Literature summary for 3.1.8.1 extracted from

Tsai, P.C.; Bigley, A.; Li, Y.; Ghanem, E.; Cadieux, C.L.; Kasten, S.A.; Reeves, T.E.; Cerasoli, D.M.; Raushel, F.M.
Stereoselective hydrolysis of organophosphate nerve agents by the bacterial phosphotriesterase (2010), Biochemistry, 49, 7978-7987.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL-21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F132G	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
G60A	the mutant shows increased catalytic efficiency with sarin and soman compared to the wild type enzyme	Homo sapiens
H254Q/H257F	the mutant shows increased catalytic efficiency compared to the wild type enzyme	Homo sapiens
H257Y/L303T	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
I106A/F132A/H257Y	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
I106A/H257Y/S308A	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
I106G	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
I106G/F132G/H257Y	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
I106G/H257Y	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens
S308G	the mutant shows decreased catalytic efficiency compared to the wild type enzyme	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the native enzyme contains two Zn2+ ions, and these metal ions can be substituted with Cd2+, Co2+, Ni2+, or Mn2+ without a loss of catalytic activity	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Ultrogel AcA 54 gel filtration, and DEAE-Sephadex A-25 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	-	?
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	-	?
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	-	?
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	-	?
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + 2-methylpropyl (R)-methylphosphonate	-	?
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + 2-methylpropyl (S)-methylphosphonate	-	?
4-acetylphenyl cyclohexyl (R)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + cyclohexyl (R)-methylphosphonate	-	?
4-acetylphenyl cyclohexyl (S)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + cyclohexyl (S)-methylphosphonate	-	?
4-acetylphenyl ethyl (R)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + ethyl (R)-methylphosphonate	-	?
4-acetylphenyl ethyl (S)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + ethyl (S)-methylphosphonate	-	?
4-acetylphenyl propan-2-yl (R)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + propan-2-yl (R)-methylphosphonate	-	?
4-acetylphenyl propan-2-yl (S)-methylphosphonate + H2O	-	Homo sapiens	4-acetylphenol + propan-2-yl (S)-methylphosphonate	-	?
cyclosarin + H2O	-	Homo sapiens	?	-	?
additional information	the stereochemical preference of the wild-type enzyme with chemical warfare agents is for the RP enantiomers	Homo sapiens	?	-	?
paraoxon + H2O	best substrate	Homo sapiens	4-nitrophenol + diethyl phosphate	-	?
sarin + H2O	-	Homo sapiens	?	-	?
soman + H2O	-	Homo sapiens	?	-	?
tabun + H2O	-	Homo sapiens	?	-	?
VR + H2O	-	Homo sapiens	?	-	?
VX + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
phosphotriesterase	-	Homo sapiens
PTE	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.016	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.12	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.14	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.21	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.41	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.45	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.62	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.81	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.5	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.1	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.2	-	soman	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.2	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.6	-	soman	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.9	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
3.4	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
5.1	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
5.9	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
7.3	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
7.4	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
8.5	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
9.1	-	soman	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
12	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
14	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
15	-	sarin	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
15	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
18	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
19	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
20	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
22	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
25	-	soman	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
37	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
39	-	sarin	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
40	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
50	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
51	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
89	-	soman	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
90	-	sarin	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
92	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
93	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
93	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
100	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
100	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
110	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
110	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
110	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
120	-	sarin	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
120	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
130	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
150	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
150	-	4-acetylphenyl ethyl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
190	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
250	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
290	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
300	-	sarin	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
370	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
410	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
670	-	4-acetylphenyl ethyl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.00094	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.0011	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.003	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.0032	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.0067	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.016	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.021	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.058	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.099	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.1	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.13	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.2	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.22	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.25	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.25	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.49	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
0.59	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.3	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.5	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.5	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.7	-	4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.8	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1.9	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.3	-	4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.4	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
2.5	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
3.1	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
5.8	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
5.9	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
6.2	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
9.5	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
13	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
16	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
21	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
27	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
28	-	4-acetylphenyl cyclohexyl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
28	-	4-acetylphenyl cyclohexyl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
34	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
73	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
75	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
110	-	4-acetylphenyl propan-2-yl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
110	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
130	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
150	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
160	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
180	-	4-acetylphenyl 2-methylpropyl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
180	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
220	-	4-acetylphenyl ethyl (S)-methylphosphonate	mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
490	-	4-acetylphenyl ethyl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
520	-	4-acetylphenyl ethyl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
520	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
580	-	4-acetylphenyl propan-2-yl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
760	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
850	-	4-acetylphenyl 2-methylpropyl (R)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens
1200	-	4-acetylphenyl ethyl (S)-methylphosphonate	wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C	Homo sapiens