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3.1.8.1: aryldialkylphosphatase

This is an abbreviated version!
For detailed information about aryldialkylphosphatase, go to the full flat file.

Word Map on EC 3.1.8.1

Reaction

An aryl dialkyl phosphate
+
H2O
=
dialkyl phosphate
 +
an aryl alcohol

Synonyms

A-esterase, A7Q26_23485, aminopeptidase P, AMPP, aryldialkylphosphatase, arylesterase, aryltriphosphatase, bacterial phosphotriesterase, DFPase, diisopropyl fluorophosphatase, esterase B1, esterase E4, esterase, organophosphate, esterase, paraoxon, esterase, pirimiphos-methyloxon, G3C9 rePON1, h-PON1, HAD, haloalkylphosphorus hydrolase, HDL-associated esterase/lactonase paraoxonase 1, HDL-PON1, high activity paraoxonase, high-density lipoprotein-associated esterase/lactonase, human paraoxonase 1, HuPON1, inner membrane protein YiaH, lactonase SsoPox, lactonase/phosphotriesterase, low activity paraoxonase, methyl parathion hydrolase, More, Mph, mPHP, OP hydrolase, OP-hydrolase, OP-hydrolyzing enzyme, OPA anhydrase, opd, OpdA, OpdD, OPH, OPHC2, organophosphate hydrolase, organophosphorous hydrolase, organophosphorus acid anhydrase, organophosphorus hydrolase, organophosphorus pesticide hydrolase, organophosphorus-hydrolyzing enzyme, paraoxon hydrolase, paraoxonase, paraoxonase 1, paraoxonase 1A, paraoxonase 3, paraoxonase-1, paraoxonase-2, paraoxonase1, parathion hydrolase, phosphotriesterase, phosphotriesterase homology protein, phosphotriesterase-like lactonase, PHP, pirimiphos-methyloxon esterase, PLL, PO.ase, PON, PON 1, PON-1, PON-aryl, PON-para, PON1, PON1A, PON2, PON3, POX, PTE, PTE S5, Rv0230c, SACI2140, Saci_2140, SacPox, Sb-PTE, serum paraoxonase, serum paraoxonase 1, SisLac, SisPox, Sso, SSO2522, SsoPox, type A paraoxonase, type B paraoxonase, VmoLac, Vmut2255, VmutPLL

