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Literature summary for 3.1.8.1 extracted from

  • Mulbry, W.
    Characterization of a novel organophosphorus hydrolase from Nocardiodes simplex NRRL B-24074 (2000), Microbiol. Res., 154, 285-288.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
ethyl parathion
-
Pimelobacter simplex
0.345
-
ethyl phenyl parathion
-
Pimelobacter simplex

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pimelobacter simplex 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Pimelobacter simplex

Organism

Organism UniProt Comment Textmining
Pimelobacter simplex
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
activity lost during incubation in phosphate buffer can be restored by addition of CoSO4 or CuSO4 Pimelobacter simplex

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33
-
substrate ethyl parathion Pimelobacter simplex

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl parathion + H2O
-
Pimelobacter simplex ?
-
?
ethyl phenyl parathion
-
Pimelobacter simplex ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Pimelobacter simplex