Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain SG13009 | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. ATCC 27 551 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200pg gel filtration | Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
His6-tagged organophosphate hydrolase fused with alpha-helical leucine zipper domains and unstructured soluble linker domains shows specific activities from 1.6-2.2 units for paraoxon, 0.61-1.3 units for parathion and 0.011-0.036 units for ethyl parathion (one unit is defined as mol of substrate per mol enzyme per second), in 50 mM carbonate buffer (pH 10.5), at 25°C | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenolate + H2O | - |
Flavobacterium sp. | ? | - |
? | |
4-nitrophenolate + H2O | - |
Flavobacterium sp. ATCC 27 551 | ? | - |
? | |
methyl parathion + H2O | - |
Flavobacterium sp. | ? | - |
? | |
methyl parathion + H2O | - |
Flavobacterium sp. ATCC 27 551 | ? | - |
? | |
paraoxon + H2O | - |
Flavobacterium sp. | 4-nitrophenol + diethyl phosphate | - |
? | |
paraoxon + H2O | - |
Flavobacterium sp. ATCC 27 551 | 4-nitrophenol + diethyl phosphate | - |
? | |
parathion + H2O | - |
Flavobacterium sp. | ? | - |
? | |
parathion + H2O | - |
Flavobacterium sp. ATCC 27 551 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, SDS-PAGE | Flavobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
OP hydrolase | - |
Flavobacterium sp. |
OPH | - |
Flavobacterium sp. |
organophosphate hydrolase | - |
Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | 11 | - |
Flavobacterium sp. |