Any feedback?
Literature summary for 3.1.8.1 extracted from
- Evaluation of organophosphorus chemicals-degrading enzymes: a comparison of Escherichia coli and human cytosolic aminopeptidase P (2008), Chem. Biodivers., 5, 1401-1411.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the cytosolic protein, with N-terminal hexahistidine tag, is expressed using the Escherichia coli Rosetta (DE3) strain | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R153A | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
| R153L | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
| R153L/R370L | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
| R153W | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
| R153W/R370L | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
| R370L | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
| W88L | the mutant shows an increase of activity towards methyl 1-methylethyl 4-nitrophenyl phosphate and methyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme, the mutant shows a decrease of activity towards ethyl 4-nitrophenyl ethylphosphonate, 4-nitrophenyl propyl ethylphosphonate, and 1-methylethyl 4-nitrophenyl ethylphosphonate compared to the wild type enzyme | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Escherichia coli |  |
| Mn2+ | the enzyme is activated with 0.025 mM Mn2+ at 37°C for 1 h | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the overall substrate preference of cAMPP is methylphosphonates > ethylphosphonates >/= organophosphates | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P15034 | - |
- |
| Homo sapiens | Q9NQW7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap affinity column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-methylethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O | low activity | Homo sapiens | 4-nitrophenol + 1-methylethyl hydrogen (1-methylpropyl)phosphonate | - |
? | |
| 1-methylethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O | 6.8% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + 1-methylethyl hydrogen (1-methylpropyl)phosphonate | - |
? | |
| 1-methylethyl 4-nitrophenyl methylphosphonate + H2O | - |
Homo sapiens | 4-nitrophenol + 1-methylethyl hydrogen methylphosphonate | - |
? | |
| 1-methylethyl 4-nitrophenyl methylphosphonate + H2O | 33.1% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + 1-methylethyl hydrogen methylphosphonate | - |
? | |
| 1-methylpropyl 4-nitrophenyl methylphosphonate + H2O | best substrate | Homo sapiens | 4-nitrophenol + 1-methylpropyl hydrogen methylphosphonate | - |
? | |
| 1-methylpropyl 4-nitrophenyl methylphosphonate + H2O | 29.5% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + 1-methylpropyl hydrogen methylphosphonate | - |
? | |
| 4-nitrophenyl phenyl methylphosphonate + H2O | - |
Homo sapiens | 4-nitrophenol + phenyl hydrogen methylphosphonate | - |
? | |
| 4-nitrophenyl phenyl methylphosphonate + H2O | 45.2% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + phenyl hydrogen methylphosphonate | - |
? | |
| 4-nitrophenyl propyl methylphosphonate + H2O | best substrate | Homo sapiens | 4-nitrophenol + propyl hydrogen methylphosphonate | - |
? | |
| 4-nitrophenyl propyl methylphosphonate + H2O | 58.5% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + propyl hydrogen methylphosphonate | - |
? | |
| diethyl 4-nitrophenyl phosphate + H2O | low activity | Homo sapiens | 4-nitrophenol + diethyl hydrogen phosphate | - |
? | |
| diethyl 4-nitrophenyl phosphate + H2O | 15.1% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + diethyl hydrogen phosphate | - |
? | |
| dimethyl 4-nitrophenyl phosphate + H2O | 13.3% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + dimethyl hydrogen phosphate | - |
? | |
| ethyl 1-methylethyl 4-nitrophenyl phosphate + H2O | low activity | Homo sapiens | 4-nitrophenol + ethyl 1-methylethyl hydrogen phosphate | - |
? | |
| ethyl 1-methylethyl 4-nitrophenyl phosphate + H2O | 18.8% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + ethyl 1-methylethyl hydrogen phosphate | - |
? | |
| ethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O | - |
Homo sapiens | 4-nitrophenol + ethyl hydrogen (1-methylpropyl)phosphonate | - |
? | |
| ethyl 4-nitrophenyl (1-methylpropyl)phosphonate + H2O | 14.7% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + ethyl hydrogen (1-methylpropyl)phosphonate | - |
? | |
| ethyl 4-nitrophenyl methylphosphonate + H2O | low activity | Homo sapiens | 4-nitrophenol + ethyl hydrogen methylphosphonate | - |
? | |
| ethyl 4-nitrophenyl methylphosphonate + H2O | 29.8% relative specific activity compared to methyl 1-methylethyl 4-nitrophenyl phosphate | Escherichia coli | 4-nitrophenol + ethyl hydrogen methylphosphonate | - |
? | |
| methyl 1-methylethyl 4-nitrophenyl phosphate + H2O | 100% activity | Escherichia coli | 4-nitrophenol + methyl 1-methylethyl hydrogen phosphate | - |
? | |
| additional information | the overall substrate preference of cAMPP is methylphosphonates > ethylphosphonates >/= organophosphates | Homo sapiens | ? | - |
? | |
| additional information | the enzyme does not hydrolyse 4-nitrophenyl phenyl ethylphosphonate and 1-methylpropyl 4-nitrophenyl ethylphosphonate | Homo sapiens | ? | - |
? | |
| additional information | the enzyme does not hydrolyse 4-nitrophenyl phenyl ethylphosphonate and 1-methylpropyl 4-nitrophenyl ethylphosphonate | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase P | - |
Escherichia coli |
| AMPP | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | using CHES buffer | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
