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Literature summary for 3.1.8.1 extracted from

  • Lu, H.; Wheeldon, I.; Banta, S.
    Catalytic biomaterials: Engineering organophosphate hydrolase to form self-assembling enzymatic hydrogels (2010), Protein Eng. Des. Sel., 23, 559-566.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain SG13009 Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Flavobacterium sp. ATCC 27 551
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200pg gel filtration Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
His6-tagged organophosphate hydrolase fused with alpha-helical leucine zipper domains and unstructured soluble linker domains shows specific activities from 1.6-2.2 units for paraoxon, 0.61-1.3 units for parathion and 0.011-0.036 units for ethyl parathion (one unit is defined as mol of substrate per mol enzyme per second), in 50 mM carbonate buffer (pH 10.5), at 25°C Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenolate + H2O
-
Flavobacterium sp. ?
-
?
4-nitrophenolate + H2O
-
Flavobacterium sp. ATCC 27 551 ?
-
?
methyl parathion + H2O
-
Flavobacterium sp. ?
-
?
methyl parathion + H2O
-
Flavobacterium sp. ATCC 27 551 ?
-
?
paraoxon + H2O
-
Flavobacterium sp. 4-nitrophenol + diethyl phosphate
-
?
paraoxon + H2O
-
Flavobacterium sp. ATCC 27 551 4-nitrophenol + diethyl phosphate
-
?
parathion + H2O
-
Flavobacterium sp. ?
-
?
parathion + H2O
-
Flavobacterium sp. ATCC 27 551 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 36000, SDS-PAGE Flavobacterium sp.

Synonyms

Synonyms Comment Organism
OP hydrolase
-
Flavobacterium sp.
OPH
-
Flavobacterium sp.
organophosphate hydrolase
-
Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5 11
-
Flavobacterium sp.