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3.1.8.1: aryldialkylphosphatase

This is an abbreviated version!
For detailed information about aryldialkylphosphatase, go to the full flat file.

Word Map on EC 3.1.8.1

Reaction

An aryl dialkyl phosphate
+
H2O
=
dialkyl phosphate
 +
an aryl alcohol

Synonyms

A-esterase, A7Q26_23485, aminopeptidase P, AMPP, aryldialkylphosphatase, arylesterase, aryltriphosphatase, bacterial phosphotriesterase, DFPase, diisopropyl fluorophosphatase, esterase B1, esterase E4, esterase, organophosphate, esterase, paraoxon, esterase, pirimiphos-methyloxon, G3C9 rePON1, h-PON1, HAD, haloalkylphosphorus hydrolase, HDL-associated esterase/lactonase paraoxonase 1, HDL-PON1, high activity paraoxonase, high-density lipoprotein-associated esterase/lactonase, human paraoxonase 1, HuPON1, inner membrane protein YiaH, lactonase SsoPox, lactonase/phosphotriesterase, low activity paraoxonase, methyl parathion hydrolase, More, Mph, mPHP, OP hydrolase, OP-hydrolase, OP-hydrolyzing enzyme, OPA anhydrase, opd, OpdA, OpdD, OPH, OPHC2, organophosphate hydrolase, organophosphorous hydrolase, organophosphorus acid anhydrase, organophosphorus hydrolase, organophosphorus pesticide hydrolase, organophosphorus-hydrolyzing enzyme, paraoxon hydrolase, paraoxonase, paraoxonase 1, paraoxonase 1A, paraoxonase 3, paraoxonase-1, paraoxonase-2, paraoxonase1, parathion hydrolase, phosphotriesterase, phosphotriesterase homology protein, phosphotriesterase-like lactonase, PHP, pirimiphos-methyloxon esterase, PLL, PO.ase, PON, PON 1, PON-1, PON-aryl, PON-para, PON1, PON1A, PON2, PON3, POX, PTE, PTE S5, Rv0230c, SACI2140, Saci_2140, SacPox, Sb-PTE, serum paraoxonase, serum paraoxonase 1, SisLac, SisPox, Sso, SSO2522, SsoPox, type A paraoxonase, type B paraoxonase, VmoLac, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.1 aryldialkylphosphatase

