Literature summary for 3.1.8.1 extracted from

Samra, Z.Q.; Shabir, S.; Rehmat, Z.; Zaman, M.; Nazir, A.; Dar, N.; Athar, M.A.
Synthesis of cholesterol-conjugated magnetic nanoparticles for purification of human paraoxonase 1 (2010), Appl. Biochem. Biotechnol., 162, 671-686.

Inhibitors

Inhibitors	Comment	Organism
acetaminophen	at 37°C, 5% slightly reduces activity by 18% concentration	Homo sapiens
acetone	at 37°C, 1 mM reduces activity by 82%	Homo sapiens
acetyl salicylic acid	at 37°C, 5% slightly reduces activity by 10% concentration	Homo sapiens
beta-mercaptoethanol	at 37°C, 1 mM reduces activity by 52%	Homo sapiens
Cd2+	at 37°C, 1 mM inhibits activity	Homo sapiens
Co2+	at 37°C, 1 mM reduces activity by 30%	Homo sapiens
Cu2+	at 37°C, 1 mM inhibits activity	Homo sapiens
dimethyl sulfoxide	at 37°C, 5% completely inhibits activity	Homo sapiens
dimethylformamide	at 37°C, 5% completely inhibits activity	Homo sapiens
ethanol	at 37°C, 1 mM reduces activity by 43%	Homo sapiens
gentamicin sulfate	at 37°C, 0.6 mg/ml reduces activity by 50%	Homo sapiens
iodoacetic acid	at 37°C, 5% completely inhibits activity	Homo sapiens
kanamycin sulfate	at 37°C, 0.6 mg/ml reduces activity by 50%	Homo sapiens
additional information	at 37°C, 1 mM Mg2+, 1 mM Mn2+, 5% glycerol, 5% PEG 6000, 5% ammonium sulfate, and 5% phenylmethylsulfonyl fluoride do not inhibit the enzyme activity	Homo sapiens
Ni2+	at 37°C, 1 mM inhibits activity	Homo sapiens
Pb2+	at 37°C, 1 mM reduces activity by 35%	Homo sapiens
sodium ampicillin	at 37°C, 30 mg/ml reduces activity by 50%	Homo sapiens
Sodium dodecyl sulfate	at 37°C, 5% completely inhibits activity	Homo sapiens
streptomycin sulfate	at 37°C, 0.6 mg/ml reduces activity by 50%	Homo sapiens
Zn2+	at 37°C, 1 mM reduces activity by 70%	Homo sapiens

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.3	-	paraoxon	2 mM substrate, at pH 8.0 and 37°C	Homo sapiens

Metals/Ions	Comment	Organism	Structure
additional information	at 37°C, 1 mM Mg2+ and Mn2+ have no stimulatory effect on activity	Homo sapiens

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, SDS-PAGE	Homo sapiens

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Comment)	Organism
by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield	Homo sapiens

Source Tissue	Comment	Organism	Textmining
plasma	-	Homo sapiens	-

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.85	-	plasma, at 37°C	Homo sapiens
438.2	-	515fold purified enzyme, at 37°C	Homo sapiens

Storage Stability	Organism
4°C, 20% glycerol	Homo sapiens

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
paraoxon + H2O	-	Homo sapiens	4-nitrophenol + diethyl phosphate	-	?

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE	Homo sapiens

Synonyms	Comment	Organism
paraoxonase 1	-	Homo sapiens
PON1	-	Homo sapiens

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Homo sapiens

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	is stable for 8 to 10 h at 37°C. Completely loses activity at 50°C	Homo sapiens

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Homo sapiens

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	activity is reduced below pH 7.0 and at alkaline pH	Homo sapiens

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	purified PON1, isoelectric focusing	-	5.3