Application | Comment | Organism |
---|---|---|
medicine | human paraoxonase 1 is a catalytic bioscavenger for pre-treatment and therapy of organophosphorus compounds poisoning | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
General Stability | Organism |
---|---|
human paraoxonase 1 alone is unstable. But it is stabilized by the presence of its most common partner protein, the human phosphate binding protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
paraoxon | native enzyme, in 50 mM borate buffer (pH 8.5, 0.1 mM CoCl2), at 25°C | Brevundimonas diminuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CVX + H2O | i.e. diethylamino-ethyl-O-butyl methylphosphonothioate | Homo sapiens | ? | - |
? | |
CVX + H2O | i.e. diethylamino-ethyl-O-butyl methylphosphonothioate | Brevundimonas diminuta | ? | - |
? | |
paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? | |
paraoxon + H2O | - |
Brevundimonas diminuta | 4-nitrophenol + diethyl phosphate | - |
? | |
VR + H2O | i.e. diethylamino-ethyl-O-isobutyl methylphosphonothioate | Homo sapiens | ? | - |
? | |
VR + H2O | i.e. diethylamino-ethyl-O-isobutyl methylphosphonothioate | Brevundimonas diminuta | ? | - |
? | |
VX + H2O | i.e. diisopropylamino-ethyl-O-ethyl methylphosphonothioate | Homo sapiens | ? | - |
? | |
VX + H2O | i.e. diisopropylamino-ethyl-O-ethyl methylphosphonothioate | Brevundimonas diminuta | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase | - |
Brevundimonas diminuta |
PTE | - |
Brevundimonas diminuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the conjugates display improved thermal stability, with a half-life at 37°C and pH 7.4 increasing 48fold from 0.256 h to 12.3 h for native phosphotriesterase and phosphotriesterase-MPEG 5 kDa, respectively | Brevundimonas diminuta |
61 | - |
the melting point of the native enzyme is at 61°C | Homo sapiens |
73 | - |
the melting point of the recombinant enzyme is at 73°C | Homo sapiens |