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.1 aryldialkylphosphatase

Purification

Purification on EC 3.1.8.1 - aryldialkylphosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Sepharose-4B-L-tyrosine-9-aminophenanthrene column chromatography
-
ammonium sulfate precipitation and Sepharose-4Bl-tyrosine-1-naphthylamine column chromatography
-
ammonium sulfate precipitation, DEAE-Sephadex gel filtration and Sephadex G-200 gel filtration
ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration
-
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200pg gel filtration
-
ammonium sulfate precipitation, Ultrogel AcA 54 gel filtration, and DEAE-Sephadex A-25 gel filtration
-
anion-exchange chromatography and concanavalin-A chromatography
blue agarose affinity chromatography and HiTrap DEAE column chromatography
by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, 314.9fold with a yield of 25.3%
-
by ammonium sulfate precipitation and hydrophobic interaction chromatography specifically designed for PON1 enzyme, 901fold with 14.5% yield for R192 isoenzyme and 453fold with 6.9% yield for Q192 isoenzyme
-
by different types of chromatographies
-
by immobilized metal-ion affinity chromatography
-
by ion exchange chromatography, affinity chromatography and ultrafiltration
by pseudo-affinity chromatography and gel filtration
-
by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield
-
ceramic HA column chromatography, DEAE-Sepharose column chromatography, Sephadex G-25 gel filtration, and tert-butyl Sepharose column chromatography
Cibacron Blue 3GA agarose gel chromatography and Q-Sepharose resin chromatography
Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography
co-purification of CAW-tagged OPH and N-terminally His6-tagged TonB
co-purification of PON1 and phosphate binding protein from high-density-lipoprotein-particles using hydroxyapatite chromatography, method development
DEAE-Sepharose column chromatography, Concanavalin A-Sepharose column chromatography, and Sepharose CL-6B gel filtration
HisTrap affinity column chromatography
in the presence of Co2+, by centrifugation and on Co2+ affinity resin, to over 95%
-
membrane preparation from wild-type and mutant cells, solubilization of OPH from the inner membrane using a buffer that contains Triton X-100, Triton X-114, n-dodecyl beta-D-maltoside, and digitonin, followed by dialysis, and immunoaffinity chromatography
native enzyme 227fold by ammonium sulfate fractionation and L-tyrosine-1-naphthylamine hydrophobic interaction chromatography to homogeneity
-
native extracellular serum enzyme by ammonium sulfate fractionation and hydrophobic interaction chromatography on a L-tyrosine and 1-naphthylamine containing resin to homogeneity
-
native PON1 from serum, overview, recombinant mutant enzymes from Escherichia coli, transient expression of mutant enzymes in HEK293 cells
native soluble enzyme 27.3fold by ultracentrifugation, ammonium sulfate precipitation, gel filtration, and ion exchange chromatography to homogeneity
-
nickel agarose affinity column chromatography
-
partial
PON1-HDL complex, overview
-
purification of serum PON1 from different bovine breeds namely Swiss Black, Holstein, and Montofon
-
Q-Sepharose column chromatography
recombinant enzyme
-
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli by ultracentrifugation, anion exchange chromatography, and hydrophobic interaction chromatography
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant enzyme from Escherichia coli strain BL21(DE3) 7.63fold by heat treatment at 66-72°C, by response surface methodology, and ultrafiltration using an automatic tangential flow filtration system, followed by anion exchange chromatography. The ultrafiltration step is preferred compared to a gel filtration step
-
recombinant enzyme is purified by DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography
recombinant enzyme mutant SsoPox 3 M from Escherichia coli strain BL21(DE3) 13.10fold by heat treatment at 66-72°C, by response surface methodology, and ultrafiltration using an automatic tangential flow filtration system, followed by anion exchange chromatography. The ultrafiltration step is preferred compared to a gel filtration step
recombinant His-tagged enzyme 23.4fold from Escherichia coli by nickel affinity chromatography under non-dneturating and under denaturating conditions, overview
recombinant His-tagged enzymes from Sphingobium fuliginis strain KGU0379 and Sphingomonas paucimobilis strain KGU0001 by nickel affinity chromatography
recombinant His-tagged mutant G137D from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography
-
recombinant His6-tagged catalytically active paraoxwild-type and mutant enzymes refolded from inclusion bodies in Escherichia coli strain BL21(DE3) by anion exchange chromatography
recombinant His6-tagged from Escherichia coli strain SG13009 OPH 29-43fold by agarose-based nickel affinity chromatography, and 60fold by affinity chromatography on divalent metal ion-iminodiacetic acid–polyacrylamide cryogel, usage of Ni2+, Co2+ or Cu2+ as methyl ions, method development and evaluation, effect of sample pretreatment on His6-OPH purification, overview
-
recombinant His6-tagged OPH from Escherichia coli by nickel affinity chromatography
-
recombinant human paraoxonase 1 fused to human immunoglobulin Fc domain from Drosophila melanogaster S2 cells by protein A affinity chromatography and dialysis. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant mutant enzyme
recombinant N-terminally His6-tagged enzyme
recombinant N-terminally His6-tagged OPH from Escherichia coli strain B-2935 by Co2+-iminodiacetic acid-polyacrylamide cryogel affinity chromatography
-
recombinant OpdA from Escherichia coli strain DH5alpha by anion exchange chromatography, dialysis, sulphopropyl affinity chromatography, and again dialysis
recombinant OPHC2 from Pichia pastoris strain GS115 culture supernatant by gel filtration and ultrafiltration
recombinant thioredoxin-fusion PON1 genetic variant G2E6 from Escherichia coli, the tag is spontaneously cleaved
recombinant wild-type and genetic variants
recombinant wild-type and mutant enzymes from cell-free enzyme extract by precipitation of nucleic acids by protamine sulfate, ammonium sulfate fractionation, and gel filtration, followed by anion exchange chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by isolating inclusion bodies, refolding of the proteins, and anion exchange chromatography
recombinant wild-type and mutant enzymes refolded from Escherichia coli strain BL21(DE3) inclusion bodies by ion exchange chromatography
the presence of 2.5 mM Ca2+ and 0.1% (w/v) Triton X-100 (as detergent) in the buffers throughout the purification procedure is essential for maintaining the activity of the enzyme. In the absence of calcium and Triton X-100, the enzyme activity is quickly lost
two enzymes, difficult to separate
-
two forms, plasma and liver form
-