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Literature summary for 3.1.8.1 extracted from

  • Bayrak, A.; Bayrak, T.; Demirpence, E.; Kilinc, K.
    Differential hydrolysis of homocysteine thiolactone by purified human serum (192)Q and (192)R PON1 isoenzymes (2011), J. Chromatogr. B, 879, 49-55.
    View publication on PubMed
Search for more literature for 3.1.8.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 paraoxon isozyme 192R, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
0.96
-
 paraoxon isozyme 192Q, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.88
-
 isozyme 192Q, crude enzyme, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
4.34
-
 isozyme 192Q, after 589.7fold purification, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
1168
-
 isozyme 192R, crude enzyme, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3221
-
 isozyme 192R, after 742fold purification, using paraoxon as substrate, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O i.e. O,O'-diethyl-4-nitrophenyl phosphothioate Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11
-
 paraoxon isozyme 192Q, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens
3.67
-
 paraoxon isozyme 192R, in 100 mM Tris-HCl (pH 8.5), 1 mM CaCl2, 37°C Homo sapiens