Cloned

Cloned on EC 3.1.8.1 - aryldialkylphosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a high level of recombinant hPON1 type A (rPON1A) was produced by Hi-5 insect cells. A fraction is secreted into the cell culture medium, but the majority remains associated with the host insect cells
-
application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis
bacterially expressed chimeric G2E6
-
directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state
distribution of three PON1 polymorphisms in individuals of different age, overview
-
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain HB101 and BL21(DE3)
-
expressed from Trichoplusia ni larvae
-
expressed in baculovirus system in Hi5 insect cells
expressed in Drosophila melanogaster
expressed in Escherichia coli
expressed in Escherichia coli BL-21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli DH5 alpha cells
expressed in Escherichia coli strain SG13009
-
expressed in Synechococcus PCC7942 strain R2-SPc
expressed in Trichoplusia ni larvae
-
expression in Escherichia coli
expression of His-tagged wild-type and mutants
-
expression of His6-tagged OPH in enzyme-deficient Escherichia coli strain SG13009
-
expression of His6-tagged OPH in Escherichia coli strains DH5alpha and SG13009, the His6-tagged enzyme shows altered properties compared to the wild-type enzyme
-
expression of mutant enzyme
expression of N-terminally His6-tagged wild-type and 3-fluorotyrosine-containing OPH in Escherichia coli strain B-2935 devoid of the metabolic ways of tyrosine biosynthesis, the presence of 3-F-Tyr in the medium inhibits the cell growth
-
expression of PON1 genetic variant G2E6 as thioredoxin-fusion protein, fusion via a His6-linker, in Escherichia coli
expression of PON1 mutant enzymes in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli strain BL21
expression of wild-type PON1 and PON1-human phosphate-binding protein hybrid in Escherichia coli
-
from pLybAC11 vector overexpressed in Escherichia coli driven from the Escherichia coli rhamnose (rhaBAD) promoter. Plasmid bearing the GsP gene was transformed into Escherichia coli strain BW25113
-
functional expression of mpd and pytH in Pseudomonas putida KT2440 under the control of a constitutive BioBrick promoter J23119
gene is synthesized with an N-terminal linker containing a Strep-tag and a TEV cleavage site in Escherichia coli
gene mpd, subcloning in Escherichia coli strain DH5alpha, expression of functional His-tagged enzyme in Escherichia coli strain BL21(DE3) improved by elimination of the signal peptide
gene opd is encoded by the organophosphate degradation (opd) island, recombinant expression of His10-tagged wild-type enzyme in Brevundimonas diminuta enzyme-deficient strain DS010. Protein-protein interactions study using the bacterial two-hybrid system in Escherichia coli strain BTH101
gene opd, expression of His-tagged wild-type and mutant enzymes
-
gene opd, expression of the gene with and without secretory leader sequence using the tac and taclac promoters
-
gene opd, plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis is modified to be transferred back to this bacterium. The replication function of Sphingobium amiense plasmid is inserted at downstream of OPH gene, and Shingobium fuliginis is transformed with this plasmid, pTV118N-His-OPH-ORF4, the transformant is designated KGU0379. The transformant produces larger amount of active His-tagged OPH than Escherichia coli. Recombinant expression of plasmid pTV118N-His-OPH-ORF4 also in Sphingomonas paucimobilis strain KGU0001 (originally IAM 12576), the transformant is designated KGU0400. Recombinant expression in Escherichia coli resulting in strain KGU0255
gene opd, recombinant expression of wild-type and mutant enzymes, coexpression of CAW-tagged OPH and N-terminally His6-tagged TonB
gene opd, recombinant expression of wild-type and mutant MBP-tagged enzymes in Escherichia coli strain BL21(DE3)
gene opdA, expression in Escherichia coli strain DH5alpha, growth in media supplemented with different divalent metal ions, overview
gene opdD, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3)
-
gene PON1 genotyping, analysis of the relationship between genotype, prenatal exposure to organophosphate insecticides, and autism spectrum disorders and related behaviors, overview
gene PON1, DNA and amino acid sequence determination and analysis, gene structure, expression in CHO cells and in human hepatocyte Huh-7 cells
-
gene PON1, expression of two naturally occurring allelic variant isozymes L55R192 PON1 and L55Q192 PON1 in Escherichia coli strain DH5alpha and in CHO cells
-
gene PON1, phylogenetic analysis
gene PON1, recombinant expression of His6-tagged enzyme in Escherichia coli strain SG13009[pREP4]
gene PON1, recombinant expression of the human paraoxonase 1 in Drosophila melanogaster S2 stable cell line with induction by CuSO4 for 14 days. The recombinant human PON1 is fused with the human immunoglobulin Fc domain (PON1-hFc) to improve protein stability and purification efficiency, method optimization, overview. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) harboring the pGTf2 plasmid expressing GroEL-GroES and TF chaperones
gene PON1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies, subcloning in Escherichia coli strain DH5alpha
gene PON1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain Origami B (DE3)
gene PON1, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), mutants H115W/R192H and H115W/R192M are expressed as truncated proteins, subcloning in Escherichia coli strain DH5alpha
gene ssopox and gene ssopox-pte, sequence comparisons, recombinant expression of wild-type, point mutation, and chimeric mutant enzymes in Escherichia coli strains TOP10 and BL21(DE3)
genotyping of naturally occuring polymorphisms, allele frequency determination
genotyping of PON1_192 and PON1_55
-
heterologously expressed in Escherichia coli
ORF ophc2, subcloning in Escherichia coli strain JM109, functional high level expression of OPHC2 in Pichia pastoris strain GS115, secretion to the culture supernatant
overexpressed in Escherichia coli
overexpressed in Pseudomonas putida KT2440
overexpression in HeLa cells, expression as GST-fusion protein in Escherichia coli BL21
-
plasmid library screening, recombinant wild-type and mutant enzymes from Escherichia coli BL21(DE3)-pGro7/GroEL (TaKaRa) chaperone expressing strain by ammonium sulfate fractionation, desalting gel filtration, and gel filtration
PTEm gene without the 29-amino acid leader cloned into pET17b vector, expressed in Escherichia coli HMS 174 (DE3) pLysS. PCR fragments of mutants cloned into the expression plasmid pET17b and expressed in Escherichia coli BL21 (DE3) pLysS
recombinant expression of His-tagged mutant G137D in Escherichia coli strain BL21(DE3)
-
recombinant expression of mutant enzyme SsoPox 3 M in Escherichia coli strain BL21(DE3), induction by galactose, method optimization
recombinant expression of N-terminally His6-tagged enzyme
recombinant expression of the enzyme in Escherichia coli BL21(DE3)pLysS, subcloning in Escherichia coli strain DH5alpha. To achieve the extracellular secretion of enzyme OPH in Escherichia coli, the complete opd gene along with its native signal peptide is codon-optimized and expressed in Escherichia coli BL21(DE3)pLysS. The secretion of OPH into the extracellular medium is highly affected by culture conditions, e.g. Co2+ decreases the secretion of the enzyme, while IPTG causes an enhancement in extracellular OPH secretion
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type enzyme in Escherichia coli strain BL21(DE3), induction by galactose, method optimization
-
recombinant overexpression of the C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies
SacPox is expressed in the presence of 0.2 mM of each of the following salts: CdCl2, CoCl2, MnCl2, ZnCl2, NiSO4. The gene sacpox is cloned in the expression vector pT7-7, and overexpressed in Escherichia coli BL21(DE3) cells. The highest activity is obtained for the crude extract of a Mn2+-enriched culture
-
the cytosolic protein, with N-terminal hexahistidine tag, is expressed using the Escherichia coli Rosetta (DE3) strain
the recombinant enzyme is expressed in Trichoplusia ni High Five insect cells
wild-type and variants of gene opd, DNA and amino acid sequence determination of gene variants, overview, expression of OPHs at the cell surface of strain XL1-Blue using plasmid pINCOP