Literature summary for 3.1.8.1 extracted from

Blum, M.M.; Loehr, F.; Richardt, A.; Rueterjans, H.; Chen, J.C.
Binding of a designed substrate analogue to diisopropyl fluorophosphatase: implications for the phosphotriesterase mechanism (2006), J. Am. Chem. Soc., 128, 12750-12757.

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Protein Variants

Protein Variants	Comment	Organism
D229N/N120D	site-directed mutagenesis, the mutation reorientates the electrostatic environment around the catalytic calcium, inactive mutant	Loligo vulgaris
D229N/N175D	site-directed mutagenesis, the mutation reorientates the electrostatic environment around the catalytic calcium, inactive mutant	Loligo vulgaris
E21Q/N120D	site-directed mutagenesis, inactive mutant	Loligo vulgaris
E21Q/N175D	site-directed mutagenesis, inactive mutant	Loligo vulgaris

Inhibitors

Inhibitors	Comment	Organism	Structure
O,O-dicyclopentylphosphoroamidate	substrate analogue, competitive inhibition, binding structure, direct coordination of the phosphoryl oxygen by the active site catalytic calcium ion, overview	Loligo vulgaris

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required for catalysis, bound at the active site, coordinating residues are E21 and D229	Loligo vulgaris

Organism

Organism	UniProt	Comment	Textmining
Loligo vulgaris	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol	catalytic mechanism	Loligo vulgaris	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sarin + H2O	a highly toxic organophosphorus nerve agent	Loligo vulgaris	methylphosphonofluoride acid + ?	-	?
soman + H2O	i.e. pinacolyl methylphosphonofluoridate, a highly toxic organophosphorus nerve agent	Loligo vulgaris	methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol	-	?
tabun + H2O	a highly toxic organophosphorus nerve agent	Loligo vulgaris	?	-	?

Synonyms

Synonyms	Comment	Organism
DFPase	-	Loligo vulgaris
diisopropyl fluorophosphatase	-	Loligo vulgaris
phosphotriesterase	-	Loligo vulgaris

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Release 2023.1 (January 2023)