Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | heat-induced activation at 60°C with methyl paraoxon as substrate | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
from pLybAC11 vector overexpressed in Escherichia coli driven from the Escherichia coli rhamnose (rhaBAD) promoter. Plasmid bearing the GsP gene was transformed into Escherichia coli strain BW25113 | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
in the presence of Co2+, by vapor diffusion method in hanging drop, to 2.36 A resolution. The active site contains three substrate binding pockets: the small (F28, Y30, T70, C74, V268, and W271), large (R230, I233, M236, V237, and W289) and leaving group (Y100 and E103) pockets. Metal binding site contains a tyrosine residue at position 97 in close proximity to the beta-metal | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
methyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
2.1 | - |
ethyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
6.1 | - |
demeton-S | at 35°C, pH 7.2 | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity is highest with Co2+ present in the active site | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus 10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
in the presence of Co2+, by centrifugation and on Co2+ affinity resin, to over 95% | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, 1 month | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
demeton-S + H2O | - |
Geobacillus stearothermophilus | 2-ethylsulfanyl-ethanethiol + diethyl phosphate | - |
? | |
demeton-S + H2O | - |
Geobacillus stearothermophilus 10 | 2-ethylsulfanyl-ethanethiol + diethyl phosphate | - |
? | |
ethyl paraoxon + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + diethyl phosphate | - |
? | |
ethyl paraoxon + H2O | - |
Geobacillus stearothermophilus 10 | 4-nitrophenol + diethyl phosphate | - |
? | |
methyl paraoxon + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + dimethyl phosphate | - |
? | |
methyl paraoxon + H2O | - |
Geobacillus stearothermophilus 10 | 4-nitrophenol + dimethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | with methyl paraoxon as a substrate, retains full activity after incubation for 15 min between 25-90°C. Can withstand an incubation temperature of 60°C for two days, and retains 72% of its activity after incubation at 90°C for 2 h. The melting temperature is 106.6°C | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
demeton-S | at 35°C, pH 7.2 | Geobacillus stearothermophilus | |
0.00783 | - |
methyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
0.115 | - |
ethyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000983 | - |
demeton-S | at 35°C, pH 7.2 | Geobacillus stearothermophilus | |
0.029 | - |
methyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
0.05467 | - |
ethyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus |