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3.1.8.1: aryldialkylphosphatase

This is an abbreviated version!
For detailed information about aryldialkylphosphatase, go to the full flat file.

Word Map on EC 3.1.8.1

Reaction

An aryl dialkyl phosphate
+
H2O
=
dialkyl phosphate
 +
an aryl alcohol

Synonyms

A-esterase, A7Q26_23485, aminopeptidase P, AMPP, aryldialkylphosphatase, arylesterase, aryltriphosphatase, bacterial phosphotriesterase, DFPase, diisopropyl fluorophosphatase, esterase B1, esterase E4, esterase, organophosphate, esterase, paraoxon, esterase, pirimiphos-methyloxon, G3C9 rePON1, h-PON1, HAD, haloalkylphosphorus hydrolase, HDL-associated esterase/lactonase paraoxonase 1, HDL-PON1, high activity paraoxonase, high-density lipoprotein-associated esterase/lactonase, human paraoxonase 1, HuPON1, inner membrane protein YiaH, lactonase SsoPox, lactonase/phosphotriesterase, low activity paraoxonase, methyl parathion hydrolase, More, Mph, mPHP, OP hydrolase, OP-hydrolase, OP-hydrolyzing enzyme, OPA anhydrase, opd, OpdA, OpdD, OPH, OPHC2, organophosphate hydrolase, organophosphorous hydrolase, organophosphorus acid anhydrase, organophosphorus hydrolase, organophosphorus pesticide hydrolase, organophosphorus-hydrolyzing enzyme, paraoxon hydrolase, paraoxonase, paraoxonase 1, paraoxonase 1A, paraoxonase 3, paraoxonase-1, paraoxonase-2, paraoxonase1, parathion hydrolase, phosphotriesterase, phosphotriesterase homology protein, phosphotriesterase-like lactonase, PHP, pirimiphos-methyloxon esterase, PLL, PO.ase, PON, PON 1, PON-1, PON-aryl, PON-para, PON1, PON1A, PON2, PON3, POX, PTE, PTE S5, Rv0230c, SACI2140, Saci_2140, SacPox, Sb-PTE, serum paraoxonase, serum paraoxonase 1, SisLac, SisPox, Sso, SSO2522, SsoPox, type A paraoxonase, type B paraoxonase, VmoLac, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.1 aryldialkylphosphatase

Specific Activity

Specific Activity on EC 3.1.8.1 - aryldialkylphosphatase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000008
-
wild type enzyme, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00015
-
wild type enzyme, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00019
-
mutant enzyme A80V/F132C/K185R/D208G/H257W/I274N/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00022
-
mutant enzyme A80V/K185R/D208G/H257W/I274N/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00042
-
purified recombinant enzyme, substrate paraoxon
0.00045
-
mutant enzyme A80V/F132D/K185R/D208G/H257W/I274N/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00048
-
mutant enzyme A80V/K185R/D208G/I274N/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00075
-
mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.0012
wild type enzyme from crude extract, using paraoxon as substrate
0.0013
mutant enzyme Q192R from crude extract, using paraoxon as substrate
0.00151
-
mutant enzyme A80V/K185R/D208G/I274N/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.0022
-
purified recombinant enzyme, substrate methyl paraoxon
0.00414
-
mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00429
-
mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/F306V/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00472
-
mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00509
-
mutant enzyme A80V/K185R/D208G/H257W/I274N/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.00617
-
mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.007
-
mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, using VX as substrate, in 10 mM N-Tris (hydroxymethyl-3-amino propanesulfonic acid), pH 8.0 , at 25°C
0.00711
-
crude enzyme, at pH 8.0, 37°C
0.01159
-
mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.01404
-
mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.01511
-
mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/F306V/R319S, using malathion as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.0194
-
mutant enzyme A80V/F132D/K185R/D208G/H257W/I274N/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.02677
-
mutant enzyme A80V/F132C/K185R/D208G/H257W/I274N/R319S, using demeton-S methyl as substrate, in 50 mM HEPES buffer, pH 7.5, at pH, 25°C
0.0803
-
mutant enzyme A80V/I106V/F132D/K185R/D208G/H257W/I274N/R319S, using VX as substrate, in 10 mM N-Tris (hydroxymethyl-3-amino propanesulfonic acid), pH 8.0 , at 25°C
0.083
substrate O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, pH 8.0, 25°C, enzyme mutant C23 administered in guinea pigs in vivo
0.097
-
wild type enzyme, using VX as substrate, in 10 mM N-Tris (hydroxymethyl-3-amino propanesulfonic acid), pH 8.0 , at 25°C
0.1
about, wild-type enzyme, substrate chlorpyrifos
0.15
purified recombinant PON1 wild-type enzyme, pH 10.5, 25°C, substrate diethyl-paraoxon
0.18
purified recombinant PON1-hFc fusion enzyme, pH 10.5, 25°C, substrate diethyl-paraoxon
0.205
pH 8.5, 25°C, substrate: paraoxon, purified recombinant enzyme
0.344
-
crude enzyme, pH 8.0, 37°C
0.391
-
-
0.42
purified recombinant enzyme mutant W263F, pH 8.5, 65°C, substrate diethyl-paraoxon
0.567
substrate cyclosarin, pH 8.0, 25°C, enzyme administered in guinea pigs in vivo
0.611
-
using paraoxin as substrate (paraoxonase activity of PON1), at 25°C
0.85
-
plasma, at 37°C
0.94
mutant enzyme Q192R after 1018fold purification, using paraoxon as substrate
0.962
-
purified enzyme from phenotype A blood plasma
1
about, genetic variants A1030 and A1467, substrate chlorpyrifos
1.21
wild type enzyme after 1018fold purification, using paraoxon as substrate
1.4
substrate cyclosarin, pH 8.0, 37°C, enzyme administered in guinea pigs in vivo
1.76
-
Q192 isoenzyme, serum, pH 8 at 37°C, in Tris-base buffer
1.88
-
isozyme 192Q, crude enzyme, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C
1.94
pH 8.5, 25°C, substrate: paraoxon, purified recombinant enzyme
10.78
-
enzyme of breed Swiss Black, pH 8.0, 25°C
113
pH 8.5, 25°C, substrate: diazoxon, purified recombinant enzyme
1168
-
isozyme 192R, crude enzyme, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C
12.03
-
substrate paraoxon, wild-type enzyme, pH and temperature not specified in the publication
12.3
-
substrate: paraoxon, pH 8.5, 70°C, Mn2+-containing enzyme
12.8
purified recombinant enzyme mutant W263F, pH 8.5, 65°C, substrate diethyl-paraoxon
12.84
-
after 37.3fold purification, pH 8.0, 37°C
122.6
substrate paraoxon, enzyme mutant C258L/I261F/W263A, pH and temperature not specified in the publication
123.6
purified recombinant enzyme mutant C258L/I261F/W263A, pH 8.5, 65°C, substrate diethyl-paraoxon
1370
-
37°C, pH 8.5
14.92
-
after 1690fold purification, at pH 8.0, 37°C
168
recombinant enzyme in presence of Co2+
1730
-
purified serum enzyme
2.5
-
mutant enzyme G60V/A80V/I106V/F132D/K185R/D208G/H257W/I274N/F306V/R319S, using VX as substrate, in 10 mM N-Tris (hydroxymethyl-3-amino propanesulfonic acid), pH 8.0 , at 25°C
2109
-
Q192 isoenzyme, 901fold purified, pH 8 at 37°C, in Tris-base buffer
22.4
-
enzyme of breed Montofon, pH 8.0, 25°C
227.3
-
pH 8.0, 37°C
288
-
pH 8.0, 37°C
3221
-
isozyme 192R, after 742fold purification, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C
33
-
substrate ethyl parathion
3333
-
R192 isoenzyme, 453fold purified, pH 8 at 37°C, in Tris-base buffer
34
recombinant enzyme in absence of Co2+
35
about, genetic variant B3561, substrate chlorpyrifos
3840
-
in 50 mM glycine/NaOH, pH 10.5, 1 mM CaCl2, at 25°C
4.34
-
isozyme 192Q, after 589.7fold purification, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C
40.9
pH 8.5, 25°C, substrate: chlorpyrifos oxon, purified recombinant enzyme
438.2
-
515fold purified enzyme, at 37°C
465
recombinant enzyme after 90fold purification
5
about, genetic variant B2136, substrate chlorpyrifos
5.15
recombinant enzyme from crude cell extract
5.21
-
R192 isoenzyme, serum, pH 8 at 37°C, in Tris-base buffer
53.8
purified recombinant His-tagged mature enzyme
6.64
-
purified enzyme from phenotype A blood plasma
76.3
-
purified enzyme, substrate 4-nitrophenyl phosphate at pH 7.0 and 25°C
8310
-
substrate methyl paraoxon, 30°C, pH 8.0
additional information