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Literature summary for 3.1.8.1 extracted from
- Mechanism for the hydrolysis of organophosphates by the bacterial phosphotriesterase (2004), Biochemistry, 43, 5707-5715.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Brevundimonas diminuta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D233A | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
| D233N | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
| D301A | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
| D301N | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
| H254A | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
| H254N | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
| Y309F | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of the Zn-enzyme and the Cd-enzyme, wild-type and mutant, kinetic model, overview | Brevundimonas diminuta | |
| 0.3 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta |  |
| 0.34 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 0.5 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 0.6 | - |
parathion | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 0.6 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 0.74 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 1.5 | - |
parathion | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 1.6 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 1.7 | - |
parathion | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 1.9 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 3.3 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 7 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | activates slightly | Brevundimonas diminuta | |
| Zn2+ | the enzyme is a zinc metalloenzyme | Brevundimonas diminuta | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | P0A434 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Brevundimonas diminuta |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol | mechanism of hydrolysis of organophosphates by the bacterial phosphotriesterase, reaction modelling, kinetic model | Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diethyl 4-chlorophenyl phosphate + H2O | - |
Brevundimonas diminuta | 4-chlorophenol + diethyl phosphate | - |
? | |
| diethyl 4-chlorophenyl thiophosphate + H2O | - |
Brevundimonas diminuta | 4-chlorophenol + diethyl thiophosphate | - |
? | |
| diethyl 4-nitrophenyl phosphate + H2O | - |
Brevundimonas diminuta | 4-nitrophenol + diethyl phosphate | - |
? | |
| parathion + H2O | - |
Brevundimonas diminuta | 4-nitrophenol + diethyl thiophosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphotriesterase | - |
Brevundimonas diminuta |
| PTE | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Brevundimonas diminuta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 0.23 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 0.36 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 1.1 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 2.2 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 2.4 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 300 | - |
parathion | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 600 | - |
parathion | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 720 | - |
parathion | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 2060 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 2300 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
| 2500 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Brevundimonas diminuta |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profiles of wild-type and mutant enzymes substituted with different metals, overview | Brevundimonas diminuta |
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