Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain HB101 and BL21(DE3) | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inactivation | Saccharolobus solfataricus | |
fensulfothion | - |
Saccharolobus solfataricus | |
o-phenanthroline | inactivation | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | first order kinetics | Saccharolobus solfataricus | |
0.06 | - |
paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus | |
0.205 | - |
methyl paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme depends on divalent metal ions with Co2+, Mg2+, and Ni2+ being the most effective | Saccharolobus solfataricus | |
Mg2+ | the enzyme depends on divalent metal ions with Co2+, Mg2+, and Ni2+ being the most effective | Saccharolobus solfataricus | |
Ni2+ | the enzyme depends on divalent metal ions with Co2+, Mg2+, and Ni2+ being the most effective | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE, x * 35565, sequence calculation, the enzyme is in a monomer-dimer equilibrium | Saccharolobus solfataricus |
35565 | - |
x * 34000, SDS-PAGE, x * 35565, sequence calculation, the enzyme is in a monomer-dimer equilibrium | Saccharolobus solfataricus |
48000 | 50000 | recombinant enzyme, gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ultracentrifugation, anion exchange chromatography, and hydrophobic interaction chromatography | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00042 | - |
purified recombinant enzyme, substrate paraoxon | Saccharolobus solfataricus |
0.0022 | - |
purified recombinant enzyme, substrate methyl paraoxon | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus | 4-nitrophenol + dimethylphosphate | - |
? | |
methyl paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus MT-4 / DSM 5833 | 4-nitrophenol + dimethylphosphate | - |
? | |
additional information | substrate specificity with organophosphorous compounds, overview | Saccharolobus solfataricus | ? | - |
? | |
additional information | substrate specificity with organophosphorous compounds, overview | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? | |
paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus | 4-nitrophenol + diethyl phosphate | - |
? | |
paraoxon + H2O | the enzyme from Sulfolobus solfataricus has a low paraoxonase activity | Saccharolobus solfataricus MT-4 / DSM 5833 | 4-nitrophenol + diethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | x * 34000, SDS-PAGE, x * 35565, sequence calculation, the enzyme is in a monomer-dimer equilibrium | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase | - |
Saccharolobus solfataricus |
PTE | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
and above | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 95 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 85 | purified recombinant enzyme, 4 h, completely stable | Saccharolobus solfataricus |
95 | - |
purified recombinant enzyme, half-life is 4 h | Saccharolobus solfataricus |
100 | - |
purified recombinant enzyme, half-life is 90 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus | |
1.3 | - |
methyl paraoxon | pH 8.0, 70°C, recombinant enzyme | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | broad optimum | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | - |
Saccharolobus solfataricus |