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Literature summary for 3.1.8.1 extracted from

  • Trovaslet-Leroy, M.; Musilova, L.; Renault, F.; Brazzolotto, X.; Misik, J.; Novotny, L.; Froment, M.T.; Gillon, E.; Loiodice, M.; Verdier, L.; Masson, P.; Rochu, D.; Jun, D.; Nachon, F.
    Organophosphate hydrolases as catalytic bioscavengers of organophosphorus nerve agents (2011), Toxicol. Lett., 206, 14-23.
    View publication on PubMed

Application

Application Comment Organism
medicine human paraoxonase 1 is a catalytic bioscavenger for pre-treatment and therapy of organophosphorus compounds poisoning Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

General Stability

General Stability Organism
human paraoxonase 1 alone is unstable. But it is stabilized by the presence of its most common partner protein, the human phosphate binding protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
paraoxon native enzyme, in 50 mM borate buffer (pH 8.5, 0.1 mM CoCl2), at 25°C Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens
-
Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CVX + H2O i.e. diethylamino-ethyl-O-butyl methylphosphonothioate Homo sapiens ?
-
?
CVX + H2O i.e. diethylamino-ethyl-O-butyl methylphosphonothioate Brevundimonas diminuta ?
-
?
paraoxon + H2O
-
Homo sapiens 4-nitrophenol + diethyl phosphate
-
?
paraoxon + H2O
-
Brevundimonas diminuta 4-nitrophenol + diethyl phosphate
-
?
VR + H2O i.e. diethylamino-ethyl-O-isobutyl methylphosphonothioate Homo sapiens ?
-
?
VR + H2O i.e. diethylamino-ethyl-O-isobutyl methylphosphonothioate Brevundimonas diminuta ?
-
?
VX + H2O i.e. diisopropylamino-ethyl-O-ethyl methylphosphonothioate Homo sapiens ?
-
?
VX + H2O i.e. diisopropylamino-ethyl-O-ethyl methylphosphonothioate Brevundimonas diminuta ?
-
?

Synonyms

Synonyms Comment Organism
phosphotriesterase
-
Brevundimonas diminuta
PTE
-
Brevundimonas diminuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the conjugates display improved thermal stability, with a half-life at 37°C and pH 7.4 increasing 48fold from 0.256 h to 12.3 h for native phosphotriesterase and phosphotriesterase-MPEG 5 kDa, respectively Brevundimonas diminuta
61
-
the melting point of the native enzyme is at 61°C Homo sapiens
73
-
the melting point of the recombinant enzyme is at 73°C Homo sapiens