing diisopropyl-fluorophosphates and phosphorus-halide and phosphorus-cyanide bonds in organophosphorus compounds. Measurement of N-oxodecanoyl-DL-homoserine lactone (3O-C10AHL)-hydrolyzing activity (EC 3.1.1.81) of recombinant h-PON1 enzymes is determined by using a recombinant quorum-sensing reporter Escherichia coli strain
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?
additional information
?
-
the recombinant His6-tagged organophosphorus hydrolase (His6-OPH) shows catalytic activity in hydrolytic reactions with methylphosphonic acid (MPA) monoesters and diesters being decomposition products of R-VX nerve agent derivative (O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX), analysis of the mechanism of C-P bond cleavage in methylphosphonic acid by His6-OPH, overview. The recombinant enzyme His6-OPH is capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by chemical detoxification of R-VX, the RMs are multisubstrate mixtures for this enzyme, R-VX decomposition scheme. GC-MS analysis of phosphonates and methane
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-
?
additional information
?
-
-
the enzyme associated to high-density lipoproteins HDL exhibits antioxidant function of particular physiological relevance, mechanism, compositional fluctuations of HDL effects the influence of the enzyme on cardiovascular risks, overview
-
-
?
additional information
?
-
-
enzyme-HDL complex: interaction and structure analysis
-
-
?
additional information
?
-
molecular docking of substrates to wild-type and mutant enzymes using the crystal structure of PON1 (PDB ID 3SRG with resolution 2.19 A), overview. The enzyme also shows arylesterase activity (EC 3.1.1.2) with phenyl acetate as substrate
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?
additional information
?
-
th enzyme is also active with the chromogenic lactone thiobutyl-gamma-butyric lactone (TBBL), cf. EC 3.1.1.81. It has both lactonase activity and paraoxonase activity. Empirical valence bond simulations of PON1-catalyzed hydrolyses of paraoxon and TBBL, overview
-
-
?
additional information
?
-
the mammalian serum paraoxonase 1 (PON1) is a calcium-dependent serum esterase which catalyzes the hydrolysis of a broad range of organic esters and organophosphorous (OP) compounds. PON1 is a lactonase with native substrates gamma- and delta-lactones which have long alkyl side chains, and PON1 possesses promiscuous OP hydrolase activity, particularly on paraoxon, which is attributed to its considerable plasticity of catalytic structure
-
-
?
additional information
?
-
the mammalian serum paraoxonase 1 (PON1) is a calcium-dependent serum esterase which catalyzes the hydrolysis of a broad range of organic esters and organophosphorous (OP) compounds. PON1 is a lactonase with native substrates gamma- and delta-lactones which have long alkyl side chains, and PON1 possesses promiscuous OP hydrolase activity, particularly on paraoxon, which is attributed to its considerable plasticity of catalytic structure
-
-
?
additional information
?
-
-
improved degradation of organophosphorus pesticides by the engineered cell surface-expressed PON1 of transformeted strain JS444
-
-
?
additional information
?
-
-
improved degradation of organophosphorus pesticides by the engineered cell surface-expressed PON1 of transformeted strain JS444
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81
-
-
?
additional information
?
-
-
enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81
-
-
?
additional information
?
-
-
enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81
-
-
?
additional information
?
-
-
enzyme additionally displays L-homoserine lactonase activity, EC 3.1.1.81
-
-
?
additional information
?
-
-
a organophosphate-degrading enzyme
-
-
?
additional information
?
-
-
substrate specificity with organophosphorous compounds, overview
-
-
?
additional information
?
-
the enzyme possesses phosphotriesterase and a very high lactonase activity, structure-function relationship, overview
-
-
?
additional information
?
-
-
the enzyme possesses phosphotriesterase and a very high lactonase activity, structure-function relationship, overview
-
-
?
additional information
?
-
the phosphotriesterase-like lactonase enzyme is bifunctional showing lactonase (EC 3.1.1.81) and phosphotriesterase (EC 3.1.8.1 and 3.1.8.2) activities
-
-
?
additional information
?
-
substrate docking analysis. The C258 residue in the active site is involved in an interaction with the oxygen atom from the amide in the lactone analogue
-
-
?
additional information
?
-
the enzyme shows lactonase activity with N-(3-oxodecanoyl)-L-homoserine lactone, undecanoic-gamma-lactone, and undecanoic-delta-lactone, cf. EC 3.1.1.81
-
-
?
additional information
?
-
-
the enzyme shows lactonase activity with N-(3-oxodecanoyl)-L-homoserine lactone, undecanoic-gamma-lactone, and undecanoic-delta-lactone, cf. EC 3.1.1.81
-
-
?
additional information
?
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
a organophosphate-degrading enzyme
-
-
?
additional information
?
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
substrate specificity with organophosphorous compounds, overview
-
-
?
additional information
?
-
organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex
-
-
?
additional information
?
-
-
organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex
-
-
?
additional information
?
-
OPH is known to cleave the third ester linkage found in structurally diverse groups of organophosphorous compounds
-
-
?
additional information
?
-
-
OPH is known to cleave the third ester linkage found in structurally diverse groups of organophosphorous compounds
-
-
?
additional information
?
-
reactions are performed with soluble recombinant enzyme in solution or immobilized recombinant enzyme in calcium alginate gel
-
-
?
additional information
?
-
-
reactions are performed with soluble recombinant enzyme in solution or immobilized recombinant enzyme in calcium alginate gel
-
-
?
additional information
?
-
phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) possesses a rather broad substrate profile and is able to hydrolyze insecticides, plasticizers, and flame retardants that are not typically substrates for other enzymes of this class
-
-
?
additional information
?
-
enzyme substrate specificity analysis, optimization of the assay method, overview. Sb-PTE is capable of hydrolyzing a wide range of organophosphate triester substrates. Broensted analysis, P-O bond cleavage is rate limiting for kcat and kcat/Km for the dibutyl series of substrates tested. The enzyme must be able to effectively protonate the leaving group for efficient catalysis, but the lack of loss of activity at high pH is inconsistent with a separate general acid serving this role, unless it occurs after the rate-limiting step. The absence of a solvent viscosity effect and the magnitude of the 18O-isotope effects are consistent with nucleophilic attack of hydroxide as the rate-limiting step during the hydrolysis of substrates by this enzyme
-
-
?
additional information
?
-
multiple reaction products from the hydrolysis of chiral and prochiral organophosphate substrates, substrate specificity and differential hydrolysis of the prochiral substituents, analysis of the enantioselectivity, overview
-
-
?
additional information
?
-
no hydrolysis: ethyl parathion. The enzyme also shows activity of 1,4-lactonase (EC 3.1.1.25) and arylesterase (EC 3.1.1.2)
-
-
?
additional information
?
-
-
no hydrolysis: ethyl parathion. The enzyme also shows activity of 1,4-lactonase (EC 3.1.1.25) and arylesterase (EC 3.1.1.2)
-
-
?
