Cloned (Comment) | Organism |
---|---|
gene mpd, subcloning in Escherichia coli strain DH5alpha, expression of functional His-tagged enzyme in Escherichia coli strain BL21(DE3) improved by elimination of the signal peptide | Plesiomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33700 | - |
recombinant His-tagged mature enzyme, gel filtration | Plesiomonas sp. |
35000 | - |
1 * 35000, recombinant His-tagged mature enzyme, SDS-PAGE | Plesiomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plesiomonas sp. | Q9ALW1 | gene mpd | - |
Plesiomonas sp. M6 | Q9ALW1 | gene mpd | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme precursor contains a 36 amino acid N-terminal signal peptide | Plesiomonas sp. |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 23.4fold from Escherichia coli by nickel affinity chromatography under non-dneturating and under denaturating conditions, overview | Plesiomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53.8 | - |
purified recombinant His-tagged mature enzyme | Plesiomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl parathion + H2O | - |
Plesiomonas sp. | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
methyl parathion + H2O | - |
Plesiomonas sp. M6 | 4-nitrophenol + dimethyl thiophosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, recombinant His-tagged mature enzyme, SDS-PAGE | Plesiomonas sp. |
More | the enzyme precursor contains a 36 amino acid N-terminal signal peptide which is cleaved off to give the mature, active enzyme | Plesiomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
methyl parathion hydrolase | - |
Plesiomonas sp. |
Mph | - |
Plesiomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Plesiomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Plesiomonas sp. |