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Literature summary for 3.1.8.1 extracted from

  • Yeung, D.T.; Lenz, D.E.; Cerasoli, D.M.
    Analysis of active-site amino-acid residues of human serum paraoxonase using competitive substrates (2005), FEBS J., 272, 2225-2230.
    View publication on PubMed
Search for more literature for 3.1.8.1

Protein Variants

Protein Variants Comment Organism
F222Y hydrolysis of phenyl acetate, but not of paraoxon Homo sapiens
H115W no hydrolysis of phenyl acetate, but hydrolysis of paraoxon. Phenyl acetate can act as an inhibitor Homo sapiens
H115W/N133S no hydrolysis of phenyl acetate, but hydrolysis of paraoxon Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
 Homo sapiens
phenyl acetate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 paraoxon pH 10.5, 25°C, mutant enzyme H115W/N133S Homo sapiens
0.25
-
 paraoxon pH 10.5, 25°C, wild-type enzyme Homo sapiens
0.31
-
 paraoxon pH 8.5, 25°C, wild-type enzyme Homo sapiens
0.43
-
 paraoxon pH 10.5, 25°C, mutant enzyme H115W Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PON1
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.44
-
 diisopropylfluorophosphate pH 8.5, 25°C, wild-type enzyme, substrate paraoxon Homo sapiens
0.47
-
 phenyl acetate pH 8.5, 25°C, wild-type enzyme, substrate paraoxon Homo sapiens
0.91
-
 phenyl acetate pH 10.5, 25°C, wild-type enzyme, substrate paraoxon Homo sapiens
1.95
-
 phenyl acetate pH 10.5, 25°C, mutant enzyme H115W, substrate paraoxon Homo sapiens