Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.8.1: aryldialkylphosphatase

This is an abbreviated version!
For detailed information about aryldialkylphosphatase, go to the full flat file.

Word Map on EC 3.1.8.1

Reaction

An aryl dialkyl phosphate
+
H2O
=
dialkyl phosphate
 +
an aryl alcohol

Synonyms

A-esterase, A7Q26_23485, aminopeptidase P, AMPP, aryldialkylphosphatase, arylesterase, aryltriphosphatase, bacterial phosphotriesterase, DFPase, diisopropyl fluorophosphatase, esterase B1, esterase E4, esterase, organophosphate, esterase, paraoxon, esterase, pirimiphos-methyloxon, G3C9 rePON1, h-PON1, HAD, haloalkylphosphorus hydrolase, HDL-associated esterase/lactonase paraoxonase 1, HDL-PON1, high activity paraoxonase, high-density lipoprotein-associated esterase/lactonase, human paraoxonase 1, HuPON1, inner membrane protein YiaH, lactonase SsoPox, lactonase/phosphotriesterase, low activity paraoxonase, methyl parathion hydrolase, More, Mph, mPHP, OP hydrolase, OP-hydrolase, OP-hydrolyzing enzyme, OPA anhydrase, opd, OpdA, OpdD, OPH, OPHC2, organophosphate hydrolase, organophosphorous hydrolase, organophosphorus acid anhydrase, organophosphorus hydrolase, organophosphorus pesticide hydrolase, organophosphorus-hydrolyzing enzyme, paraoxon hydrolase, paraoxonase, paraoxonase 1, paraoxonase 1A, paraoxonase 3, paraoxonase-1, paraoxonase-2, paraoxonase1, parathion hydrolase, phosphotriesterase, phosphotriesterase homology protein, phosphotriesterase-like lactonase, PHP, pirimiphos-methyloxon esterase, PLL, PO.ase, PON, PON 1, PON-1, PON-aryl, PON-para, PON1, PON1A, PON2, PON3, POX, PTE, PTE S5, Rv0230c, SACI2140, Saci_2140, SacPox, Sb-PTE, serum paraoxonase, serum paraoxonase 1, SisLac, SisPox, Sso, SSO2522, SsoPox, type A paraoxonase, type B paraoxonase, VmoLac, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.1 aryldialkylphosphatase

Temperature Stability

Temperature Stability on EC 3.1.8.1 - aryldialkylphosphatase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
purified recombinant enzyme, half-life is 90 min
102
Tm-value, wild-type enzyme
106
estimated denaturation temperature
20
-
plasma enzyme stable, liver enzyme less stable
25 - 90
-
with methyl paraoxon as a substrate, retains full activity after incubation for 15 min between 25-90°C. Can withstand an incubation temperature of 60°C for two days, and retains 72% of its activity after incubation at 90°C for 2 h. The melting temperature is 106.6°C
27
-
24 h, 80% loss of activity of rat liver and plasma enzyme
30 - 60
the thermostability of hydrolyric activity of recombinant PON1-hFc is slightly increased compared to recombinant wild-type PON1, inactivation of PON1 at 55°C, inactivation of PON1-hFc at 60°C, profiles overview
40
-
3 min, purified enzyme, stable up to
40 - 55
180 min, quite stable
45
-
60 min, stable
47.5
-
30 min, 50% loss of activity
50 - 94
the enzyme retains 52% of its activity after 50 h of incubation at 90°C, the enzyme is rapidly inactivated above 94°C, most of the enzyme activity is maintained for 5 days at 50°C, the enzyme activity gradually decreases with time at higher temperatures, approximately 70% of the enzyme activity remains after 5 days at 70°C, and 52% of it is still preserved after 50 h at 90°C, however, at 90°C after 5 days, the enzyme is completely inactivated
52
-
inactivation
61
-
the melting point of the native enzyme is at 61°C
70 - 85
-
purified recombinant enzyme, 4 h, completely stable
73
-
the melting point of the recombinant enzyme is at 73°C
94
Tm-value, mutant enzyme Q34Q
96
Tm-value, mutant enzyme E14K
98
Tm-value, mutant enzyme E14K/Y34Q
additional information